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ALX-630-027 Revised 09-Oct-08
α-Latrotoxin
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Neurotoxins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-630-027-C040   40 µg 450.00 USD Add To Cart
Product Specification
MW: ~130kDa.
CAS NUMBER: 65988-34-3
RTECS: OE9020000
SOURCE/HOST: Isolated from Latrodectus tredecimguttatus.
QUANTITY: Determined by Lowry and Pierce method.
PURITY: ≥97%
APPEARANCE: Lyophilized.
PURITY DETAIL: Migrates as one distinct band when run on a 5-25% gradient polyacrylamide gel according to the method of Laemmli (Nature 227, 680 (1970)).
RECONSTITUTION: Reconstitute in 0.5ml of distilled cold water. Stir very gently. Do not shake or vortex. Add 0.5 ml glycerol. Stock solution received contains 50% glycerol and 300nM α-latrotoxin.
QUALITY CONTROL: Bioassay: Test of the ability to stimulate neurotransmitter release in both Ca2+-free and Ca2+-containing media according to Valtorta. Effective concentration: 100pM-1nM.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stock solution is stable for 1 week when stored at +4°C or for at least one year at -20°C.
HAZARD: TOXIC.
Product Description
Causes massive neurotransmitter release from a wide variety of central and peripheral synaptic junctions of vertebrates using Ca2+-dependent and Ca2+-independent pathways. A useful pharmacological tool in the studies of synaptic vesicles exocytosis of different neutrotransmitters.
Product Specific Literature References
Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions: N. Frontali, et al.; J. Cell. Biol. 68, 462 (1976) Abstract
Preparation and properties of a neurotoxin purified from the venom of black widow spider (Latrodectus mactans tredecimguttatus): A. Grasso; Biochim. Biophys. Acta 439, 406 (1976) Abstract
Synaptophysin (p38) at the frog neuromuscular junction: its incorporation into the axolemma and recycling after intense quantal secretion: F. Valtorta, et al.; J. Cell. Biol. 107, 2717 (1988) Abstract
The effect of alpha-latrotoxin on a synaptic connection between identified neurons in the brain of the mollusc Helix pomatia L: O.N. Osipenko, et al.; Toxicon 31, 1123 (1993) Abstract
GTP cleavage by the small GTP-binding protein Rab3A is associated with exocytosis of synaptic vesicles induced by alpha-latrotoxin: B. Stahl, et al.; J. Biol. Chem. 269, 24770 (1994) Abstract; Full Text
Alpha-latrotoxin stimulates glutamate release from cortical astrocytes in cell culture: V. Parpura, et al.; FEBS Lett. 360, 266 (1995) Abstract
Mechanisms of alpha-latrotoxin action: A. W. Henkel & S. Sankaranarayanan; Cell Tissue Res. 296, 229 (1999), (Review) Abstract
alpha-Latrotoxin and its receptors: neurexins and CIRL/latrophilins: T.C. Sudhof; Annu. Rev. Neurosci. 24, 933 (2001), (Review) Abstract
Alpha-latrotoxin induces exocytosis by inhibition of voltage-dependent K+ channels and by stimulation of L-type Ca2+ channels via latrophilin in beta-cells: S. Lajus, et al.; J. Biol. Chem. 281, 5522 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Attention: For best results, only proceed according to the given instructions. Dissolution and/or storage of this toxin contrary to these instructions may affect the stability and biological activity of this compound.
Further Categories Containing This Product:
Ca2+ Modulators • Exocytosis
 
 
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