ALEXIS Biochemicals: APO-DIRECT™ Kit

ALX-850-041

Revised 29-Jan-05

APO-DIRECT™ Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	DNA-based Apoptosis Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-041-KI01		1 Kit	540.00 USD

Product Specification

QUANTITY:	50 assays.
KIT/SET CONTAINS:	Contains all the reagents necessary, including positive and negative control cells.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	+4°C
POSITIVE CONTROL INCLUDED:	yes

Product Description

One-step flow cytometry kit for the qualitative and quantitative measurement of apoptosis in mammalian cells. One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini of DNA ends which can be identified with fluorescent tagged deoxyuridine triphosphate nucleotides (F-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates (e.g. F-dUTP) to the 3'h-hydroxyl ends of double- or single-stranded DNA. A substantial number of these sites are available in apoptotic cells providing the basis for the single step fluorescent labelling and flow cytometric method utilized in the Apo-DIRECT™ Kit.

Product Specific Literature References

Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry 13, 795 (1992) Abstract
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry 27, 1 (1997), (Review) Abstract
Single-step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides: detection of apoptosis and bromodeoxyuridine incorporation: X. Li, et al.; Cytometry 20, 172 (1995) Abstract

General Information

MANUFACTURER

Manufactured by Phoenix Flow Systems.

Further Categories Containing This Product:

Detection Kits

header picture © micronaut.ch, another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.