ALEXIS Biochemicals: Ac-LEVD-AFC

ALX-260-084

Revised 24-Feb-05

Ac-LEVD-AFC
SYNONYMS	Caspase-4 Substrate (Fluorogenic)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Substrates

Ordering Information

Product Specification

SEQUENCE:	Ac-Leu-Glu-Val-Asp-AFC (AFC=7-Amido-4-trifluoromethylcoumarin)
FORMULA:	C₃₂H₄₀F₃N₅O₁₁
MW:	727.7
PEPTIDE CONTENT:	75-95%.
PURITY:	≥99% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO. Dilute with distilled water or buffer. Also soluble in phosphate buffer, pH 7.5-8.0.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Dissolve only as much as needed at one time because of the possibility of decomposition in solution. Store solution at -20°C. Protect from condensation: when taking the sample out of the container, wait until it warms up to room temperature, then open it.
HANDLING:	Keep cool and dry.

Product Description

Fluorogenic substrate for caspase-4. Similar to the fluorogenic substrate Ac-LEVD-AMC (Prod. No. ALX-260-083); the AFC fluorophore is more sensitive.

General Information

BACKGROUND/TECHNICAL INFORMATION

AFC has an excitation maximum of 400nm and an emission maximum of 505nm.

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