ALEXIS Biochemicals: Adenylate Cyclase Toxin

ALX-630-088

Revised 03-Apr-08

Adenylate Cyclase Toxin
SYNONYMS	AC Toxin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Toxins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-630-088-C050		50 µg	290.00 USD

Product Specification

MW:	~177kDa.
SOURCE/HOST:	Recombinant Bordetella pertussis adenylate cyclase toxin produced in E. coli.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥75% (SDS-PAGE)
APPEARANCE:	Lyophilized.
SOLUBILITY:	Soluble in water.
RECONSTITUTION:	Reconstitute with 50µl sterile distilled water to get a solution in 20mM HEPES, pH 7.5, containing 8M urea. The resulting suspension should be made uniform by gentle mixing prior to use.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	After reconstitution, store at concentrations higher than 100µg/ml to reduce adherence to the container surface.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C.
HAZARD:	TOXIC.

Product Description

Important virulence factor of Bordetella pertussis and a research tool for manipulation of cAMP levels in mammalian cells. Has the ability to interact with target cells, insert into the cytoplasmic membrane and deliver its adenylate cyclase enzymatic domain to the cell interior.

Product Specific Literature References

[1] Calmodulin activates prokaryotic adenylate cyclase: J. Wolff, et al.; PNAS 77, 3841 (1980) Abstract
[2] Phagocyte impotence caused by an invasive bacterial adenylate cyclase: D.L. Confer & J.W. Eaton; Science 217, 948 (1982) Abstract
[3] Virulence factors of Bordetella pertussis: A. Weiss & E.L. Hewlett; Ann. Rev. Microbiol. 40, 661 (1986) Abstract
[4] The calmodulin-sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia coli: P. Glaser, et al.; Mol. Microbiol. 2, 19 (1988) Abstract
[5] Adenylate cyclase toxin from Bordetella pertussis. Identification and purification of the holotoxin molecule: E.L. Hewlett, et al.; J. Biol. Chem. 264, 19379 (1989) Abstract
[6] Bordetella pertussis adenylate cyclase toxin. Structural and functional independence of the catalytic and hemolytic activities: H. Sakamoto, et al.; J. Biol. Chem. 267, 13598 (1992) Abstract; Full Text
[7] Characterization of adenylate cyclase toxin from a mutant of Bordetella pertussis defective in the activator gene, cyaC: E.L. Hewlett, et al.; J. Biol. Chem. 268, 7842 (1993) Abstract; Full Text
[8] Calmodulin-activated bacterial adenylate cyclases as virulence factors: M. Mock & A. Ullmann; Trends in Microbiol. 1, 187 (1993) Abstract
[9] Adenylate cyclase toxin (CyaA) of Bordetella pertussis. Evidence for the formation of small ion-permeable channels and comparison with HlyA of Escherichia coli: R. Benz, et al.; J. Biol. Chem. 269, 27231 (1994) Abstract; Full Text
[10] Internal lysine palmitoylation in adenylate cyclase toxin from Bordetella pertussis: M. Hackett, et al.; Science 266, 433 (1994) Abstract
[11] Adenylate cyclase toxin from Bordetella pertussis produces ion conductance across artificial lipid bilayers in a calcium- and polarity- dependent manner: G. Szabo, et al.; J. Biol. Chem. 269, 22496 (1994) Abstract; Full Text
[12] Hemolytic, but not cell-invasive activity, of adenylate cyclase toxin is selectively affected by differential fatty-acylation in escherichia coli: M. Hackett, et al.; J. Biol. Chem. 270, 20250 (1995) Abstract; Full Text
[13] Bordetella pertussis adenylate cyclase toxin: a versatile screening tool: N. Dautin, et al.; Toxicon 40, 1383 (2002), (Review) Abstract
[14] The adenylate cyclase toxin from Bordetella pertussis--a novel promising vehicle for antigen delivery to dendritic cells: M. Simsova, et al.; Int. J. Med. Microbiol. 239, 571 (2004), (Review) Abstract
[15] Bordetella adenylate cyclase toxin: a swift saboteur of host defense: J. Vojtova, et al.; Curr. Opin. Microbiol. 9, 69 (2006) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Enzymatic activity: The enzymatic activity of this preparation is >238µmol cAMP/min/mg of protein as determined by a modification of the method of Hewlett et al. [5].
Toxicity: The toxicity of this preparation is >63µmol cAMP/mg of J774A.1 cell protein as determined by a modification of the method of Hewlett et al. [7].

Further Categories Containing This Product:

Enzymes • Adenylyl Cyclase Activators

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