ALEXIS Biochemicals: Annexin V (human) (recombinant) (ATTO 647N)

ALX-209-259

Revised 11-Oct-06

Annexin V (human) (recombinant) (ATTO 647N)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Annexin V/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-209-259-T100		100 tests	260.00 USD

Product Specification

MW:	~52kDa (SDS-PAGE).
QUANTITY:	100 tests/0.5ml
PURITY:	≥90%
PURITY DETAIL:	Affinity purified.
FORMULATION:	Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
SPECIFICITY:	Binds to phosphatidylserine (PS).
APPLICATION:	Detection of apoptotic cells by flow cytometry or immunofluorescence.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least one year after receipt when stored at +4°C.
HANDLING:	Protect from light. Do not freeze.

Product Description

New ATTO-fluorescent dyes show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 647N shows red fluorescence (λabs (max): 645nm, λem (max): 669nm, εmax: 120’000).

General Information

BACKGROUND/TECHNICAL INFORMATION

Flow Cytometry Protocol:

- Wash cells in PBS, and resuspend in binding buffer (10mM HEPES/NaOH, pH 7.4, 140mM NaCl, 2.5mM CaCl₂; filtered through 0.2µm filter).
- Adjust cells to a density between 200’000 and 500’000 cells/ml.
- Take 195µl cell suspension and add 5µl Annexin-ATTO 647N. Mix and incubate 10 min. in the dark.
- Wash cells 1x and resuspend in 190µl binding buffer.
- Add 10µl of 20µg/ml propidium iodide (PI) stock solution to stain for necrotic cells.
- Analyze by FACS.
Note: These are general recommendations. Optimal conditions must be determined individually for each application:

Swiss-Prot link P08758: Annexin V (human)
AfCS Signalling Gateway link A000281: Annexin V (mouse)

MANUFACTURER

Manufactured by Apotech Corporation.

ALX-209-252	Annexin V (human) (recombinant) (APC)
ALX-209-256	Annexin V (human) (recombinant) (FITC)
ALX-209-251	Annexin V (human) (recombinant) (R-PE)
ALX-209-250	Annexin V (human) (recombinant) (FITC)