ALEXIS Biochemicals: ApoSENSOR™ ADP/ATP Ratio Assay Kit

ALX-850-248

Revised 22-Apr-08

ApoSENSOR™ ADP/ATP Ratio Assay Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Apoptosis & Necrosis Detection Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-248-KI01		200 tests	455.00 USD

Product Specification

QUANTITY:	200 assays.
KIT/SET CONTAINS:	50ml Nucleotide Releasing Buffer, 1 vial ATP Monitoring Enzyme, 1 vial ADP Converting Enzyme, 500µl Enzyme Reconstitution Buffer.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Protect from light.

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Product Description

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
The assay utilizes bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light fom ATP and luciferin. The light can be measured using a luminometer or beta counter. ADP levels are measured by its conversion to ATP that is subsequently detected using the same reaction.
This highly sensitive assay (detects 100 mammalian cells/well) can be fully automated for high throughput measurements. The ADP/ATP ratio assay offers highly consistent results, with excellent correlation to other apoptosis markers (e. g. TUNEL-based techniques and caspase assays).

General Information

MANUFACTURER

Manufactured by BioVision.

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