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ALX-300-141 Revised 07-Jul-08
Arachidonoyl Thio-PC
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SYNONYMS 2-Deoxy-2-thioarachidonoylphosphatidylcholine
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Phospholipids/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-300-141-M005   5 mg 189.00 USD Add To Cart
Product Specification
FORMULA: C44H82NO6PS
MW: 784.2
CAS NUMBER: 146797-82-2
PURITY: ≥98%
FORMULATION: Liquid. Solution in ethanol containing 0.1% BHT.
SOLUBILITY: Soluble in methanol, DMSO or dimethyl formamide; slightly soluble in water (0.5 mg/ml).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at –20°C.

Product Description
Substrate for all forms of phospholipase A2 (PLA2) including the cytosolic, Ca2+-dependent form (cPLA2). Upon hydrolysis of the thioester bond, free thiols are detected using DTNB (Ellmans reagent) (Prod. No. ALX-400-034). For an assay kit for cPLA2 based on this product see Prod. No. ALX-850-009.
Product Specific Literature References
1-Hexadecyl-2-arachidonoylthio-2-deoxy-sn-glycero-3-phosphorylcholine as a substrate for the microtiterplate assay of human cytosolic phospholipase A2: L.J. Reynolds, et al.; Anal. Biochem. 217, 25 (1994) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice. Addition of Triton X-100 will enhance the solubility significantly. The critical micellar concentration of Arachidonoyl Thio-PC is 2mM in 8mM Hepes (pH 7.4), 150mM NaCl, 10mM CaCl2, 4mM Triton X-100, 30% glycerol and 1mg/ml BSA.
Further Categories Containing This Product:
PLA2 Substrates
 
 

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