ALEXIS Biochemicals: Bisbenzimide H33258 . trihydrochloride

ALX-620-051

Revised 30-Jun-08

Bisbenzimide H33258 . trihydrochloride
SYNONYMS	HOE 33258 2'-4-(Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bis-1H-benzimidazole . 3HCl
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Dyes / Stains / Fluorescent Probes

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-051-M010		10 mg	22.00 USD
ALX-620-051-M050		50 mg	35.00 USD

Product Specification

FORMULA:	C₂₅H₂₄N₆O . 3HCl
MW:	424.4 . 109.4
CAS NUMBER:	23491-45-4
RTECS:	SM1140500
PURITY:	≥98% (TLC)
APPEARANCE:	Yellow to greenish powder.
SOLUBILITY:	Soluble in water (10mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
HAZARD:	MAY BE MUTAGENIC. HARMFUL.
IDENTITY:	Identity determined by IR and ¹H-NMR.

Product Description

Cell permeable fluorescent dye with low cytotoxicity. Useful for staining DNA, chromosomes and nuclei [1,2]. Can be used with chromomycin A₃ (Prod. No. ALX-380-055) to distinguish chromosomes by their total DNA concentration and their DNA base composition [3,4]. Binds to A:T pairs in DNA. Also useful for controlling gene expression through binding to RNA aptamers inserted into 5' untranslated region of a messenger RNA [5]. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles [6]. Ex(max): 346nm; Em(max): 460nm.

Product Specific Literature References

[1] Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis: S.A. Latt & G. Stetten; J. Histochem. Cytochem. 24, 24 (1976) Abstract
[2] Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations: K.H. Elstein & R.M. Zucker; Exp. Cell. Res. 211, 322 (1994) Abstract
[3] Bacterial characterization by flow cytometry: M.A. Van Dilla, et al.; Science 220, 620 (1983) Abstract
[4] Determination of guanine-plus-cytosine content of bacterial DNA by dual-laser flow cytometry: C.A. Sanders, et al.; J. Gen. Microbiol. 136, 359 (1990) Abstract
[5] Controlling gene expression in living cells through small molecule-RNA interactions: G. Werstuck & M.R. Green; Science 282, 296 (1998) Abstract
[6] Activation and inhibition of the sarcoplasmic reticulum Ca2+ channel by the polycationic dyes Hoechst 33342 and Hoechst 33258: T.J. Beeler & K. Gable; J. Membr. Biol. 135, 109 (1993) Abstract