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Bone Metabolism
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ALX-804-851 Revised 03-May-07
Monoclonal Antibody to RGMC (human) (Fery-1)
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SYNONYMS anti-Repulsive Guidance Molecule C (human) MAb (Fery-1)
anti-Hemojuvelin (human) MAb (Fery-1)
anti-Hemochromatosis Type 2 Protein (human) MAb (Fery-1)
anti-HFE2 (human) MAb (Fery-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Dependence Receptors Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-851-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Fery-1
ISOTYPE: Mouse IgG2b
SOURCE/HOST: Purified from concentrated hybridoma tissue cell culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RGMC (aa 36-398) (Prod. No. ALX-522-104).
SPECIFICITY: Recognizes human RGMC.
APPLICATION: Flow Cytometry
Immunoprecipitation
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Bone Morphogenetic Proteins [BMPs] & Receptors/Related Products • Polyclonal Antibodies • Growth, Survival and Development of Neurons Other Products
 
 
ALX-804-533 Revised 20-Feb-07
Monoclonal Antibody to TRAP5 (human) (TRAP-01)
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SYNONYMS anti-Tartrate-resistant Acid Phosphatase 5 (human) MAb (TRAP-01)
anti-TRACP (human) MAb (TRAP-01)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY TRAP5 [TRACP]
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ALX-804-533-C100   100 µg 508.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: TRAP-01
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified. Sterile filtered.
FORMULATION: Lyophilized from PBS, pH 7.4.
RECONSTITUTION: Reconstitute with 100µl of distilled water. Activity is not affected by slight turbidity. Clarify by centrifugation.
IMMUNOGEN: Recombinant human TRAP5 (tartrate-resistant acid phosphatase 5).
SPECIFICITY: Recognizes human TRAP5.
APPLICATION: ELISA
ImmunoCapture Enzyme-Activity Assay
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
[1] Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen: J.J. Stepan, et al.; BBRC 165, 1027 (1989) Abstract
[2] Tartrate-resistant acid phosphatase 5b: a novel serum marker of bone resorption: J.M. Halleen, et al.; J. Bone Miner. Res 15, 1337 (2000) Abstract
[3] Tartrate-resistant acid phosphatase isoform 5b as serum marker for osteoclastic activity: A.J. Janckila, et al.; Clin. Chem. 47, 74 (2001) Abstract
[4] Clinical performance of immunoreactive tartrate-resistant acid phosphatase isoform 5b as a marker of bone resorption: R.A. Hannon, et al.; Bone 34, 187 (2004) Abstract
[5] TRACP as an osteopontin phosphatase: G. Andersson, et al.; J. Bone Miner. Res 18, 1912 (2003) Abstract
General Information
TRAP5b is claimed to be a specific marker of bone resorption [2-4]. Both osteopontin and bone sialoprotein are colocalized with TRAP5 in the resorption lacuna of osteoclasts and dephosphorylation of osteopontin impair its ability to promote adhesion as well as migration of osteoclasts in vitro [5].
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-021 Revised 03-Mar-05
Monoclonal Antibody to Vitamin D3 Receptor (9A7)
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SYNONYMS anti-VDR MAb (9A7)
PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Vitamin D3 & Vitamin D3 Receptor/Related Products
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ALX-804-021-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: 9A7
ISOTYPE: Rat IgG2b
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Purified antibody containing 0.05% sodium azide.
IMMUNOGEN: Partially purified chicken intestinal cytoplasmic VDR (vitamin D3 receptor).
SPECIFICITY: Recognizes the occupied and unoccupied forms of VDR in human, mouse, rat, hamster, chicken, amphibian and fish. Recognizes an epitope between aa 89 and 105 of human VDR. In avians detects bands of ~58kDa and ~60kDa by Western blot, while in mammalian species the VDRs appear as a single band within the ~52-55kDa range.
Does not cross-react with ER (estrogen receptor) or GR (glucocorticoid receptor).
APPLICATION: Gel Supershift Assays
Immunocytochemistry
Immunohistochemistry (paraffin sections (5µg/ml))
Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1 alpha,25-dihydroxyvitamin D3 receptor: J.W. Pike, et al.; PNAS 79, 7719 (1982) Abstract
Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. Generation by a preparation enriched in a 64,000-dalton protein: J.W. Pike, et al.; J. Biol. Chem. 258, 1289 (1983) Abstract; Full Text
Immunocytochemical localization of the 1,25-dihydroxyvitamin D3 receptor in target cells: T.L. Clemens, et al.; Endocrinology 122, 1224 (1988) Abstract
Molecular biology of the vitamin D hormone: M.R. Haussler, et al.; Recent Progr. Horm. Res. 44, 263 (1988) Abstract
Immunohistochemical detection of 1,25-dihydroxyvitamin D3 receptors and estrogen receptors by monoclonal antibodies: comparison of four immunoperoxidase methods: P. Milde, et al.; J. Histochem. Cytochem. 37, 1609 (1989) Abstract
Baculovirus-mediated expression of the human vitamin D receptor. Functional characterization, vitamin D response element interactions, and evidence for a receptor auxiliary factor: P.N. MacDonald, et al.; J. Biol. Chem. 266, 18808 (1991) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-201-174 Revised 18-May-06
Osteoblast Specific Factor 1 (human) (recombinant)
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SYNONYMS OSF-1 (human) (recombinant)
Pleiotrophin (human) (recombinant)
HB-GAM (human) (recombinant)
HARP (human) (recombinant)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Heparin Binding Factors/Related Products
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ALX-201-174-C100   100 µg 532.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in E. coli. Human OSF-1 (osteoblast specific factor 1) is fused at the N-terminus to a thrombin cleveage site and a His-tag.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Purified by two-step procedure using Ni-NTA and size exclusion chromatography before and after refolding.
FORMULATION: Lyophilized from sterile filtered solution in 0.01M phosphate buffer, pH 7.2, containing 0.1M sodium chloride.
RECONSTITUTION: Reconstitute with 200µl PBS, pH 7.2.
APPLICATION: ELISA
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted protein is stable for 2 weeks when stored at +4°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
OSF-1 is an extracellular matrix-associated protein, present in matrices, which act as targets for the deposition of new bone. OSF-1 has the ability to promote adhesion, migration, expansion and differentation of human osteoprogenitor cells.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-201-175 Revised 29-Jan-08
Osteoblast Specific Factor 2 (human) (recombinant)
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SYNONYMS OSF-2 (human) (recombinant)
Periostin (human) (recombinant)
Fasciclin-I-like (human) (recombinant)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Heparin Binding Factors/Related Products
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ALX-201-175-C100   100 µg 532.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in E. coli. Human OSF-2 (osteoblast specific factor 2) is fused at the N-terminus to a Xa cleavage site and a His-tag.
CONCENTRATION: 0.5mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
PURITY DETAIL: Three-step procedure using affinity Ni-NTA chromotography and size exclusion chromatography before and after refolding.
FORMULATION: Lyophilized from sterile filtered solution in 0.05M acetate buffer, pH 4.0.
RECONSTITUTION: Reconstitute with 200µl of 0.1M acetate buffer, pH 4.0. If a higher pH is required, dilute to a concentration of ~10µg/ml. Solubility is limited in higher concentrations.
APPLICATION: ELISA, Western Blot.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
OSF-2 functions as a cell adhesion molecule for pro-osteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Patients with bone metastases from breast (but not lung) cancer show elevated levels of OSF-2. OSF-2 expression is significantly increased by bone morphogenetic protein 2 (BMP-2).
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-522-007 Revised 30-Jul-07
Osteoprotegerin (human):Fc (human) (recombinant)
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SYNONYMS OPG (human):Fc (human) (recombinant)
TNFRSF 11B (human):Fc (human) (recombinant)
Osteoclastogenesis Inhibitory Factor (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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ALX-522-007-C050   50 µg 340.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region of human OPG (osteoprotegerin) (aa 22-202) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human and mouse RANKL and TRAIL.
BIOLOGICAL ACTIVITY: Inhibits rhsTRAIL-mediated apoptosis at a concentration range of 5-20µg/ml, as well as rhsRANKL-induced osteoclastogenesis and stimulation of dendritic cells. Concentrations of rhOPG:Fc required to inhibit may vary depending on the cell type studied and on the concentration of rhsTRAIL used to kill the cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
A CD40-CD95L fusion protein interferes with CD40L-induced prosurvival signaling and allows membrane CD40L-restricted activation of CD95: C. Assohou-Luty, et al.; J. Mol. Med. 84, 785 (2006) Abstract
General Information
Osteoprotegerin (OPG) is a secreted tumor necrosis factor receptor homologue that inhibits osteoclastogenesis and increases bone density in vivo. OPG contains 4 cysteine-rich repeats and 2 ‘death domains’ (DD). OPG binds to RANKL (TRANCE) and with a similar affinity to TRAIL.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O00300: Osteoprotegrin (human) (precursor)
AfCS Signalling Gateway link A002819: Osteoprotegrin (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-850-280 Revised 20-Jun-08
Osteoprotegerin (human) ELISA Kit
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SYNONYMS OPG (human) ELISA Kit
TNFRSF 11B (human) ELISA Kit
Osteoclastogenesis Inhibitory Factor (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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ALX-850-280-KI01   1 Kit 1'550.00 USD Add To Cart
Product Specification
SENSITIVITY: 2.8pg/ml
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human OPG (osteoprotegerin) in serum, plasma and urine.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
An increased osteoprotegerin serum release characterizes the early onset of diabetes mellitus and may contribute to endothelial cell dysfunction: P. Secchiero, et al.; Am. J. Pathol. 169, 2236 (2006) Abstract; Full Text
Osteoprotegerin increases leukocyte adhesion to endothelial cells both in vitro and in vivo: G. Zauli, et al.; Blood 110, 536 (2007) Abstract
Activation of the p53 pathway down-regulates the osteoprotegerin (OPG) expression and release by vascular endothelial cells: P. Secchiero, et al.; Blood 111, 1287 (2008) Abstract
General Information
MANUFACTURER Manufactured by Immundiagnostik AG.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-210-312 Revised 26-Mar-08
Polyclonal Antibody to Bone Sialoprotein
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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ALX-210-312-R020   20 µl 135.00 USD Add To Cart
ALX-210-312-R100   100 µl 660.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 20μl or 100μl distilled water.
IMMUNOGEN: Purified human bone sialoprotein (bone extract).
SPECIFICITY: Recognizes human and rat bone sialoprotein. Does not cross-react with human osteopontin or osteonectin.
APPLICATION: ELISA
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Western Blot
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-311 Revised 30-May-07
Polyclonal Antibody to Bone Sialoprotein (human)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-311-C010   10 µg 130.00 USD Add To Cart
ALX-210-311-C100   100 µg 577.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From chicken.
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized. Contains PBS, pH 7.4.
RECONSTITUTION: Reconstitute with distilled water.
IMMUNOGEN: Purified human bone sialoprotein (bone extract).
SPECIFICITY: Recognizes human bone sialoprotein. Does not cross-react with human osteopontin, osteonectin or bone alkaline phosphatase.
APPLICATION: ELISA (1µg/ml)
Flow Cytometry
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (2ng/ml))
Western Blot (2ng/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay: M. Karmatschek, et al.; Clin. Chem. 43, 2076 (1997) Abstract; Full Text
Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients: A. Bellahcene, et al.; Br. J. Haematol. 111, 1118 (2000) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-313 Revised 14-Nov-06
Polyclonal Antibody to Bone Sialoprotein (human)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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ALX-210-313-R020   20 µl 135.00 USD Add To Cart
ALX-210-313-R100   100 µl 660.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 20µl/100µl distilled water.
IMMUNOGEN: Synthetic peptide corresponding to aa 262-279 (Y262ESENGEPRGDNYRAYED279) of human bone sialoprotein.
SPECIFICITY: Recognizes human bone sialoprotein. Does not cross-react with human osteonectin or osteopontin.
APPLICATION: ELISA (1:1'000)
Immunocytochemistry (1:100)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.