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ALX-805-058
Revised 11-Apr-05
Monoclonal Antibody to CD2 (human) (MEM-65)
SYNONYMS
anti-LFA-2 (human) MAb (MEM-65)
anti-T11 (human) MAb (MEM-65)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD2 [LFA-2] & CD58 [LFA-3] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-058-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-65
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS, pH 7.4, containing 0.1% sodium azide.
IMMUNOGEN:
Human peripheral T cells.
SPECIFICITY:
Recognizes a unique epitope of human CD2 (LFA-2) implicated in T cell activation.
APPLICATION:
Flow Cytometry
Immunoprecipitation
Western Blot
Functional Application (studying CD2 ligand-binding mechanisms involved with T cell development and activation)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
New monoclonal antibodies to human leucocyte surface molecule CD2:
K. Drbal, et al.; Folia Biol.
43
, 245 (1997)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-057
Revised 05-Sep-06
Monoclonal Antibody to CD3 (human) (MEM-57)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD3 - Marker for T Cells
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-057-C100
100 µg
195.00 USD
ALX-805-057F-T100
FITC
100 tests
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-57
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
MEM-57
: 1mg/ml.
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
MEM-57
: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
MEM-57-FITC
: Liquid. In PBS, pH 7.4, containing 15mM sodium azide and 0.2% BSA.
IMMUNOGEN:
Human thymocytes and T lymphocytes.
SPECIFICITY:
Recognizes the ε and δ chain of human CD3.
APPLICATION:
Flow Cytometry (20µl per 100µ of whole blood or 10
6
cells in suspension)
Immunoprecipitation
Functional Application (activates T cells
in vitro
; highly effective immunosuppressive agent)
SHIPPING:
SHIPPED ON BLUE ICE
USE/STABILITY:
MEM-57
: Short Term Storage: +4°C. Stable for 6 months.
Long Term Storage: -20°C. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
MEM-57-FITC
: Store at +4°C. Do not freeze! Protect from light!
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74):
V. Horejsi, et al.; Folia Biol. (Praha)
34
, 23 (1988)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-804-822
Revised 18-Aug-08
Monoclonal Antibody to CD3 (human) (TR66)
SYNONYMS
anti-CD3 (human) MAb (TR66)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD3 - Marker for T Cells
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-822-C100
100 µg
190.00 USD
ALX-804-822TD-T100
ATTO 488
100 tests
250.00 USD
ALX-804-822TS-T100
ATTO 647N
100 tests
250.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
TR66
ISOTYPE:
Mouse IgG1
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
>95% (SDS-PAGE).
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Cloned human helper T cell line.
SPECIFICITY:
Recognizes the ε-chain of human CD3. Detects endogenous protein by FC, ICC and IP.
APPLICATION:
Flow Cytometry
Immunocytochemistry
Immunoprecipitation
Functional Application: T cell stimulation
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
The use of hybrid hybridomas to target human cytotoxic T lymphocytes
:
A. Lanzavecchia & D. Scheidegger; Eur. J. Immunol.
17
, 105 (1987)
Abstract
FLIP prevents apoptosis induced by death receptors but not by perforin/granzyme B, chemotherapeutic drugs, and gamma irradiation
:
T. Kataoka, et al.; J. Immunol.
161
, 3936 (1998)
Abstract
;
Full Text
T lymphocyte costimulation mediated by reorganization of membrane microdomains
:
A. Viola, et al.; Science
283
, 680 (1999)
Abstract
CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation
:
O. Gaide, et al.; Nat. Immunol.
3
, 836 (2002)
Abstract
T cell fitness determined by signal strength
:
A.V. Gett, et al.; Nat. Immunol.
4
, 355 (2003)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
1 test means: 1µl of MAb is used to stain 500’000 cells in a sample volume of 50µl.
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-053
Revised 25-Nov-08
Monoclonal Antibody to CD4 (human) (MEM-115)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD4 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-053-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-115
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified from ascites.
FORMULATION:
Liquid. In TBS, pH 8.0, containing 15mM sodium azide.
IMMUNOGEN:
Human thymocytes and T lymphocytes.
SPECIFICITY:
Recognizes an epitope in the D1 domain of human CD4.
APPLICATION:
Flow Cytometry
Immunoprecipitation
Functional Application (blocks binding of HIV-1 gp120 protein to CD4 and inhibits HLA class II interactions)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-054
Revised 19-Apr-06
Monoclonal Antibody to CD4 (human) (MEM-241)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD4 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-054-C100
100 µg
195.00 USD
ALX-805-054F-T100
FITC
100 tests
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-241
ISOTYPE:
Mouse IgG1
CONCENTRATION:
MEM-241
: 1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
MEM-241
: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
MEM-241-FITC
: Liquid. In PBS, pH 7.4, containing 15mM sodium azide and 0.2% BSA.
IMMUNOGEN:
CD4-fusion protein.
SPECIFICITY:
Recognizes human CD4.
APPLICATION:
MEM-241
:
Flow Cytometry (excellent, 1µg/ml; non-reducing conditions)
Western Blot (non-reducing conditions)
MEM-241-FITC
:
Flow Cytometry (excellent) (20µl/10
6
cells or 100µl of whole blood)
SHIPPING:
SHIPPED ON BLUE ICE
USE/STABILITY:
MEM-241
: Short Term Storage: +4°C. Long Term Storage: -20°C. Avoid freeze/thaw cycles. After opening, aliquot and store at -20°C.
MEM-241-FITC
: Long Term Storage: +4°C. Do not freeze! Protect from light!
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-048
Revised 20-Nov-07
Monoclonal Antibody to CD5 (human) (MEM-32)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-048-C100
100 µg
195.00 USD
ALX-805-048F-T100
FITC
100 tests
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-32
ISOTYPE:
Mouse IgG1
CONCENTRATION:
MEM-32
: 1mg/ml
PURITY:
MEM-32
: Liquid. In PBS, pH 7.4, containing 15mM sodium chloride.
MEM-32-FITC
: Liquid. In PBS, pH 7.4, containing 15mM sodium chloride and 0.2% BSA.
IMMUNOGEN:
Crude human thymus membrane fraction.
SPECIFICITY:
Recognizes human CD5.
APPLICATION:
Flow Cytometry (20µl per 100µl whole blood or 10
6
cells in suspension)
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
USE/STABILITY:
Purified antibody:
Short term storage at +4°C. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.
FITC-conjugate:
Store at +4°C. Do not freeze! Protect from light!
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen
:
V. Horejsi, et al.; Folia Biol. (Praha)
32
, 12 (1986)
Abstract
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74):
V. Horejsi, et al.; Folia Biol (Praha)
34
, 23 (1988)
Abstract
Further Categories Containing This Product:
B Cell Receptor [BCR] & Co-Receptors / Related Products
•
Autoimmunity & Self Tolerance Other Products
•
Monoclonal Antibodies
ALX-805-056
Revised 18-Jan-06
Monoclonal Antibody to CD8 (human) (MEM-87)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD8 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-056-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-87
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN:
Leukocytes of a patient suffering from LGL type leukemia.
SPECIFICITY:
Recognizes human CD8.
APPLICATION:
Flow Cytometry
Immunoprecipitation (excellent for immunoisolation of CD8
+
T cells)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-055
Revised 28-Feb-06
Monoclonal Antibody to CD8 (human) (MEM-31)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD8 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-055-C100
100 µg
195.00 USD
ALX-805-055F-T100
FITC
100 tests
270.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-31
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
MEM-31
: 1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
MEM-31
: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
MEM-31-FITC
: Liquid. In PBS, pH 7.4, containing 15mM sodium
azide and 0.2% BSA.
IMMUNOGEN:
Crude human thymus membrane fraction.
SPECIFICITY:
Recognizes human CD8.
APPLICATION:
MEM-31
:
Flow Cytometry
Immunoprecipitation
MEM-31-FITC
:
Flow Cytometry (20µl/10
6
cells or 100µl of whole blood)
SHIPPING:
SHIPPED ON BLUE ICE
USE/STABILITY:
MEM-31
: Short Term Storage: +4°C. Long Term Storage: -20°C. Avoid freeze/thaw cycles. After opening, aliquot and store at -20°C.
MEM-31-FITC
: Long Term Storage: +4°C. Do not freeze! Protect from light!
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen
:
V. Horejsi, et al.; Folia Biol. (Praha)
32
, 12 (1986)
Abstract
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74):
V. Horejsi, et al.; Folia Biol (Praha)
34
, 23 (1988)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-805-027
Revised 20-Jan-06
Monoclonal Antibody to CD9 (human) (Gi15)
SYNONYMS
anti-p24 (human) MAb (Gi15)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-027-C100
100 µg
230.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
Gi15
ISOTYPE:
Mouse IgG2b
PURITY:
>95%.
FORMULATION:
Liquid. 100µg of purified antibody in 200µl PBS containing 0.05% sodium azide.
IMMUNOGEN:
Human platelets.
SPECIFICITY:
Recognizes human CD9 (p29).
APPLICATION:
Immunoprecipitation
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-805-061
Revised 15-Apr-08
Monoclonal Antibody to CD11a (human) (MEM-25)
SYNONYMS
anti-αL Integrin (human) MAb (MEM-25)
anti-LFA-1 (human) MAb (MEM-25)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
CD11a, CD18 [LFA-1] & CD54 [ICAM-1], CD102 [ICAM-2], CD50 [ICAM-3] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-805-061-C100
100 µg
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MEM-25
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 15mM (~0.1%) sodium azide.
IMMUNOGEN:
Leukocytes from a patient suffering from a LGL-type leukaemia.
SPECIFICITY:
Recognizes the α subunit (CD11a; αL integrin) of human LFA-1.
APPLICATION:
Flow Cytometry
Immunoprecipitation
Functional Application (blocks binding of CD54 (ICAM-1); suitable for affinity purification of LFA-1 from leukocytes)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Specific Literature References
Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein
:
V. Bazil, et al.; Folia Biol. (Praha)
36
, 41 (1990)
Abstract
Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins:
L. Ottonello, et al.; Blood
93
, 3505 (1999)
Abstract
;
Full Text
Further Categories Containing This Product:
Integrins / Related Products
•
Monoclonal Antibodies
•
CD Antibodies
ALX-804-552
Revised 29-Jan-08
Monoclonal Antibody to CD14 (mouse) (biG 57)
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
CD14 / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-552-C100
100 µg
400.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
biG 57
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml after reconstitution.
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Lyophilized. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 100µl distilled water.
IMMUNOGEN:
Recombinant mouse CD14.
SPECIFICITY:
Recognizes mouse CD14.
APPLICATION:
ELISA
Flow Cytometry
Note:
Does not inhibit binding of LPS to CD14.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C