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RANK & RANKL/OPG/Related Products
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Items 19 of 19
ALX-550-516 Revised 21-May-08
Compound A
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SYNONYMS CpdA
2-((4-Acetophenyl)-2-chloro-N-methyl)ethylammonium chloride
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-550-516-M005   5 mg 40.00 USD Add To Cart
ALX-550-516-M025   25 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C11H14ClNO2 . HCl
MW: 227.7 . 36.5
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or water.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stock solutions in ethanol are stable for up to 1 month when stored at -80°C.
HANDLING: Protect from moisture. After reconstitution, prepare aliquots and store at -80°C.

Product Description
Cell permeable, nonsteroidal glucocorticoid receptor modulator with high binding affinity. Exerts anti-inflammatory potential by down-modulating TNF-induced pro-inflammatory gene expression, such as IL-6 and E-selectin. Unlike dexamethasone (Prod. No. ALX-370-002) it does not enhance glucocorticoid response element (GRE)-driven gene expression or induce glucocorticoid receptor (GR) binding to GRE-dependent genes in vivo.
Product Specific Literature References
A fully dissociated compound of plant origin for inflammatory gene repression: K. De Bosscher, et al.; PNAS 102, 15827 (2005) Abstract
A plant-derived ligand favoring monomeric glucocorticoid receptor conformation with impaired transactivation potential attenuates collagen-induced arthritis: P. Dewint, et al.; J. Immunol. 180, 2608 (2008) Abstract
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Further Categories Containing This Product:
GITR & GITRL Other Products • Glucocorticoids & Glucocorticoid Receptors/Related Products • RANK & RANKL/OPG Other Products • NOS Inhibitors (NOS Induction & Enzyme Activity) • DNA & RNA Methyl & Alkyl Transferases/Related Products • Antitumor Agents (Hormone-related) • Natural Products - Nitric Oxide Pathway Modulators • Natural Products - Chemopreventive Agents • Natural Products for Cell Cycle Research • Other Natural Products - DNA Regulation/Transcription
 
 
ALX-370-002 Revised 19-Sep-06
Dexamethasone
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SYNONYMS 9α-Fluoro-16α-methylprednisolone
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors/Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-370-002-G001   1 g 45.00 USD Add To Cart
ALX-370-002-M250   250 mg 22.00 USD Add To Cart
Product Specification
FORMULA: C22H29FO5
MW: 392.5
CAS NUMBER: 50-02-2
MERCK INDEX: 14: 2943
RTECS: TU3980000
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or methanol. Slightly soluble in acetone or chloroform. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.
HAZARD: HARMFUL. MAY BE TERATOGENIC.

Product Description
Inhibits constitutive OPG mRNA steady-state levels in a dose- and time-dependent fashion, promoting osteoclast formation. Glucocorticoid with anti-inflammatory activity. Inhibits the induction of nitric oxide synthases (NOS).
Product Specific Literature References
Inhibition of the induction of nitric oxide synthase by glucocorticoids: yet another explanation for their anti-inflammatory effects?: S. Moncada & R.M.J. Palmer; TIPS 12, 130 (1991), (Review) Abstract
Glucocorticoids inhibit the induction of nitric oxide synthase and the related cell damage in adenocarcinoma cells: K.J. O'Connor & S. Moncada; Biochim. Biophys. Acta 1097, 227 (1991) Abstract
Nitric oxide mediates tumor necrosis factor-alpha cytotoxicity in endothelial cells: C. Estrada, et al.; BBRC 186, 475 (1992) Abstract
Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium: R.R. Dudek, et al.; BBRC 202, 1120 (1994) Abstract
Induction of the alkyltransferase (MGMT) gene by DNA damaging agents and the glucocorticoid dexamethasone and comparison with the response of base excision repair genes: T. Grombacher, et al.; Carcinogenesis 17, 2329 (1996) Abstract
Activation of human O6-methylguanine-DNA methyltransferase gene by glucocorticoid hormone: T. Biswas, et al.; Oncogene 18, 525 (1999) Abstract
Dexamethasone-induced thymocyte apoptosis: apoptotic signal involves the sequential activation of phosphoinositide-specific phospholipase C, acidic sphingomyelinase, and caspases: M.G. Cifone, et al.; Blood 93, 2282 (1999) Abstract; Full Text
Dexamethasone induces apoptosis in human T cell clones expressing low levels of Bcl-2: M.S. Montani, et al.; Cell Death Differ 6, 79 (1999) Abstract
Dexamethasone inhibits the phosphorylation of retinoblastoma protein in the suppression of human vascular smooth muscle cell proliferation: T.D. Reil, et al.; J. Surg. Res. 92, 108 (2000) Abstract
Dexamethasone suppresses iNOS gene expression by inhibiting NF-kappaB in vascular smooth muscle cells: M. Matsumura, et al.; Life Sci. 69, 1067 (2001) Abstract
Dexamethasone inhibits inducible nitric-oxide synthase expression and nitric oxide production by destabilizing mRNA in lipopolysaccharide-treated macrophages: R. Korhonen, et al.; Mol. Pharmacol. 62, 698 (2002) Abstract; Full Text
Molecular mechanisms underlying dexamethasone inhibition of iNOS expression and activity in C6 glioma cells: J. Shinoda, et al.; Glia 42, 68 (2003) Abstract
Dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-kappaB activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line: A.L. Vital, et al.; Mediators Inflamm. 12, 71 (2003) Abstract
Dexamethasone suppresses eNOS and CAT-1 and induces oxidative stress in mouse resistance arterioles: S.C. Schafer, et al.; Am. J. Physiol. Heart Circ. Physiol. 288, H436 (2005) Abstract; Full Text
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Further Categories Containing This Product:
NOS Inhibitors (NOS Induction & Enzyme Activity) • GITR & GITRL Other Products • RANK & RANKL/OPG Other Products • Antitumor Agents (Hormone-related) • Chemopreventive Agents Other Products • DNA & RNA Methyl & Alkyl Transferases/Related Products • Cell Cycle Other Products
 
 
ALX-522-131 Revised 27-Mar-08 New product
Fc (human):RANKL, Soluble (mouse) (rec.)
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SYNONYMS Fc (human):ODF, Soluble (mouse) (recombinant)
Fc (human):OPGL, Soluble (mouse) (recombinant)
Fc (human):TRANCE, Soluble (mouse) (recombinant)
Fc (human):TNFSF 11, Soluble (mouse) (recombinant)
Fc (human):CD254, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-522-131-C010   10 µg 390.00 USD Add To Cart
Product Specification
MW: ~55kDa
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse RANKL (aa 157-316) is fused to the Fc portion of human IgG1.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to mouse RANK.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O35235: TNF11 (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
APO-54N-028 Revised 02-Apr-08
Free Osteoprotegerin (human) Detection Set [For ELISA Application]
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SYNONYMS Free OPG (human) Detection Set [For ELISA Application]
Free TNFRSF 11B (human) Detection Set [For ELISA Application]
Free Osteoclastogenesis Inhibitory Factor (human) Detection Set [For ELISA Application]
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
APO-54N-028-KI01   1 Set 540.00 USD Add To Cart
Product Specification
SENSITIVITY: 2pg/ml (range 0 to 250 pg/ml).
QUANTITY: 5x96 wells.
KIT/SET CONTAINS: 1 vial Standard (lyophilized) (STD).
1 vial Coating Antibody (COAT).
1 vial Detection Antibody (DET).
APPLICATION: For the quantitative determination of human free osteoprotegerin (not bound to RANKL or TRAIL) in cell culture supernatant, plasma and serum.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
OPG (Osteoprotegerin; also called Osteoclastogenesis Inhibitory Factor or TNFRSF11B) is a secreted member of the TNF (Tumor necrosis factor) receptor superfamily of proteins [1]. OPG, composed of 380 amino acids, is expressed as monomer and secreted as disulphide-homodimeric protein [2]. Produced by numerous cell types and tissues, its expression pattern targets the skeletal, immune, and vascular systems. Osteoblasts secrete OPG that protects the skeleton from excessive bone resorption by acting as a decoy receptor for the ligand nuclear factor-κB ligand (RANKL), preventing it from interacting with its receptor RANK [3]. OPG/RANK/RANKL molecular triumvirat plays a pivotal function in the regulation of the immune system through dendritic cells [4]. OPG-deficient mice exhibit severe osteoporosis and vascular calcification of the aorta and renal arteries [5]. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD), a bone disease [6]. OPG also functions as a soluble receptor for the TNF ligand TRAIL (TNF-related apoptosis-inducing ligand) preventing it to activate apoptotic signaling cascase and therefore providing cancer cells with a survival advantage [7].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O00300: Osteoprotegerin (human)
General Literature References
[1] Biology of RANK, RANKL, and osteoprotegerin: B.F. Boyce & L. Xing; Arthritis Res. Ther. 9 Suppl 1, S1 (2007) Abstract
[2] Osteoprotegerin: a novel secreted protein involved in the regulation of bone density: W.S. Simonet, et al.; Cell 89, 309 (1997) Abstract
[3] Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation: D.L. Lacey, et al.; Cell 93, 165 (1998) Abstract
[4] RANKL, RANK, osteoprotegerin: key partners of osteoimmunology and vascular diseases: M. Baud huin, et al.; Cell Mol. Life Sci. 64, 2334 (2007) Abstract
[5] Osteoprotegerin reverses osteoporosis by inhibiting endosteal osteoclasts and prevents vascular calcification by blocking a process resembling osteoclastogenesis: H. Min, et al.; J. Exp. Med. 192, 463 (2000) Abstract
[6] Paget’s disease of bone and genetic disorders of RANKL/OPG/RANK/NF-kappaB signaling: M.P. Whyte; Ann. N. Y. Acad. Sci. 1068, 143 (2006) Abstract
[7] Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL: J.G. Emery, et al.; J. Biol. Chem. 273, 14363 (1998) Abstract; Full Text
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-804-813 Revised 26-Oct-07
Monoclonal Antibody to Osteoprotegerin (human) (Bony-1)
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SYNONYMS anti-OPG (human) MAb (Bony-1)
anti-TNFRSF 11B (human) MAb (Bony-1)
anti-Osteoclastogenesis Inhibitory Factor (human) MAb (Bony-1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-813-C100   100 µg 320.00 USD Add To Cart
ALX-804-813B-C100 Biotin 100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Bony-1
ISOTYPE: Rat IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human OPG (osteoprotegerin) cysteine-rich region (aa 22-202) (Prod. No. ALX-522-007).
SPECIFICITY: Recognizes human OPG.
APPLICATION: ELISA
Flow Cytometry (1:1’000)
Immunoprecipitation (1:200)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Osteoprotegerin: U. Feige; Ann. Rheum. Dis. 60, Suppl 3, iii81 (2001) Abstract; Full Text
Osteoprotegerin is a soluble decoy receptor for tumor necrosis factor-related apoptosis-inducing ligand/Apo2 ligand and can function as a paracrine survival factor for human myeloma cells: C.M. Shipman and P.I. Croucher; Cancer Res. 63, 912 (2003) Abstract; Full Text
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-207 Revised 13-Dec-04
Monoclonal Antibody to Osteoprotegerin (human) (NT) (OPG-13)
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SYNONYMS anti-OPG (human) MAb (NT) (OPG-13)
anti-TNFRSF 11B (human) MAb (OPG-13)
anti-Osteoclastogenesis Inhibitory Factor (human) MAb (NT) (OPG-13)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-207-C100   100 µg 508.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: OPG-13
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized from sterile filtered solution of protein G affinity purified antibody in 0.05M phosphate buffer, pH 7.2, containing 0.1M sodium chloride.
RECONSTITUTION: Reconstitute with 100µl of distilled water. Activity is not affected by slight turbidity. Clarify by centrifugation.
IMMUNOGEN: Synthetic peptide corresponding to N-terminal aa 21-39 of human OPG (osteoprotegerin).
SPECIFICITY: Recognizes human OPG.
APPLICATION: ELISA
Western Blot (1:10'000).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 aa with molecular weight of 55kDa as a monomer and 110kDa as a disulphide-linked dimer. It acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-532 Revised 15-Apr-08
Monoclonal Antibody to Osteoprotegerin (human) (OPG-01)
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SYNONYMS anti-OPG (human) MAb (OPG-01)
anti-TNFRSF 11B (human) MAb (OPG-01)
anti-Osteoclastogenesis Inhibitory Factor (human) MAb (OPG-01)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-532-C100   100 µg 508.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: OPG-01
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized from sterile filtered solution in 0.05M phosphate buffer, pH 7.2, containing 0.1M sodium chloride.
RECONSTITUTION: Reconstitute with 100µl distilled water. Activity is not affected by slight turbidity. Clarify by centrifugation.
IMMUNOGEN: Recombinant human OPG (osteoprotegerin).
SPECIFICITY: Recognizes human OPG.
APPLICATION: ELISA
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 aa with molecular weight of 55kDa as a monomer and 110kDa as a disulfide-linked dimer. It acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-212 Revised 17-Sep-07
Monoclonal Antibody to RANK (human) (9A725)
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SYNONYMS anti-TRANCE-R (human) MAb (9A725)
anti-ODFR (human) MAb (9A725)
anti-TNFRSF 11A (human) MAb (9A725)
anti-CD265 (human) MAb (9A725)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-212-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 9A725
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human RANK (aa 326-616).
SPECIFICITY: Recognizes an epitope between aa 330-427 of human RANK. Detects a band of ~97kDa by Western blot.
APPLICATION: Flow Cytometry (intracellular) (see [1])
Immunohistochemistry (paraffin sections)
Western Blot  (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
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Product Specific Literature References
[1] Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
[2] A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004) Abstract
[3] Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism: A.C. Bharti, et al.; J. Biol. Chem. 279, 6065 (2004) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-244 Revised 20-Aug-07
Monoclonal Antibody to RANKL (12A380)
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SYNONYMS anti-ODF MAb (12A380)
anti-OPGL MAb (12A380)
anti-TRANCE MAb (12A380)
anti-TNFSF 11 MAb (12A380)
anti-CD254 MAb (12A380)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-244-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 12A380
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse RANKL (aa 1-317).
SPECIFICITY: Recognizes human and mouse RANKL. Detects a band of ~40kDa by Western blot.
APPLICATION: Flow Cytometry (see [2])
Immunohistochemistry (frozen sections (see [2]))
Western Blot (4-6µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Product Specific Literature References
[1] Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
[2] Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis: T. Yoneda, et al.; Endocrinology 145, 2384 (2004)