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Monoclonal Antibodies
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ALX-804-720 Revised 29-May-07
Monoclonal Antibody to Adiponectin (rat) (RADI 264)
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SYNONYMS anti-ACRP30 (rat) MAb (RADI 264)
anti-Adipocyte Complement Related Protein of 30kDa (rat) MAb (RADI 264)
anti-AdipoQ (rat) MAb (RADI 264)
anti-apM1 (rat) MAb (RADI 264)
anti-GBP28 (rat) MAb (RADI 264)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Adiponectin [ACRP30] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-720-C050   50 µg 195.00 USD Add To Cart
ALX-804-720-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
CLONE: RADI 264
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant rat adiponectin.
SPECIFICITY: Recognizes rat adiponectin. Does not cross-react with human or mouse adiponectin.
APPLICATION: ELISA (direct and indirect: 1:5’000-1:10’000)
Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-028 Revised 12-Jun-08
Monoclonal Antibody to ADP-ribosylation Factor (1D9)
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SYNONYMS anti-ARF MAb (1D9)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY ADP Ribosylation Factor [ARF] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-028-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
CLONE: 1D9
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human ARF1 (ADP-ribosylation factor-1).
SPECIFICITY: Recognizes human, mouse, rat, dog and hamster ARF1, ARF3, ARF5 and ARF6. Detects a band of ~21kDa by Western blot.
APPLICATION: Immunocytochemistry
Immunohistochemistry (1:500)
Immunoprecipitation
Western Blot (1:500)
Note: Not suitable for neutralizing ARF in solution.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane: M.M. Cavenagh, et al.; J. Biol. Chem. 271, 21767 (1996) Abstract; Full Text
Further Categories Containing This Product:
Vesicles & Membrane Trafficking Other Products • Monoclonal Antibodies
 
 
ALX-803-048 Revised 07-Mar-06
Monoclonal Antibody to Proadrenomedullin (human) (140/2A7)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Hormones & Hormone Receptors Other Products
Ordering Information
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ALX-803-048-C100   100 µg 300.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 140/2A7
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Ammonium sulphate precipitation.
FORMULATION: Liquid. In PBS, pH 7.4, containing 0.9mg/ml sodium azide.
IMMUNOGEN: Recombinant human pre-proadrenomedullin (CR31KKWNKWALSRGKNELRMS49).
SPECIFICITY: Recognizes an epitope (aa 31-52) of human proadrenomedullin.
APPLICATION: ELISA
Radioimmunoassay
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-801-090 Revised 28-Oct-08
Monoclonal Antibody to α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) (M86)
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PRODUCT LINE Immunology
PRODUCT CATEGORY Xenotransplantation
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ALX-801-090-L002   3 ml 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Pig
Others
CLONE: M86
ISOTYPE: Mouse IgM
FORMULATION: Liquid. Supernatant antibody.
IMMUNOGEN: Rabbit red blood cell membrane.
SPECIFICITY: Recognizes synthetic and naturally produced mouse, rat and pig Galα1-3Gal epitopes on glycoproteins and glycolipids. Does not cross-react with β-Gal glycoproteins or BSA.
APPLICATION: ELISA
Flow Cytometry
Immunohistochemistry (paraffin sections)
Western Blot
Functional Application (can be used for cytotoxicity assays for α-Gal specific pathways)
Note: Do not use non-fat milk for blocking! Many blocking agents from non-human mammal sources will cross-react with the antibody.
An antibody dilution of 1:5  is recommended. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Stable for at least 3 years when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Description
Widely described antibody against α-galactose residues with broad cross-reactivity among different species. Useful for measuring the α-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimicks in vivo reactions and can be used for cytotoxicity assays for α-Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity.
Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-α-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to α-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR. The monoclonal antibody M86 to Galα1-3Gal epitopes developed by U. Galili, et al. is an essential tool in the study of human xenotransplantation and related research.
Product Specific Literature References
A sensitive assay for measuring alpha-Gal epitope expression on cells by a monoclonal anti-Gal antibody: U. Galili, et al.; Transplantation 65, 1129 (1998) Abstract
Regulation of natural killer cell-mediated swine endothelial cell lysis through genetic remodeling of a glycoantigen: S. Miyagawa, et al.; J. Biochem. 126, 1067 (1999) Abstract
alpha-Gal Oligosaccharides: Chemistry and Potential Biomedical Application: A. Janczuk, et al.; Current Med. Chem. 6, 155 (1999) Abstract
Mechanism of delayed rejection in transgenic pig-to-primate cardiac xenotransplantation: R.H. Chen, et al.; J. Surg. Res. 90, 119 (2000) Abstract
Xenotransplantation: in vitro analysis of synthetic alpha-galactosyl inhibitors of human anti-Galalpha1-->3Gal IgM and IgG antibodies: R. Rieben, et al.; Glycobiology 10, 141 (2000) Abstract
Differential immune responses to alpha-gal epitopes on xenografts and allografts: implications for accommodation in xenotransplantation: M. Tanemura, et al.; J. Clin. Invest. 105, 301 (2000) Abstract
Differential expression of alpha-Gal epitopes on pig and mouse organs: M. Tanemura & U. Galili; Transplant. Proc. 32, 843 (2000) Abstract
The alpha-gal epitope (Gal alpha 1-3Gal beta 1-4GlcNAc-R) in xenotransplantation: U. Galili; Biochemie 83, 557 (2001) Abstract
Remodeling of the Major Pig Xenoantigen by N-Actetylglucosaminyltransferase III in Transgenic Pig: S. Miyagawa, et al.; J. Biol. Chem. 276, 39310 (2001) Abstract; Full Text
Synthesis of α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) on human tumor cells by recombinant α1,3galactosyltransferase produced in Pichia pastoris: Z.C. Chen, et al.; Glycobiology 11, 577 (2001) Abstract; Full Text
Differential expression of Galalpha1,3Gal epitopes on fetal and adult porcine hematopoietic cells: S. Gojo, et al.; Xenotransplantation 9, 297 (2002) Abstract; Full Text
Expression of alpha-gal epitopes on HeLa cells transduced wtih adenovirus containing alpha-1,3galactosyltransferase cDNA: L. Deriy, et al.; Glycobiology 12, 135 (2002) Abstract
Lack of Galactose-alpha-1,3-Galactose Expression on Porcine Endothelial Cells Prevents Complement-Induced Lysis but Not Direct Xenogeneic NK Cytotoxicity: B.C. Baumann, et al.; J. Immunol. 172, 6460 (2004) Abstract
Further Categories Containing This Product:
CD40 & CD40L [CD154] / Related Products • Monoclonal Antibodies
 
 
ALX-803-311 Revised 21-Oct-08
Monoclonal Antibody to Aggrecan (5C5)
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SYNONYMS anti-Chondroitin Sulfate Proteoglycan MAb (5C5)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Proteoglycans / Related Products
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ALX-803-311-R100   100 µl 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: 5C5
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites.
IMMUNOGEN: Human articular cartilage aggrecan (chondroitinase ABC/keratanase II/endo-β-galactosidase digested).
SPECIFICITY:

Recognizes an epitope within the globular region of human and bovine aggrecan. Does not cross-react with rat, chicken or shark aggrecan.
APPLICATION: ELISA (1:500-1:1’000)
Immunohistochemistry (paraffin sections (1:50-1:300)): Predigestion of tissue sections with 100-150mU/ml testicular hyaluronidase or rather crude collagenases (1-2 hours at 37°C) may improve staining.
Immunoprecipitation (1:50-1:300)
Western Blot (1:100-1:300; reducing and non-reducing conditions)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-313 Revised 14-Feb-08
Monoclonal Antibody to  Aggrecan (5D3)
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SYNONYMS anti-Chondroitin Sulfate Proteoglycan MAb (5D3)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Proteoglycans / Related Products
Ordering Information
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ALX-803-313-R100   100 µl 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: 5D3
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites.
IMMUNOGEN: Human articular cartilage aggrecan (chondroitinase ABC/keratanase II/endo-β-galactosidase digested).
SPECIFICITY: Recognizes human and bovine aggrecan. Does not cross-react with rat, chicken or shark aggrecan.
APPLICATION: ELISA (1:500-1:1'000)
Immunohistochemistry (paraffin sections (1:50-1:300))
Immunoprecipitation (1:50-1:300)
Western Blot (1:100-1:300)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-312 Revised 17-Mar-08
Monoclonal Antibody to Aggrecan (6F4)
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SYNONYMS anti-Chondroitin Sulfate Proteoglycan MAb (6F4)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Proteoglycans / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-803-312-R100   100 µl 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: 6F4
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites.
IMMUNOGEN: Human articular cartilage aggrecan (chondroitinase ABC/keratanase II/endo-β-galactosidase digested).
SPECIFICITY: Recognizes human and bovine aggrecan. Does not cross-react with rat, chicken or shark aggrecan.
APPLICATION: ELISA (1:500-1:'000)
Immunohistochemistry (paraffin sections (1:50-1:300): Predigestion of tissue sections with 100-150mU/ml testicular hyaluronidase or rather crude collagenases (1-2 hours at 37°C) may improve staining.
Immunoprecipitation (1:50-1:300)
Western Blot (1:100-1:300; reducing and non-reducing conditions)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-108 Revised 18-Sep-07
Monoclonal Antibody to Ah Receptor Nuclear Translocator (2B10)
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SYNONYMS anti-ARNT MAb (2B10)
anti-HIF-1β MAb (2B10)
anti-Hypoxia-inducible Factor 1β MAb (2B10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Aryl Hydrocarbon Receptor / Related Products
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ALX-804-108-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 2B10
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 771-789 (N771SYNNEEFPDLTMFPPFSE789) of human ARNT (Ah receptor nuclear translocator).
SPECIFICITY: Recognizes human, mouse and monkey ARNT. Detects a band of ~87kDa by Western blot.
APPLICATION: Immunocytochemistry (1:1'000)
Immunoprecipitation
Western Blot (1:4'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Physiochemical and immunocytochemical analysis of the aryl hydrocarbon receptor translocator: Characterization of two monoclonal antibodies to the ARNT: N.G. Hord & G. Perdew; Mol. Pharmacol. 46, 618 (1994) Abstract
Oxygen tension modulates beta-globin switching in embryoid bodies: S. Bichet, et al.; FASEB J. 13, 285 (1999) Abstract; Full Text
Induction and nuclear translocation of hypoxia-inducible factor-1 (HIF-1): heterodimerization with ARNT is not necessary for nuclear accumulation of HIF-1alpha: D. Chilov, et al.; J. Cell Sci. 112, 1203 (1999) Abstract; Full Text
Protein kinase C modulates aryl hydrocarbon receptor nuclear translocator protein-mediated transactivation potential in a dimer context: W.P. Long, et al.; J. Biol. Chem. 274, 12391 (1999) Abstract; Full Text
DNA methylation represses the expression of the human erythropoietin gene by two different mechanisms: H. Yin and K.L. Blanchard; Blood 95, 111 (2000) Abstract; Full Text
A new HIF-1 alpha variant induced by zinc ion suppresses HIF-1-mediated hypoxic responses: Y.S. Chun, et al.; J. Cell Sci. 114, 4051 (2001) Abstract; Full Text
Synergistic cooperation between hypoxia and transforming growth factor-beta pathways on human vascular endothelial growth factor gene expression: T. Sanchez-Elsner, et al.; J. Biol. Chem. 276, 38527 (2001) Abstract; Full Text
Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia: H. Suzuki, et al.; Oncogene 20, 5779 (2001) Abstract; Full Text
Rescue of hypoxia-inducible factor-1alpha-deficient tumor growth by wild-type cells is independent of vascular endothelial growth factor: G. Höpfl, et al.; Cancer Res. 62, 2962 (2002) Abstract; Full Text
The arylhydrocarbon receptor (AhR), but not the AhR-nuclear translocator (ARNT), is increased in hearts of patients with cardiomyopathy: M.R. Mehrabi, et al.; Virchows Arch. 441, 481 (2002) Abstract; Full Text
Further Categories Containing This Product:
Hypoxia-inducible Factor [HIF] / Related Products • Monoclonal Antibodies
 
 
ALX-804-283 Revised 10-Mar-08
Monoclonal Antibody to AIB1 (human) (AX15.3)
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SYNONYMS anti-Amplified in Breast Cancer 1 (human) MAb (AX15.3)
anti-SRC-3 (human) MAb (AX15.3)
anti-Steroid Receptor Coactivator-3 (human) MAb (AX15.3)
anti-NCOA3 (human) MAb (AX15.3)
anti-Nuclear Receptor Coactivator 3 (human) MAb (AX15.3)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nuclear Receptor Coactivators / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-283-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: AX15.3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Purified antibody in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant human AIB1 (amplified in breast cancer 1) (aa 605-1294).
SPECIFICITY: Recognizes human AIB1. Detects a band of ~155kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (2µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1: D.O. Azorsa, et al.; Hybridoma 18, 28 (1999) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-400 Revised 20-Sep-07
Monoclonal Antibody to Akt (dephosphorylated) (11A11)
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SYNONYMS anti-PKB (dephosphorylated) MAb (11A11)
anti-Protein Kinase B (dephosphorylated) MAb (11A11)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-400-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 11A11
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal (QFSYSA) of human Akt (protein Kinase B; PKB) dephosphorylated at Ser472/473/474.
SPECIFICITY: Recognizes human, mouse, rat and dog Ser472/473/474-dephosphorylated Akt (see technical note). Does not cross-react with Ser472/473/474-phosphorylated Akt.
APPLICATION: Immunohistochemistry
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:

Included (Prod. No. ALX-840-030).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% aa identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. The phosphorylation site slightly differs, thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2:  L461LELDQRTHFPQFS474YSASIRE481        Swiss-Prot-Link: P31751
Akt3:  D461NERRPHFPQFS472YSASGRE479         Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-553 Revised 01-Dec-05
Monoclonal Antibody to Akt (5C10)