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ALX-804-361
Revised 09-May-07
Monoclonal Antibody to NF-κB (p65) (112A1021)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Transcription Factors / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-361-C100
100 µg
293.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
112A1021
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 526-539 of human NF-κB (p65 subunit) .
SPECIFICITY:
Recognizes human and mouse NF-κB (p65 subunit ). Detects a band of ~65kDa by Western blot.
APPLICATION:
Flow Cyctometry (2.5µg/ml) (see [1])
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Specific Literature References
[1]
Human keratinocytes respond to interleukin-18: implication for the course of chronic inflammatory skin diseases:
M. Wittmann, et al.; J. Invest. Dermatol.
124
, 1225 (2005)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-802-002
Revised 05-Mar-07
Monoclonal Antibody to Nicotinic Acetylcholine Receptor (88B)
SYNONYMS
anti-nAChR MAb (88B)
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Acetylcholine Receptors (Nicotinic) / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-802-002-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
CLONE:
88B
ISOTYPE:
Mouse IgG1
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified
Torpedo californica
AChR (acetylcholine receptor).
SPECIFICITY:
Recognizes the intracellular domain of skeletal muscle nAChR γ and δ subunits in
Torpedo californica
and the δ subunit in mouse, rat, chicken, frog and fish. Detects bands of ~60kDa (γ subunit ) and ~65kDa (δ subunit ) by Western blot. Under non-reducing conditions detects a band of ~130kDa (dimer of δ subunit). Does not cross-react with the α1 and β1 subunits in muscle or the neuronal nAChRs.
APPLICATION:
Immunohistochemistry (frozen sections (1:1'000))
Immunoprecipitation
Western Blot (1:5'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies to cytoplasmic domains of the acetylcholine receptor:
S.C. Froehner, et al.; J. Biol. Chem.
258
, 7112 (1983)
Abstract
;
Full Text
Structure of nicotinic acetylcholine receptors:
A. Karlin; Curr. Opinion Neurobiol.
3
, 299 (1993)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-396
Revised 09-Dec-08
Monoclonal Antibody to Nitric Oxide Synthase (Endothelial) (phoshorylated) (pSer1177) (15E2)
SYNONYMS
anti-eNOS (phosphorylated) (pSer
1177
) MAb (15E2)
anti-NOS III (phosphorylated) (pSer
1177
) MAb (15E2)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
NOS Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-396-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
15E2
ISOTYPE:
Mouse IgG1
CONCENTRATION:
100µg/ml
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.
IMMUNOGEN:
Synthetic peptide corresponding to a portion of phosphorylated human endothelial nitric oxide synthase (eNOS; NOS III).
SPECIFICITY:
Recognizes human Ser
1177
-phosphorylated eNOS (NOS III). Does not cross-react with unphosphorylated eNOS (NOS III) or other unrelated serine-phosphorylated proteins.
APPLICATION:
Western Blot
(0.5µg/ml for HRPO/ECL detection)
Recommended blocking buffer: PBS/Tween 20-based blocking and incubation buffer.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-029
Revised 22-Jun-06
Monoclonal Antibody to Nitric Oxide Synthase (Universal) (NOS-3F7-B11-B5)
SYNONYMS
anti-NOS MAb (NOS-3F7-B11-B5)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
NOS Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-029-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
CLONE:
NOS-3F7-B11-B5
ISOTYPE:
Mouse IgM
FORMULATION:
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Purified neuronal nitric oxide synthase (nNOS; NOS I) from bovine brain.
SPECIFICITY:
Recognizes iNOS (NOS II), eNOS (NOS III) and nNOS (NOS I) in mouse, rat and bovine samples. Detects bands of ~130kDa (representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS)), ~155kDa (in tissues expressing nNOS) and ~140kDa (in tissues expressing eNOS) by Western blot.
APPLICATION:
Immunohistochemistry (frozen sections (1:100))
Western Blot (1:250)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia:
C. Xue, et al.; Am. J. Physiol.
267
, L667 (1994)
Abstract
Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivation:
K.Y. Xu, et al.; J. Biol. Chem.
278
, 41798 (2003)
Abstract
;
Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-416
Revised 11-Sep-08
Monoclonal Antibody to Nitrotyrosine [Affinity Sorbent] (CC22.8C7)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitrotyrosine / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-416-C200
200 µg
403.00 USD
Product Specification
CLONE:
CC22.8C7
ISOTYPE:
Mouse IgG
FORMULATION:
200µg of anti-Nitrotyrosine MAb conjugated to 200µl of protein A agarose. Supplied in 200μl TBS, pH 7.4.
IMMUNOGEN:
Peroxynitrite treated KLH (keyhole limpet hemocyanin).
SPECIFICITY:
Recognizes nitrotyrosine-containing proteins.
APPLICATION:
Immunoprecipitation.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 1 year when stored at +4°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Typical procedure for Immunoprecipitation:
1. Dilute the protein sample (cell lysate, tissues homogenate, plasma, etc.) to ~1mg/ml in TBS or PBS in a
microcentrifuge tube.
2. Add 20-40µl of gel slurry to 0.5-1ml (0.5-1mg protein) and incubate 1-2 hours at room temperature or
4°C overnight with occasional mixing or gentle rocking.
3. Collect the beads by brief centrifugation (<1 minute) in a microcentrifuge. Carefully drain or pipet off the
supernatant and wash the beads 3 times with TBS or PBS.
4. Resuspend the beads in 25-50µl of 1X Laemmli sample buffer and boil for 5 minutes. (Alternatively the
samples can be frozen for later use).
5. Briefly centrifuge the sample to pellet the beads. The supernatant can now be directly used for
SDS-PAGE.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-505
Revised 16-Jan-06
Monoclonal Antibody to 3-Nitrotyrosine (39B6)
SYNONYMS
anti-3NTyr MAb (39B6)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitrotyrosine / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-505-C050
50 µg
98.00 USD
ALX-804-505-C200
200 µg
230.00 USD
Product Specification
CLONE:
39B6
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
0.5mg/ml
FORMULATION:
Liquid. In PBS containing 0.09% sodium azide.
IMMUNOGEN:
3-(4-Hydroxy-3-nitrophenylacetamido) propionic acid-BSA conjugate.
SPECIFICITY:
Recognizes 3-nitrotyrosine moieties. Not species specific.
APPLICATION:
ELISA (with antigen coated plates)
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Western Blot (1:5'000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control see Prod. No.
ALX-840-014
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC
:
I. Girault, et al.; Free Radical Biology & Medicine
31
, 1375 (2001)
Abstract
Inducible Nitric Oxide Synthase and Nitrotyrosine in Listeric Encephalitis: A Cross-species Study in Ruminants
:
H. Pfister, et al.; Vet. Pathology
39
, 190 (2002)
Abstract
;
Full Text
Comparison of nitrotyrosine antibodies and development of immunoassays for the detection of nitrated proteins:
T. Franze, et al.; Analyst
129
, 589 (2004)
Abstract
Related Products
ALX-840-014
Nitrotyrosine-modified BSA (Positive Control for Prod. No.
ALX-804-208
)
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-204
Revised 07-Oct-08
Monoclonal Antibody to Nitrotyrosine (CC22.8C7)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitrotyrosine / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-204-C050
50 µg
126.00 USD
ALX-804-204B-C100
Biotin
100 µg
357.00 USD
ALX-804-204-C200
200 µg
448.00 USD
Product Specification
CLONE:
CC22.8C7
ISOTYPE:
Mouse IgG2b
CONCENTRATION:
FORMULATION:
CC22.8C7:
Liquid. In PBS, pH 7.2, containing 50% glycerol, 0.1mg/ml BSA and 0.02% sodium azide.
CC22.8C7-Biotin:
Liquid. In PBS, pH 7.2, containing 0.5mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:
Peroxynitrite-treated KLH (keyhole limpet hemocyanin).
SPECIFICITY:
Recognizes nitrotyrosine-containing proteins.
APPLICATION:
ELISA [1]
Immunohistochemistry (10µg/ml)
Immunoprecipitation
Western Blot (1-2µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
CC22.8C7:
Stable for at least 2 years after receipt when stored at -20°C.
CC22.8C7-Biotin:
Stable for at least 1 year after receipt when stored at +4°C.
POSITIVE CONTROL:
For Positive Control see Prod. No.
ALX-840-013
.
Product Specific Literature References
[1]
Enzyme immunoassays for the investigation of protein nitration by air pollutants
:
T. Franze, et al.; Analyst.
128
, 824 (2003)
Abstract
General Information
Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by nitric oxide synthase. NO reacts rapidly with superoxide (6.7 x 10
9
/M/sec) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation
in vivo
. Nitrotyrosine has been shown to be present in proteins from a variety of clinical conditions including atherosclerotic lesions of human coronary arteries, postischemic heart, and placenta during preeclampsia. Increased nitration of proteins in motor neurons has been identified in patients with ALS (amyotrophic lateral sclerosis) and may be due to mutations in superoxide dismutase.
General Literature References
Oxidative chemistry of peroxynitrite
:
J.S. Beckman, et al.; Meth. Enzymol.
233
, 229 (1994)
Abstract
Extensive nitration of protein tyrosines in human atherosclerosis detected by immunohistochemistry
:
J.S. Beckman, et al.; Biol. Chem. Hoppe-Seyler
375
, 81 (1994)
Abstract
Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and the ugly
:
J.S. Beckman and W.H. Koppenol; Am. J. Physiol.
271
, C1424 (1996)
Abstract
Measurement of nitric oxide and peroxynitrite generation in the postischemic heart. Evidence for peroxynitrite-mediated reperfusion injury
:
P. Wang and J.L. Zweier; J. Biol. Chem.
271
, 29223 (1996)
Abstract
;
Full Text
Colocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amytrophic lateral sclerosis: An immunohistochemical study
:
S.M. Chou, et al.; J. Chem. Neuroanat.
10
, 249 (1996)
Abstract
Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action
:
L. Myatt, et al.; Hypertension
28
, 488 (1996)
Abstract
Increased 3-nitrotyrosine and oxidative damage in mice with a human copper/zinc superoxide dismutase mutation
:
R.J. Ferrante, et al.; Ann. Neurol.
42
, 326 (1997)
Abstract
Upregulation of protein-tyrosine nitration in the anterior horn cells of amyotrophic lateral sclerosis
:
K. Abe, et al.; Neurol. Res.
19
, 124 (1997)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-208
Revised 25-Nov-08
Monoclonal Antibody to Nitrotyrosine (NOY-7A5)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
Nitrotyrosine / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-208-C100
100 µg
380.00 USD
Product Specification
CLONE:
NOY-7A5
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
KLH (keyhole limpet hemocyanin) modified with nitrotyrosine.
SPECIFICITY:
Recognizes nitrotyrosine-modified proteins.
APPLICATION:
Western Blot
(use 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
For Positive Control see Prod. No.
ALX-840-014
.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-628
Revised 17-Oct-05
Monoclonal Antibody to NKp44 (human) (8F12)
SYNONYMS
anti-Natural Killer Cell p44-related Protein (human) MAb (8F12)
anti-Natural Cytotoxicity Triggering Receptor 2 (human) MAb (8F12)
anti-NK Cell Activating Receptor (human) MAb (8F12)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
NK Cells Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-628-R050
50 µl
195.00 USD
ALX-804-628-R100
100 µl
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
8F12
ISOTYPE:
Mouse IgG3
CONCENTRATION:
1mg/ml
PURITY:
Liquid. In PBS, pH 7.4, containing 0.02% thimerosal.
PURITY DETAIL:
Protein G-affinity purified.
IMMUNOGEN:
Recombinant human NKp44 (natural killer cell p44-related protein) extracellular domain (aa 19-130).
SPECIFICITY:
Recognizes human NKp44. Does not cross-react with human NKp30 and NKp46.
APPLICATION:
ELISA
Western Blot (1:1’00-1:2’000)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 1 month when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Immune Receptors Other Products
•
Monoclonal Antibodies
ALX-804-630
Revised 31-Oct-06
Monoclonal Antibody to KIR2D (human) (2H6)
SYNONYMS
anti-Killer Cell Immunoglobulin-like Receptors 2D (human) MAb (2H6)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Killer Cell Immunoglobulin-like Receptors [KIRs] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-630-R050
50 µl
195.00 USD
ALX-804-630-R100
100 µl
320.00 USD
Product Specification
CLONE:
2H6
ISOTYPE:
Mouse IgG2b
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Liquid. In PBS, pH 7.4, containing 0.02% thimerosal.
IMMUNOGEN:
Recombinant extracellular KIR2DL4 (killer cell immunoglobulin-like receptor 2DL4).
SPECIFICITY:
Recognizes human KIR2DL1, KIR2DL3, KIR2DL4 and KIR2DS4.
APPLICATION:
ELISA
Western Blot (1:500-1:2’000)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 month when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-629
Revised 31-Oct-06
Monoclonal Antibody to NKp30 (human) (y4E6)
SYNONYMS
anti-Natural Killer Cell p30-related Protein (human) MAb (y4E6)
anti-Natural Cytotoxicity Triggering Receptor 3 (human) MAb (y4E6)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
NK Cell Markers / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-629-R050
