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Items 9 of 9
ALX-850-059
Revised 17-Apr-08
VE-cadherin, Soluble ELISA Kit
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Kits & Sets
Ordering Information
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ALX-850-059-KI01
1 Kit
619.00 USD
Product Specification
SENSITIVITY:
0.15ng/ml (range 0.16 to 10ng/ml)
QUANTITY:
96 wells (~80 tests).
APPLICATION:
For the quantitative determination of soluble human VE-cadherin in cell culture supernatants, serum and other body fluids.
SHIPPING:
SHIPPED ON BLUE ICE
HANDLING:
Store standard and biotin-conjugate at -20°C. Store rest of the kit at +4°C.
General Information
MANUFACTURER
Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits
ALX-804-393
Revised 31-Oct-06
Monoclonal Antibody to M-cadherin (mouse) (12G4)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
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Format:
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ALX-804-393-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
12G4
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:
Recombinant mouse M-cadherin extracellular domain.
SPECIFICITY:
Recognizes mouse M-cadherin.
APPLICATION:
Immunocytochemistry
(5µg/ml)
Western Blot
(1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
General Information
Cadherins are a family of transmembrane glycoproteins that play a key role in calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. M-Cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-202
Revised 23-Sep-08
Monoclonal Antibody to E-cadherin (ECCD-2)
SYNONYMS
anti-Uvomorulin MAb (ECCD-2)
anti-CD324 MAb (ECCD-2)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-202-C100
100 µg
390.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
CLONE:
ECCD-2
ISOTYPE:
Rat IgG2a
SOURCE/HOST:
Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment.
CONCENTRATION:
2mg/ml after reconstitution.
PURITY DETAIL:
Purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography.
FORMULATION:
Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl
2
, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN:
Mouse liver E-cadherin fragment.
SPECIFICITY:
Recognizes human, mouse and dog E-cadherin. Does not cross-react with bovine or chicken E-cadherin.
APPLICATION:
Immunohistochemistry
(frozen sections (10µg/ml), not recommended for paraffin sections)
Western Blot
(10µg/ml)
Functional Application
Does not inhibit E-cadherin dependent cell-cell contact and can be used to analyze the mechanism.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin:
Y. Shirayoshi, et al.; Cell Struct. Funct.
11
, 245 (1986)
Abstract
Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA:
A. Nagafuchi, et al.; Nature
329
, 341 (1987)
Abstract
The cadherins: cell-cell adhesion molecules controlling animal morphogenesis:
M. Takeichi; Development
102
, 639 (1988)
Abstract
Cadherin cell adhesion receptors as a morphogenetic regulator:
M. Takeichi; Science
251
, 1451 (1991)
Abstract
Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7):
P.I. Karecla, et al.; Eur. J. Immunol.
25
, 852 (1995)
Abstract
Role of IQGAP1, a target of the small GTPase Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion:
S. Kuroda, et al.; Science
281
, 832 (1998)
Abstract
The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells:
S. Wallis, et al.; Mol. Biol. Cell.
11
, 1077 (2000)
Abstract
Recruitment and activation of Rac1 by the formation of E-cadherin- mediated cell-cell adhesion sites:
M. Nakagawa, et al.; J. Cell. Sci.
114
, 1829 (2001)
Abstract
Inverse correlation between the extent of N-glycan branching and intercellular adhesion in epithelia. Contribution of the Na,K-ATPase beta1 subunit:
O. Vagin, et al.; J. Biol. Chem.
283
, 2192 (2008)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Buffers should contain Ca
2+
to stabilize cadherin antigen. In case of immunohistochemical staining of acetone fixed frozen sections, Ca
2+
is not necessary.
Related Products
ALX-211-052
Polyclonal Antibody to Rat IgG (Mouse Ig adsorbed) (HRP)
ALX-211-051
Polyclonal Antibody to Rat IgG (Mouse Ig adsorbed) (FITC)
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-804-201
Revised 11-Dec-08
Monoclonal Antibody to E-cadherin (HECD-1)
SYNONYMS
anti-Uvomorulin MAb (HECD-1)
anti-CD324 MAb (HECD-1)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-201-C100
100 µg
390.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE:
HECD-1
ISOTYPE:
Mouse IgG1
SOURCE/HOST:
Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7.
CONCENTRATION:
2mg/ml after reconstitution.
PURITY DETAIL:
Ammonium sulfate precipitation and ion exchange chromatography.
FORMULATION:
Lyophilized from 10mM PBS, pH 7.4 , containing 1% BSA. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl
2
, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN:
Human breast tumor cell line MCF-7.
SPECIFICITY:
Recognizes human and guinea pig E-cadherin. Does not cross-react with rat, dog, bovine or chicken E-cadherin.
APPLICATION:
ELISA (2-10µg/ml)
Flow Cytometry (1µg/ml)
Immunocytochemistry (1µg/ml)
Immunohistochemistry (frozen sections (2-10µg/ml), paraffin sections (2-10µg/ml))
Western Blot (2-10µg/ml)
Functional Application (100µg/ml for inhibition of E-cadherin dependent cell-cell contact)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas:
Y. Shimoyama, et al.; Cancer Res.
49
, 2128 (1989)
Abstract
Expression of immunoreactive E-cadherin adhesion molecules in human cancers:
H. Shiozaki, et al.; Am. J. Pathol.
139
, 17 (1991)
Abstract
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections:
S.R. Shi, et al.; J. Histochem. Cytochem.
39
, 741 (1991)
Abstract
Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma:
Y. Shimoyama & S. Hirohashi; Cancer Lett.
57
, 131 (1991)
Abstract
Expression of E- and P-cadherin in gastric carcinomas:
Y. Shimoyama & S. Hirohashi; Cancer Res.
51
, 2185 (1991)
Abstract
Cadherin cell adhesion receptors as a morphogenetic regulator:
M. Takeichi; Science
251
, 1451 (1991)
Abstract
Immunohistochemical localization of cell adhesion molecule epithelial cadherin in human arachnoid villi and meningiomas:
Y. Tohma, et al.; Cancer Res.
52
, 1981 (1992)
Abstract
J.A. Jankowski, et al.; Int. J. Oncol.
4
, 441 (1994)
CD123bright plasmacytoid predendritic cells: progenitors undergoing cell fate conversion?:
M.R. Comeau, et al.; J. Immunol.
169
, 75 (2002)
Abstract
;
Full Text
Changing etiology of tubal pregnancy following IVF:
A. Revel, et al.; Hum. Reprod.
6
, 1372 (2008)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Ca
2+
should be contained in all buffers to stabilize cadherin antigen.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-804-203
Revised 06-Mar-08
Monoclonal Antibody to E-cadherin (human) (SHE78-7)
SYNONYMS
anti-Uvomorulin (human) MAb (SHE78-7)
anti-CD324 (human) MAb (SHE78-7)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
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ALX-804-203-C100
100 µg
390.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
SHE78-7
ISOTYPE:
Mouse IgG2a
SOURCE/HOST:
Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with soluble E-cadherin from human placenta.
CONCENTRATION:
2mg/ml after reconstitution.
PURITY DETAIL:
Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography.
FORMULATION:
Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservatives.
RECONSTITUTION:
Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl
2
, 1% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
Note:
TBS should be used for dilution, as PBS causes precipitation.
IMMUNOGEN:
Soluble E-cadherin from human placenta.
SPECIFICITY:
Recognizes human E-cadherin. Does not cross-react with rat E-cadherin.
APPLICATION:
ELISA
(2µg/ml)
Flow Cytometry
(1µg/ml)
Immunohistochemistry
(frozen sections (2µg/ml), paraffin sections (2µg/ml); good results were obtained by incubating with the primary antibody at 4°C overnight);
Western Blot
(2µg/ml; reducing and non-reducing conditions);
Functional Application
(1µg/ml for inhibition of E-cadherin-dependent cell-cell contact)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Soluble E-cadherin fragments increased in circulation of cancer patients
:
M. Katayama, et al.; Br. J. Cancer
69
, 580 (1994)
Abstract
Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line
:
M. Watabe, et al.; J. Cell. Biol.
127
, 247 (1994)
Abstract
;
Full Text
Soluble fragment of E-cadherin cell adhesion molecule increase in urinary excretion of cancer patients, potentially indicating its shedding from epithelial tumour cells:
M. Katayama, et al.; Int. J. Oncol.
5
, 1049 (1994)
General Information
BACKGROUND/TECHNICAL INFORMATION
Ca
2+
should be contained in the buffers to stabilize cadherin antigen. In case of immunohistochemical staining of paraffin embedded tissues and acetone fixed frozen sections, Ca
2+
is not necessary.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-804-263
Revised 18-Aug-05
Monoclonal Antibody to E-cadherin (mouse) (ECCD-1)
SYNONYMS
anti-Uvomorulin (mouse) MAb (ECCD-1)
anti-CD324 (mouse) MAb (ECCD-1)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-804-263-C100
100 µg
390.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE:
ECCD-1
ISOTYPE:
Rat IgG2b
SOURCE/HOST:
Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of Wistar rat immunized with mouse teratocarcinoma cell line F9. Purified from serum-free cell culture supernatant.
CONCENTRATION:
2mg/ml after reconstitiution.
FORMULATION:
Lyophilized from ascites, dissolved in 10mM PBS, pH 7.4, containing 1.0% BSA.
RECONSTITUTION:
Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl
2
, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN:
Mouse teratocarcinoma cell line F9.
SPECIFICITY:
Recognizes mouse E-cadherin. Epitope is close to the N-terminus of E-cadherin. Does not cross-react with human, bovine, chicken or dog E-cadherin.
APPLICATION:
Functional Application
: Inhibits E-cadherin-dependent cell-cell contact (200µg/ml).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Specific Literature References
Molecular nature of the calcium-dependent cell-cell adhesion system in mouse teratocarcinoma and embryonic cells studied with a monoclonal antibody:
C. Yoshida-Noro, et al.; Dev. Biol.
101
, 19 (1984)
Abstract
Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA:
A. Nagafuchi, et al.; Nature
329
, 341 (1987)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Ca
2+
should be contained in all the buffers to stabilize cadherin antigen.
Further Categories Containing This Product:
Monoclonal Antibodies
•
CD Antibodies
ALX-803-305
Revised 07-Nov-08
Monoclonal Antibody to VE-cadherin (human) (BV6)
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-803-305-C100
100 µg
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
BV6
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
0.1mg/ml
PURITY:
Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
PURITY DETAIL:
Affinity purified.
IMMUNOGEN:
Human endothelial cells.
SPECIFICITY:
Recognizes human VE-cadherin. Does not cross-react with other species. Detects a band of ~130-140kDa by Western blot.
APPLICATION:
ELISA
Flow Cytometry
Immunocytochemistry (fluorescence and peroxidase staining)
Immunohistochemistry (frozen section)
Immunoprecipitation
Western Blot (non-reducing)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Functional properties of human vascular endothelial cadherin (7B4/cadherin-5), an endothelium-specific cadherin:
F. Breviario, et al.; Arterioscler. Thromb. Vasc. Biol.
15
, 1229 (1995)
Abstract
Catenin-dependent and -independent functions of vascular endothelial cadherin:
P. Navarro, et al.; J. Biol. Chem.
270
, 30965 (1995)
Abstract
;
Full Text
Inhibition of cultured cell growth by vascular endothelial cadherin (cadherin-5/VE-cadherin):
L. Caveda, et al.; J. Clin. Invest.
98
, 886 (1996)
Abstract
;
Full Text
Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization:
P. Navarro, et al.; J. Cell Biol.
140
, 1475 (1998)
Abstract
;
Full Text
Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization:
T. Murohara, et al.; J. Clin. Invest.
105
, 1527 (2000)
Abstract
;
Full Text
Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability:
M. Corada, et al.; Blood
97
, 1679 (2001)
Abstract
;
Full Text
VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells:
P. Salven, et al.; Blood
101
, 168 (2003)
Abstract
;
Full Text
Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells:
K. Xiao, et al.; J. Cell Biol.
163
, 535 (2003)
Abstract
;
Full Text
p120-Catenin regulates clathrin-dependent endocytosis of VE-cadherin:
K. Xiao, et al.; Mol. Biol. Cell
16
, 5141 (2005)
Abstract
;
Full Text
Further Categories Containing This Product:
Angiogenesis Other Products
•
Angiogenesis Other Products
•
Monoclonal Antibodies
ALX-210-618
Revised 28-Aug-08
Polyclonal Antibody to N-cadherin
SYNONYMS
anti-CD325 PAb
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-618-C400
400 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
2mg/ml after reconstitution.
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with 200µl distilled water. Dilute with 20mM TBS, pH 7.4, containing 1% BSA and 0.1% sodium azide and 10mM calcium chloride.
IMMUNOGEN:
Synthetic peptide corresponding to aa 808-827 of human N-cadherin.
SPECIFICITY:
Recognizes human, mouse, bovine and chicken N-cadherin. Does not cross-react with human E-or P-cadherin.
APPLICATION:
Immunohistochemistry (frozen and paraffin sections (5-10μg/ml))
Western Blot (5-10μg/ml)
Optimal conditions must be determined individually for each application.
Note: Does not inhibit N-cadherin dependent cell-cell contact.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 6 months when stored at +4°C and for 1 year when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
TBS should be used for dilution as PBS may cause precipitation. In case of immunohistochemistry on paraffin or frozen sections, Ca
2+
is not necessary.
Further Categories Containing This Product:
Polyclonal Antibodies
•
CD Antibodies
ALX-210-232
Revised 06-Jan-09
Polyclonal Antibody to VE-cadherin
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-232-C100
Purified
100 µg
308.00 USD
ALX-210-232B-T100
Biotin
100 tests
355.00 USD
ALX-210-232F-T100
FITC
100 tests
355.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Affinity purified using recombinant VE-cadherin fragments.
FORMULATION:
Unconjugated PAb
: Liquid. 1mg/ml in PBS.
Biotin- and FITC-conjugates:
Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
IMMUNOGEN:
Recombinant human VE-cadherin (aa 1-258).
SPECIFICITY:
Recognizes human, mouse, rat and bovine VE-cadherin.
APPLICATION:
Confocal Microscopy
[1]
ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry:
Frozen sections (formalin or acetone-fixed sections) (1µg/ml); not recommended for paraffin sections.
Western Blot
[1] (see also protocol modifications)
Functional Application:
Interferes with VE-cadherin adhesion (reversible endothelium disruption) This effect mimics the transmigration of leukocytes or cancer cells through endothelium.
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
HANDLING:
For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
[1]
Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin:
D. Gulino, et al.; J. Biol. Chem.
273
, 29786 (1998)
Abstract
;
Full Text
VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function:
S. Iyer, et al.; Am. J. Physiol. Lung Cell Mol. Physiol.
286
, L1143 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Protocol Modifications for Western blot:
Protocol described in [1]; To improve the detection of VE cadherin, the following modifications are recommended
:
Use PAb to VE-cadherin (Prod. No. ALX-210-232) diluted to 1µg/ml in PBS containing 0.01% Thimerosal (concentration must be adjusted according to the batch used). The solution can be used several times. Incubate overnight at room temperature under stirring. Rinsing: 6 x 5 minutes plus 10 minutes at room temperature.
Secondary antibody
: Biotinylated goat anti-rabbit antibody (VC-BA-1000) diluted at 1:5’000 in 1% gelatin. Avoid exposure to light. Incubation 1h 30 at room temperature under stirring. Rinse in PBS 0.05% Tween 20 for 50 minutes at room temperature under stirring.
Signal amplification using Strept ABComplex/ HRP (Dako)
: Follow the protocol according to Dako instructions. Dilute Strept ABComplex/HRP to 1:5’000. Avoid exposure to light. Incubate 1h at room temperature while stirring. Rinse in PBS 0.05% Tween 20, 50 minutes at room temperature while stirring. Rinse rapidly in PBS.
Detection using the Supersignal Kit Pierce
: Incubate the membrane 10 minutes with the solution under red light. Membrane is then packed in Plastic wrap and put in contact with the film. This method should allow for the detection of very small amounts of VE cadherin (less than 1ng/well).
Further Categories Containing This Product:
Angiogenesis Other Products
•
Polyclonal Antibodies
