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ALX-270-263 Revised 03-Apr-08
(-)-Epigallocatechin gallate
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SYNONYMS EGCG
(2R,3R)-2-(3,4,5-Trihydroxyphenyl)-3,4-dihydro-1[2H]-benzopyran-3,5,7-triol 3-(3,4,5-trihydroxybenzoate)
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Antioxidants
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-270-263-M010   10 mg 20.00 USD Add To Cart
ALX-270-263-M050   50 mg 60.00 USD Add To Cart
Product Specification
FORMULA: C22H18O11
MW: 458.4
CAS NUMBER: 989-51-5
MERCK INDEX: 14: 3526
SOURCE/HOST: Isolated from green tea.
PURITY: ≥98% (HPLC)
APPEARANCE: Off-white to yellow powder.
SOLUBILITY: Soluble in 100% ethanol, dimethyl formamide or DMSO; slightly soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Stock solutions in DMSO are stable for up to 3 months when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Keep under inert gas. Protect from light.

Product Description
Antitumor reagent. Antioxidant. Protects cells from lipid peroxidation and DNA damage induced by reactive free radicals. Inhibits inducible nitric oxide synthase (iNOS; NOS II). Chemopreventive anticancer agent. Induces apoptosis in human cancer cell lines. Inhibits MAP kinase mediated signalling pathways. Inhibits angiogenesis. Inhibits telomerase and DNA methyltransferase. Anti-inflammatory agent.
Product Specific Literature References
Inhibitory effect of topical application of a green tea polyphenol fraction on tumor initiation and promotion in mouse skin: M.T. Huang, et al.; Carcinogenesis 13, 947 (1992) Abstract
Inhibition of 12-O-tetradecanoylphorbol-13-acetate and other skin tumor-promoter-caused induction of epidermal interleukin-1 alpha mRNA and protein expression in SENCAR mice by green tea polyphenols: S.K. Katiyar, et al.; J. Invest. Dermatol. 105, 394 (1995) Abstract
Growth inhibition and regression of human prostate and breast tumors in athymic mice by tea epigallocatechin gallate: S. Liao, et al.; Cancer Lett. 96, 239 (1995) Abstract
Inhibition of N-methyl-N'-nitro-N-nitrosoguanidine-induced carcinogenesis by (-)-epigallocatechin gallate in the rat glandular stomach: T. Yamane, et al.; Cancer Res. 55, 2081 (1995) Abstract
(-)-Epigallocatechin gallate, a polyphenolic tea antioxidant, inhibits peroxynitrite-mediated formation of 8-oxodeoxyguanosine and 3-nitrotyrosine: E.S. Fiala, et al.; Experientia 52, 922 (1996) Abstract
Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells: N. Ahmad, et al.; J. Natl. Cancer Inst. 89, 1881 (1997) Abstract
Inhibition of inducible nitric oxide synthase gene expression and enzyme activity by epigallocatechin gallate, a natural product from green tea: M.M. Chan, et al.; Biochem. Pharmacol. 54, 1281 (1997) Abstract
(-)-Epigallocatechin-3-gallate blocks the induction of nitric oxide synthase by down-regulating lipopolysaccharide-induced activity of transcription factor nuclear factor-kappaB: Y.L. Lin & J.K. Lin; Mol. Pharmacol. 52, 465 (1997) Abstract
Telomerase inhibition, telomere shortening, and senescence of cancer cells by tea catechins: I. Naasani, et al.; BBRC 249, 391 (1998) Abstract
Epigallocatechin suppression of proliferation of vascular smooth muscle cells: correlation with c-jun and JNK: L.H. Lu, et al.; Br. J. Pharmacol. 124, 1227 (1998) Abstract
ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers: Q. Guo, et al.; Biochim. Biophys. Acta 1427, 13 (1999) Abstract
Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin: K. Dvorakova, et al.; Cancer Chemother. Pharmacol. 43, 331 (1999) Abstract
Polyphenolic antioxidant (-)-epigallocatechin-3-gallate from green tea reduces UVB-induced inflammatory responses and infiltration of leukocytes in human skin: S.K. Katiyar, et al.; Photochem. Photobiol. 69, 148 (1999) Abstract
Antioxidant chemistry of green tea catechins. Identification of products of the reaction of (-)-epigallocatechin gallate with peroxyl radicals: S. Valcic, et al.; Chem. Res. Toxicol. 12, 382 (1999) Abstract
Antimutagenic and anticarcinogenic activity of tea polyphenols: Y. Kuroda & Y. Hara; Mutat. Res. 436, 69 (1999) Abstract
Inhibitory effect of green and black tea on tumor growth: A.H. Conney, et al.; Proc. Soc. Exp. Biol. Med. 220, 229 (1999) Abstract
Green tea epigallocatechin gallate: a natural inhibitor of fatty-acid synthase: X. Wang & W. Tian; BBRC 288, 1200 (2001) Abstract
Epigallocatechin gallate, a constituent of green tea, represses hepatic glucose production: M.E. Waltner-Law, et al.; J. Biol. Chem. 277, 34933 (2002) Abstract; Full Text
The specificities of protein kinase inhibitors: an update:: J. Bain, et al.; Biochem. J. 371, 199 (2003) Abstract; Full Text
Selective inhibition of ADAMTS-1, -4 and -5 by catechin gallate esters: M.N. Vankemmelbeke, et al.; Eur. J. Biochem. 270, 2394 (2003) Abstract
Epigallocatechin-3-gallate, constituent of green tea, suppresses the LPS-induced phenotypic and functional maturation of murine dendritic cells through inhibition of mitogen-activated protein kinases and NF-kappaB: S.C. Ahn, et al.; BBRC 313, 148 (2004) Abstract
Epigallocatechin-3-gallate inhibits epidermal growth factor receptor signaling pathway. Evidence for direct inhibition of ERK1/2 and AKT kinases: J.F. Sah, et al.; J. Biol. Chem. 279, 12755 (2004) Abstract
Inhibition of c-Jun NH2-terminal kinase activity improves ischemia/reperfusion injury in rat lungs: M. Ishii, et al.; J. Immunol. 172, 2569 (2004) Abstract
A constituent of green tea, epigallocatechin-3-gallate, activates endothelial nitric oxide synthase by a phosphatidylinositol-3-OH-kinase-, cAMP-dependent protein kinase-, and Akt-dependent pathway and leads to endothelial-dependent vasorelaxation: M. Lorenz, et al.; J. Biol. Chem. 279, 6190 (2004) Abstract; Full Text
Green tea polyphenols prevent toxin-induced hepatotoxicity in mice by down-regulating inducible nitric oxide-derived prooxidants: J.H. Chen, et al.; Am. J. Clin. Nutr. 80, 742 (2004) Abstract; Full Text
Neuroprotective effects of (-)-epigallocatechin gallate following hypoxia-ischemia-induced brain damage: novel mechanisms of action: B.A. Sutherland, et al.; FASEB J. 19, 258 (2005) Abstract; Full Text
Tea flavanols inhibit angiotensin-converting enzyme activity and increase nitric oxide production in human endothelial cells: I.A. Persson, et al.; J. Pharm. Pharmacol. 58, 1139 (2006) Abstract
General Literature References
Anticarcinogenic effects of (-)-epigallocatechin gallate: H. Fujiki, et al.; Prev. Med. 21, 503 (1992), (Review) Abstract
Absorption, metabolism and antioxidative effects of tea catechin in humans: T. Miyazawa; Biofactors 13, 55 (2000), (Review) Abstract
Inhibition of tumour invasion and angiogenesis by epigallocatechin gallate (EGCG), a major component of green tea: Y.D. Jung and L.M. Ellis; Int. J. Exp. Pathol. 82, 309 (2001), (Review) Abstract
Anti-invasive effects of green tea polyphenol epigallocatechin-3-gallate (EGCG), a natural inhibitor of metallo and serine proteases: R. Benelli, et al.; Biol. Chem. 383, 101 (2002), (Review) Abstract
Further Categories Containing This Product:
Flavanols • PKC Inhibitors • MAPK Pathway Inhibitors • Natural Products - Chemopreventive Agents • Fatty Acid Synthase [FAS] / Related Products • Active Substances from Fruit and Vegetables • Akt [PKB] / Related Products • NOS Inhibitors (NOS Induction & Enzyme Activity) • Natural Products for Angiogenesis Research • JNK [SAPK1] / Related Products • Natural Products - Protein Kinase Inhibitors • Natural Products - Anti-inflammatory Agents • Antitumor Agents (Enzyme Inhibitors) • EGFR Kinase Inhibitors • Telomerase / Related Products • Natural Products - Antitumor Reagents • Antitumor Agents (DNA Interaction & Gene Regulation) • Antitumor Agents (Apoptosis Inducers) • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Nitric Oxide Pathway Modulators
 
 
ALX-350-017 Revised 18-Nov-08
Fumonisin B1
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Tumor Promoters
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-017-M001   1 mg 60.00 USD Add To Cart
ALX-350-017-M005   5 mg 245.00 USD Add To Cart
Product Specification
FORMULA: C34H59NO15
MW: 721.8
CAS NUMBER: 116355-83-0
MERCK INDEX: 14: 4289
RTECS: TZ8350000
SOURCE/HOST: Isolated from Fusarium moniliforme.
PURITY: ~98% (TLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in water (10mg/ml), methanol (5mg/ml), DMSO (20mg/ml) or acetonitrile.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: MAY BE MUTAGENIC. MAY BE TERATOGENIC. MAY BE CARCINOGENIC. HARMFUL.

Product Description
Mycotoxin. Induces DNA damage in the liver and cancer. Inhibitor of sphingosine biosynthesis. Induces apoptosis. Activates Akt (protein kinase B; PKB), which leads to increased survival, inhibition of GSK-3β activity and post-translational stabilization of cyclin D1.
Product Specific Literature References
Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme: E. Wang, et al.; J. Biol. Chem. 266, 14486 (1991) Abstract; Full Text
Fumonisin B1 inhibits sphingosine (sphinganine) N-acyltransferase and de novo sphingolipid biosynthesis in cultured neurons in situ: A.H. Merrill Jr., et al.; J. Biol. Chem. 268, 27299 (1993) Abstract; Full Text
Biological activities of fumonisins, mycotoxins from Fusarium moniliforme, in jimsonweed (Datura stramonium L.) and mammalian cell cultures: H.K. Abbas, et al.; Toxicon 31, 345 (1993) Abstract
Fumonisins and Alternaria alternata lycopersici toxins: sphinganine analog mycotoxins induce apoptosis in monkey kidney cells: H. Wang, et al.; PNAS 93, 3461 (1996) Abstract
A potential mechanism for fumonisin B(1)-mediated hepatocarcinogenesis: cyclin D1 stabilization associated with activation of Akt and inhibition of GSK-3beta activity: D. Ramljak, et al.; Carcinogenesis 21, 1537 (2000) Abstract
Implications of apoptosis for toxicity, carcinogenicity, and risk assessment: fumonisin B(1) as an example: Y.P. Dragan, et al.; Toxicol. Sci. 61, 6 (2001), Review Abstract
Fumonisin B(1): oxidative status and DNA damage in rats: A.M. Domijan, et al.; Toxicology 232, 163 (2007) Abstract
Further Categories Containing This Product:
Akt [PKB] / Related Products • Carcinogens & Tumor Promoters Other Products • DNA Damage & Repair Other Products • Mycotoxins • Sphingolipids & Glycosphingolipids Other Products • Natural Products - Apoptosis Inducers & Inhibitors
 
 
ALX-270-292 Revised 09-Feb-05
1L-6-Hydroxymethyl-chiro-inositol-2-[(R)-2-O-methyl-3-O-octadecylcarbonate]
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Phosphoinositide 3-kinase [PI(3)K] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-270-292-M001   1 mg 85.00 USD Add To Cart
ALX-270-292-M005   5 mg 330.00 USD Add To Cart
Product Specification
FORMULA: C30H58O10
MW: 578.8
PURITY: >98%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Keep cool and dry.

Product Description
Potent inhibitor of Akt (PKB) (IC50=2.0µM). Weak inhibitor of PI(3)K (phosphoinositide 3-kinase) (IC50=83µM). Has been shown to inhibit the growth of the following cancer cell lines: HeLa (IC50=2.5µM), HT-29 (IC50=10µM), MCF-7 (IC50=1.2µM) and PC-3 (IC50=2.0µM).
Product Specific Literature References
3-(Hydroxymethyl)-bearing phosphatidylinositol ether lipid analogues and carbonate surrogates block PI3-K, Akt, and cancer cell growth: Y. Hu, et al.; J. Med. Chem. 43, 3045 (2000) Abstract
Further Categories Containing This Product:
Akt [PKB] / Related Products • Antitumor Agents (Enzyme Inhibitors) • Protein Kinase Inhibitors Other Products
 
 
ALX-804-400 Revised 20-Sep-07
Monoclonal Antibody to Akt (dephosphorylated) (11A11)
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SYNONYMS anti-PKB (dephosphorylated) MAb (11A11)
anti-Protein Kinase B (dephosphorylated) MAb (11A11)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-400-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 11A11
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal (QFSYSA) of human Akt (protein Kinase B; PKB) dephosphorylated at Ser472/473/474.
SPECIFICITY: Recognizes human, mouse, rat and dog Ser472/473/474-dephosphorylated Akt (see technical note). Does not cross-react with Ser472/473/474-phosphorylated Akt.
APPLICATION: Immunohistochemistry
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:

Included (Prod. No. ALX-840-030).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% aa identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. The phosphorylation site slightly differs, thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2:  L461LELDQRTHFPQFS474YSASIRE481        Swiss-Prot-Link: P31751
Akt3:  D461NERRPHFPQFS472YSASGRE479         Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-553 Revised 01-Dec-05
Monoclonal Antibody to Akt (5C10)
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SYNONYMS anti-PKB MAb (5C10)
anti-Protein Kinase B MAb (5C10)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-553-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 5C10
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal human Akt (protein kinase B; PKB).
SPECIFICITY: Recognizes a c-terminal epitope (aa 466-480) of phosphorylated and non-phosphorylated human, mouse, rat and dog Akt isoforms (see Technical Note below).
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-030).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBa), Akt2 (PKBb), and Akt3 (PKBg). Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms.Thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3 respectively.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2: L461LELDQRTHFPQFS474YSASIRE481         Swiss-Prot-Link: P31751
Akt3:     D461NERRPHFPQFS472YSASGRE479      Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-803-303 Revised 18-Sep-07
Monoclonal Antibody to Akt (19G7)
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SYNONYMS anti-PKB MAb (19G7)
anti-Protein Kinase B MAb (19G7)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-803-303-C100   100 µg 240.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 19G7
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.2mg/ml
PURITY: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
PURITY DETAIL: Protein A-affinity purified.
IMMUNOGEN: Recombinant mouse Akt (protein kinase B; PKB).
SPECIFICITY: Recognizes human, mouse, rat, dog and hamster Akt. Detects Akt1 (PKB-α) and Akt2 (PKB-β) strong than Akt3 (PKB-γ). Detects also phosphorylated forms of Akt.
APPLICATION: ELISA
Immunoprecipitation (5µg per 700µl protein extract)
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-634 Revised 05-Jun-07
Monoclonal Antibody to Akt2 (human) (8B7)
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SYNONYMS anti-PKBβ MAb (human) (8B7)
anti-Protein Kinase Bβ MAb (human) (8B7)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-634-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 8B7
ISOTYPE: Mouse IgG2a
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to human Akt2 (Protein Kinase Bβ; PKBβ).
SPECIFICITY: Recognizes human Akt2 (see Technical Note below).
APPLICATION: Immunohistochemistry
Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL: Included (Prod. No. ALX-840-030).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% amino acid identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is the most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. In particular, Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. Note that the phosphorylation site slightly differs between the three isoforms.Thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3 respectively.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2:  L461LELDQRTHFPQFS474YSASIRE481        Swiss-Prot-Link: P31751
Akt3:  D461NERRPHFPQFS472YSASGRE479         Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-401 Revised 28-Mar-08
Monoclonal Antibody to Akt (phosphorylated) (pSer473) (11E6)
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SYNONYMS anti-PKB (phosphorylated) (pSer473) MAb (11E6)
anti-Protein Kinase B (phosphorylated) (pSer473) MAb (11E6)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Akt [PKB] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-401-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 11E6
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to C-terminal (PQFpSYSA) of human Akt (protein kinase B; PKB) phosphorylated at Ser473.
SPECIFICITY: Recognizes human, mouse, rat and dog Ser472/473/474-phosphorylated Akt (see technical note). Does not cross-react with dephosphorylated Akt or other unrelated serine-phosphorylated proteins.
APPLICATION: Flow Cytometry
Immunohistochemistry
Western Blot: (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-027).
General Information
BACKGROUND/TECHNICAL INFORMATION Technical Note: There are three mammalian isoforms of Akt: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 shares 81% and 83% aa identity with Akt2 and Akt3, respectively. Although the three isoforms show broad tissue distribution, Akt1 is most ubiquitously expressed. Akt2 is expressed at a lower level than Akt1 except in insulin-responsive tissues where it predominates. Akt2 is more abundant and more highly activated than Akt1 in adipocytes. Akt3 is expressed at the lowest level except in testes and brain. The C-terminal sequence of the three isoforms are shown below. The phosphorylation site slightly differs, thus the phosphorylation site Ser473 of Akt1 corresponds to Ser474 in Akt2 and Ser472 in Akt3.

Akt1:  V461DSERRPHFPQFS473YSASSTA480       Swiss-Prot-Link: P31749
Akt2:  L461LELDQRTHFPQFS474YSASIRE481       Swiss-Prot-Link: P31751
Akt3:  D461NERRPHFPQFS472YSASGRE479        Swiss-Prot-Link: Q9Y243
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-633 Revised 05-Jun-07
Monoclonal Antibody to Akt1 (5G12)