ALEXIS Biochemicals: Monoclonal Antibody to ERK2 (6G11)

Advanced Search

You are here: Product Lines > Signal Transduction > MAP Kinase Pathways > ERK1 & ERK2 / Related Products

ALX-385-032

Revised 16-Jun-08

(R,S)-Equol
SYNONYMS	(±)-Equol 3,4-Dihydro-3-(4-hydroxyphenyl)-2H-1-benzopyran-7-ol 4’,7-Dihydroxyisoflavane
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Isoflavones

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-385-032-M005		5 mg	30.00 USD
ALX-385-032-M025		25 mg	120.00 USD

Product Specification

FORMULA:	C₁₅H₁₄O₃
MW:	242.3
CAS NUMBER:	531-95-3, 94105-90-5
MERCK INDEX:	14: 3644
PURITY:	≥98% (HPLC)
APPEARANCE:	White to light yellow powder.
SOLUBILITY:	Soluble in DMSO, methanol or 100% ethanol; insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Flavonoid. Racemic mixture. Urinary metabolite of daidzein. Inhibits 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neoplastic cell transformation by targeting the MEK/ERK/p90RSK/activator protein-1 signalling pathway. Shows positive effects on the incidence of prostate cancer and physiological changes after menopause. Functions as a DHT blocker. Preferentially activates estrogen receptor β (ERβ).

Product Specific Literature References

Comparisons of percent equol producers between prostate cancer patients and controls: case-controlled studies of isoflavones in Japanese, Korean and American residents: H. Akaza, et al.; Jpn. J. Clin. Oncol. 34, 86 (2004) Abstract; Full Text
Mammographic density in relation to daidzein-metabolizing phenotypes in overweight, postmenopausal women: C.L. Frankenfeld, et al.; Cancer Epidemiol. Biomarkers Prev. 13, 1156 (2004) Abstract; Full Text
Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback: T.D. Lund, et al.; Biol. Reprod. 70, 1188 (2004) Abstract; Full Text
Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor alpha (ERalpha) and ERbeta in human cells: S.O. Mueller, et al.; Toxicol. Sci. 80, 14 (2004) Abstract; Full Text
High concordance of daidzein-metabolizing phenotypes in individuals measured 1 to 3 years apart: C.L. Frankenfeld, et al.; Br. J. Nutr. 94, 873 (2005) Abstract
Equol, a metabolite of the soybean isoflavone daidzein, inhibits neoplastic cell transformation by targeting the MEK/ERK/p90RSK/activator protein-1 pathway: N.J. Kang, et al.; J. Biol. Chem. 282, 32856 (2007) Abstract; Full Text
Isoflavones, equol and cardiovascular disease: pharmacological and therapeutic insights: K.A. Jackman, et al.; Curr. Med. Chem. 14, 2824 (2007) Abstract

Further Categories Containing This Product:

Estrogen & Estrogen Receptors / Related Products • Antitumor Agents (Hormone-related) • Androgens & Androgen Receptors / Related Products • ERK1 & ERK2 / Related Products

ALX-804-061

Revised 24-Jun-08

Monoclonal Antibody to ERK1/2 (phosphorylated) (12D4)
SYNONYMS	anti-MAPK2/3 (phosphorylated) MAb (12D4) anti-MAP Kinase 2/3 (phosphorylated) MAb (12D4)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	ERK1 & ERK2 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-061-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog
CLONE:	12D4
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2.
SPECIFICITY:	Recognizes the pThr-Glu-pTyr motif of activated human, mouse, rat and dog ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38.
APPLICATION:	Immunocytochemistry (1-10µg/ml) Immunohistochemistry Immunoprecipitation (1-10µg per 10⁶ pervanadate-treated A431 or HepG2 cells) Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Included (Prod. No. ALX-840-025).

Product Specific Literature References

IL-4-induced priming of human intestinal mast cells for enhanced survival and Th2 cytokine generation is reversible and associated with increased activity of ERK1/2 and c-Fos: A. Lorentz, et al.; J. Immunol. 174, 6751 (2005) Abstract; Full Text

Further Categories Containing This Product:

Phospho-specific Antibodies • Monoclonal Antibodies

ALX-804-288

Revised 20-Sep-07

Monoclonal Antibody to ERK2 (6G11)
SYNONYMS	anti-MAPK2 MAb (6G11) anti-MAP Kinase 2 MAb (6G11)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	ERK1 & ERK2 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-288-C100		100 µg	380.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog
CLONE:	6G11
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:	Synthetic peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2.
SPECIFICITY:	Recognizes the C-terminus of human, mouse, rat and dog ERK2. Does not cross-react with ERK1.
APPLICATION:	Immunoprecipitation Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer milk: 2x PBS, 5-8% (w/v) dry milk powder and 0.1% (v/v) Tween 20.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:	yes

Product Specific Literature References

Further Categories Containing This Product:

Monoclonal Antibodies

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.