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ALX-210-024 Revised 29-Jun-05
Polyclonal Antibody to TRAF2 (human) (Bur 34)
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SYNONYMS anti-Tumor Necrosis Factor (TNF) Receptor-associated Factor 2 (human) PAb (Bur 34)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TRAFs / Related Products
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ALX-210-024-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Antiserum containing PBS and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 6-31(V6TPPGSLELLQPGFSKTLLGTKLEAK31) of human TRAF2 (tumor necrosis factor (TNF) receptor-associated factor 2).
SPECIFICITY: Recognizes human TRAF2. Detects endogenous protein by IHC and WB. Detects a band of ~60-64kDa by Western blot. Does not cross-react with other members of the TRAF family.
APPLICATION: Immunohistochemistry (paraffin sections (1:2'000)
Immunoprecipitation (1:50)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies: J.M. Zapata, et al.; J. Immunol. 165, 5084 (2000) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Polyclonal Antibodies • Autophagy Other Products
 
 
ALX-210-029 Revised 01-Jun-06
Polyclonal Antibody to TRAF2 (mouse) (AR-63)
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SYNONYMS anti-Tumor Necrosis Factor (TNF) Receptor-associated Factor 2 (mouse) PAb (AR-63)
PRODUCT LINE Inflammation
PRODUCT CATEGORY TRAFs / Related Products
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ALX-210-029-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 250-271 (S250FLEAQASPG
TLNQVGPELLQR271) of mouse TRAF2 (tumor necrosis factor (TNF) receptor-associated factor 2).
SPECIFICITY: Recognizes mouse TRAF2. Detects a band of ~60-64kDa by Western blot.
APPLICATION: Immunoprecipitation (1:50)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies: J.M. Zapata, et al.; J. Immunol. 165, 5084 (2000) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Polyclonal Antibodies • Autophagy Other Products
 
 
ALX-210-137/1 Revised 21-Aug-07
Polyclonal Antibody to BiP
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SYNONYMS anti-GRP78 PAb
anti-Glucose Regulated Protein 78 PAb
PRODUCT LINE Stress & Heat Shock Proteins
PRODUCT CATEGORY HSP70 / DnaK / Related Products
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ALX-210-137/1-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
SOURCE/HOST: From rabbit.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Lyophilized. Contains 0.05% sodium azide.
RECONSTITUTION: Reconstitute with PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 643-654 (T643GEEDTSEKDEL654) of rat BiP (GRP78; glucose regulated protein 78kDa).
SPECIFICITY: Recognizes mouse and rat BiP. Does not detect BiP from human cell lines or COS cells.
APPLICATION: Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Endoplasmatic Reticulum Stress • Polyclonal Antibodies • Autophagy Other Products
 
 
ALX-210-206 Revised 25-Sep-08
Polyclonal Antibody to Lysosome-associated Membrane Protein-2
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SYNONYMS anti-LAMP-2 PAb
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Endocytosis
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ALX-210-206-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 400-411 (CG400LKRHHTG
YEQF411) of the cytoplasmic domain of rat LAMP-2 (lysosome-associated membrane protein-2). This sequence is completely conserved in mouse, rat and guinea pig LAMP-2.
SPECIFICITY: Recognizes human, mouse and rat LAMP-2. Detects bands of ~110kDa by Western blot.
APPLICATION: Immunocytochemistry
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-161-004.
Product Specific Literature References
MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking: N. Nakamura, et al.; Mol. Biol. Cell 16, 1696 (2005) Abstract; Full Text
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Further Categories Containing This Product:
Polyclonal Antibodies • Autophagy Other Products
 
 
ALX-260-085 Revised 23-Jan-07
Pepstatin A (synthetic)
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SYNONYMS i-Valeryl-L-Val-L-Val-AHMHA-L-Ala-AHMHA
Iva-Val-Val-Sta-Ala-Sta
PRODUCT LINE Cancer
PRODUCT CATEGORY Cathepsin Inhibitors
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ALX-260-085-M005   5 mg 35.00 USD Add To Cart
ALX-260-085-M025   25 mg 95.00 USD Add To Cart
ALX-260-085-M100   100 mg 290.00 USD Add To Cart
Product Specification
SEQUENCE: i-Valeryl-L-Val-L-Val-AHMHA-L-Ala-AHMHA
(AHMHA = (3S,4S)-4-Amino-3-hydroxy-6-methyl-heptanoic acid)
FORMULA: C34H63N5O9
MW: 685.9
CAS NUMBER: 26305-03-3
MERCK INDEX: 14: 7147
RTECS: SC6155000
SOURCE/HOST: Synthetic.
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, methanol or acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 2 years after receipt when stored at -20°C.
HANDLING: Protect from light.
Product Description
Inhibitor of aspartate (acid) proteases, including pepsin, cathepsin D, renin, chymosin, bacterial aspartic proteinases and HIV proteases. Efficiently inhibits Fcγ receptor induction by IFN-γ. Blocks the endocytic pathway followed by an antibody when it enters the cell at the level of early endosomal compartments, resulting in inhibition of autoantigen presentation or viral transmission.
Product Specific Literature References
Localization of pepstatin's inhibitory action during Fc-mediated antibody internalization: possible implications for antibody-mediated viral transmission: I. Athanassakis, et al.; Cell. Immunol. 199, 81 (2000) Abstract
Pepstatin, a new pepsin inhibitor produced by Actinomycetes: H. Umezawa, et al.; J. Antibiot. 23, 259 (1970) Abstract
Further Categories Containing This Product:
Autophagy Other Products • Proteases Other Products
 
 
ALX-270-078 Revised 16-Jun-06
L-690,330
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SYNONYMS [1-(4-Hydroxyphenoxy)ethylidene]bisphosphonic acid
PRODUCT LINE Inflammation
PRODUCT CATEGORY Lipoxygenases / Related Products
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ALX-270-078-M001   1 mg 30.00 USD Add To Cart
ALX-270-078-M005   5 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C8H12O8P2
MW: 298.1
PURITY: ≥96%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C

Product Description
Potent inositol monophosphatase inhibitor, resistant to hydrolysis.
Product Specific Literature References
In vitro and in vivo inhibition of inositol monophosphatase by the bisphosphonate L-690,330: J.R. Atack, et al.; J. Neurochem. 60, 652 (1993) Abstract
Further Categories Containing This Product:
Autophagy Other Products
 
 
ALX-350-004 Revised 08-Apr-08
Thapsigargin
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Other Tumor Promoters
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ALX-350-004-M001   1 mg 50.00 USD Add To Cart
ALX-350-004-M005   5 mg 200.00 USD Add To Cart
ALX-350-004-M010   10 mg 350.00 USD Add To Cart
ALX-350-004-M025   25 mg 690.00 USD Add To Cart
Product Specification
FORMULA: C34H50O12
MW: 650.8
CAS NUMBER: 67526-95-8
MERCK INDEX: 14: 9272
PURITY: ≥95% (HPLC)
APPEARANCE: Clear colorless film.
SOLUBILITY: Soluble in DMSO or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light and oxygen.
HAZARD: HIGHLY IRRITANT. HARMFUL.

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Product Description
Cell permeable tumor promoter by specific inhibition of the (sarco)endoplasmatic reticulum Ca2+-ATPase (SERCA). Inhibition of SERCA reveals a significant change in intracellular Ca2+ homeostasis and pH regulation in tumor cells. Does not increase inositol phosphates. Shows no effect on protein kinase C (PKC). Increases Ca2+-dependent Na+ influx in human platelets in a dose-dependent manner. Induces apoptosis. Stimulates nitric oxide (NO) production, contributing to hepatocyte apoptosis.
Product Specific Literature References
Brefeldin A, thapsigargin, and AIF4- stimulate the accumulation of GRP78 mRNA in a cycloheximide dependent manner, whilst induction by hypoxia is independent of protein synthesis: B.D. Price, et al.; J. Cell. Physiol. 152, 545 (1992) Abstract
Thapsigargin, a Ca(2+)-ATPase inhibitor, depletes the intracellular Ca2+ pool and induces apoptosis in human hepatoma cells: A. Tsukamoto & Y. Kaneko; Cell Biol. Int. 17, 969 (1993) Abstract
The role of calcium, pH, and cell proliferation in the programmed (apoptotic) death of androgen-independent prostatic cancer cells induced by thapsigargin: Y. Furuya, et al.; Cancer Res. 54, 6167 (1994) Abstract
Intracellular Ca2+ signals activate apoptosis in thymocytes: studies using the Ca(2+)-ATPase inhibitor thapsigargin: S. Jiang, et al.; Exp. Cell Res. 212, 84 (1994) Abstract
Role of EGR-1 in thapsigargin-inducible apoptosis in the melanoma cell line A375-C6: S. Muthukkumar, et al.; Mol. Cell. Biol. 15, 6262 (1995) Abstract; Full Text
Baculovirus p35 and Z-VAD-fmk inhibit thapsigargin-induced apoptosis of breast cancer cells: X.M. Qi, et al.; Oncogene 15, 1207 (1997) Abstract
Signal transduction of thapsigargin-induced apoptosis in osteoblast: H.J. Chae, et al.; Bone 25, 453 (1999) Abstract
Nitric oxide is involved in apoptosis induced by thapsigargin in rat mesangial cells: A.M. Rodriguez-Lopez, et al.; Cell Physiol. Biochem. 9, 285 (1999) Abstract
Thapsigargin induces apoptosis in cultured human aortic smooth muscle cells: C. Peiro, et al.; J. Cardiovasc. Pharmacol. 36, 676 (2000) Abstract
Thapsigargin induces a calmodulin/calcineurin-dependent apoptotic cascade responsible for the death of prostatic cancer cells: B. Tombal, et al.; Prostate 43, 303 (2000) Abstract
Changes in intracellular Ca2+ and pH in response to thapsigargin in human glioblastoma cells and normal astrocytes: G.G. Kovacs, et al.; Am. J. Physiol. Cell. Physiol. 289, C361 (2005) Abstract
Thapsigargin, a selective inhibitor of sarco-endoplasmic reticulum Ca2+ -ATPases, modulates nitric oxide production and cell death of primary rat hepatocytes in culture: N.K. Canova, et al.; Cell Biol. Toxicol. 23, 337 (2007) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Dissolving of 0.65mg Thapsigargin in 1ml gives a stock solution of 1mM.
General Literature References
Role of Ca2(+)-ATPases in regulation of cellular Ca2+ signalling, as studied with the selective microsomal Ca2(+)-ATPase inhibitor, thapsigargin: O. Thastrup; Agents Actions 29, 8 (1990), (Review) Abstract
Thapsigargin, a high affinity and global inhibitor of intracellular Ca2+ transport ATPases: G. Inesi and Y. Sagara; Arch. Biochem. Biophys. 298, 313 (1992), (Review) Abstract
Use of thapsigargin to study Ca2+ homeostasis in cardiac cells: T.B. Rogers, et al.; Biosci. Rep. 15, 341 (1995), (Review) Abstract
The sarcoplasmic reticulum Ca2+ pump: inhibition by thapsigargin and enhancement by adenovirus-mediated gene transfer: G. Inesi, et al.; Ann. NY Acad. Sci. 853, 195 (1998), (Review) Abstract; Full Text
A tool coming of age: thapsigargin as an inhibitor of sarco-endoplasmic reticulum Ca(2+)-ATPases: M. Treiman, et al.; TIPS 19, 131 (1998), (Review) Abstract
Further Categories Containing This Product:
Ca2+-ATPase / Related Products • Endoplasmatic Reticulum Stress • Carcinogens & Tumor Promoters Other Products • Other Natural Products - DNA Regulation / Transcription • Nuclear Envelope & Nuclear Transport • Autophagy Other Products • Natural Products - ATPase Inhibitors • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Nitric Oxide Pathway Modulators
 
 
ALX-350-019 Revised 16-Oct-08
(+)-Brefeldin A
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SYNONYMS BFA
Ascotoxin
Decumbin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Apoptosis Inducers & Inhibitors
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ALX-350-019-M005   5 mg 80.00 USD Add To Cart
ALX-350-019-M010   10 mg 130.00 USD Add To Cart
ALX-350-019-M025   25 mg 270.00 USD Add To Cart
ALX-350-019-M050   50 mg 420.00 USD Add To Cart
Product Specification
FORMULA: C16H24O4
MW: 280.4
CAS NUMBER: 20350-15-6
MERCK INDEX: 14: 1369
SOURCE/HOST: Isolated from Eupenicillium brefeldianum.
PURITY: ≥97% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, methanol, acetone or ethyl acetate.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: HARMFUL.

Product Description
Inhibitor of intracellular protein transport and protein secretion, interfering with trafficking in the trans-Golgi network, leading to the accumulation of cycling proteins in ERGIC clusters. Blocks ADP-ribosylation factor (Arf) in an inactive GDP-bound conformation and thereby prevents binding of COPI coats to ERGIC and Golgi membranes. Upon brefeldin A treatment the Golgi rapidly tubulates and fuses with the ER by an energy-, temperature-, and microtubule-dependent process. In contrast, the drug has little effect on the ERGIC, which keeps its identity, although the ERGIC clusters become larger and more uniformly distributed in the cytoplasm of the cells. Inhibits intracellular collagen degradation. Also inhibits apical Na+ channels in epithelia. Induces apoptosis.
Product Specific Literature References
Decumbin, a new compound from a species of Penicillium: V.L. Singleton, et al.; Nature 181, 1072 (1958)
Über die Isolierung neuer Stoffwechselprodukte aus Penicillium brefeldianum Dodge.: E. Harri, et al.; Helv. Chim. Acta 46, 1235 (1963)
Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes: Y. Misumi, et al.; J. Biol. Chem. 261, 11398 (1986) Abstract; Full Text
Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum: T. Fujiwara, et al.; J. Biol. Chem. 263, 18545 (1988) Abstract; Full Text
Brefeldin A, thapsigargin, and AIF4- stimulate the accumulation of GRP78 mRNA in a cycloheximide dependent manner, whilst induction by hypoxia is independent of protein synthesis: B.D. Price, et al.; J. Cell. Physiol. 152, 545 (1992) Abstract
Brefeldin A and the endocytic pathway. Possible implications for membrane traffic and sorting: W. Hunziker, et al.; FEBS Lett. 307, 93 (1992), (Review) Abstract
Brefeldin A inhibits degradation as well as production and secretion of collagen in human lung fibroblasts: C.R. Ripley, et al.; J. Biol. Chem. 268, 3677 (1993) Abstract
Brefeldin A is a potent inducer of apoptosis in human cancer cells independently of p53: R.G. Shao, et al.; Exp. Cell Res. 227, 190 (1996) Abstract
Brefeldin A inhibition of apical Na+ channels in epithelia: R.S. Fisher, et al.; Am. J. Physiol. 270, C138 (1996) Abstract
Brefeldin A: deciphering an enigmatic inhibitor of secretion: A. Nebenfuhr, et al.; Plant Physiol. 130, 1102 (2002), Review Abstract
NKT cells provide help for dendritic cell-dependent priming of MHC class I-restricted CD8+ T cells in vivo: D. Stober, et al.; J. Immunol. 170, 2540 (2003) Abstract; Full Text
Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70: K.F. Xu, et al.; PNAS 102, 2784 (2005) Abstract
Further Categories Containing This Product:
Golgi Apparatus • Collagen / Related Products • Antitumor Agents (Apoptosis Inducers) • Autophagy Other Products • Na+ Channels • Antibiotics - Antiviral / Anti-HIV • ADP Ribosylation Factor [ARF] / Related Products • Antitumor Antibiotics • Antibiotics - Antifungal
 
 
ALX-350-020 Revised 27-Aug-08
Wortmannin
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SYNONYMS KY 12420
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Protein Kinase Inhibitors
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ALX-350-020-M001   1 mg 40.00 USD Add To Cart
ALX-350-020-M005   5 mg 140.00 USD Add To Cart
ALX-350-020-M025   25 mg 290.00 USD Add To Cart
Product Specification
FORMULA: C23H24O8
MW: 428.4
CAS NUMBER: 19545-26-7