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Matrix Proteins / Related Products
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ALX-210-308 Revised 17-Jul-07
Polyclonal Antibody to Osteocalcin (human) (NT)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Osteocalcin & Matrix Gla-protein [MGP] Family / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-308-R020   20 µl 115.00 USD Add To Cart
ALX-210-308-R100   100 µl 486.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 20µl/100µl distilled water.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-22 (Y1LYQWLGAPVPYP
DPLEPRREV22) of human mature osteocalcin.
SPECIFICITY: Recognizes human osteocalcin. Does not cross-react with human osteopontin, human osteonectin, human bone sialoprotein or bovine or sheep osteocalcin.
APPLICATION: ELISA (1:5'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-309 Revised 26-Mar-08
Polyclonal Antibody to Osteopontin (human)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-309-R020   20 µl 150.00 USD Add To Cart
ALX-210-309-R100   100 µl 660.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 20µl or 100µl distilled water.
IMMUNOGEN: Synthetic peptide corresponding to aa 59-74 (P59SKSNESHDHMDDMDD74) of mature human osteopontin.
SPECIFICITY: Recognizes human osteopontin. Does not cross-react with human osteonectin or bone sialoprotein.
APPLICATION: ELISA (1:1’000)
Immunohistochemistry (paraffin sections (1:1’500))
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-311 Revised 30-May-07
Polyclonal Antibody to Bone Sialoprotein (human)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-311-C010   10 µg 130.00 USD Add To Cart
ALX-210-311-C100   100 µg 577.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From chicken.
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized. Contains PBS, pH 7.4.
RECONSTITUTION: Reconstitute with distilled water.
IMMUNOGEN: Purified human bone sialoprotein (bone extract).
SPECIFICITY: Recognizes human bone sialoprotein. Does not cross-react with human osteopontin, osteonectin or bone alkaline phosphatase.
APPLICATION: ELISA (1µg/ml)
Flow Cytometry
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (2ng/ml))
Western Blot (2ng/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay: M. Karmatschek, et al.; Clin. Chem. 43, 2076 (1997) Abstract; Full Text
Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients: A. Bellahcene, et al.; Br. J. Haematol. 111, 1118 (2000) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-312 Revised 26-Mar-08
Polyclonal Antibody to Bone Sialoprotein
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-312-R020   20 µl 135.00 USD Add To Cart
ALX-210-312-R100   100 µl 660.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 20μl or 100μl distilled water.
IMMUNOGEN: Purified human bone sialoprotein (bone extract).
SPECIFICITY: Recognizes human and rat bone sialoprotein. Does not cross-react with human osteopontin or osteonectin.
APPLICATION: ELISA
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Western Blot
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-313 Revised 14-Nov-06
Polyclonal Antibody to Bone Sialoprotein (human)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-313-R020   20 µl 135.00 USD Add To Cart
ALX-210-313-R100   100 µl 660.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute with 20µl/100µl distilled water.
IMMUNOGEN: Synthetic peptide corresponding to aa 262-279 (Y262ESENGEPRGDNYRAYED279) of human bone sialoprotein.
SPECIFICITY: Recognizes human bone sialoprotein. Does not cross-react with human osteonectin or osteopontin.
APPLICATION: ELISA (1:1'000)
Immunocytochemistry (1:100)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-333 Revised 24-Jul-08
Polyclonal Antibody to Osteocalcin (NT) (mouse)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Osteocalcin & Matrix Gla-protein [MGP] Family / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-333-C100   100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 2mg/ml after reconstitution.
PURITY DETAIL: Protein A-affinity and epitope-affinity purified.
FORMULATION: Lyophilized from 10mM PBS, pH 7.4,  containing 1% BSA and 0.1% sodium azide.
RECONSTITUTION: Reconstitute with 50µl distilled water. Dilute with 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-20 (Y1LGASVPSPDP
LEPTREQCE20) of intracellular N-terminal mouse osteocalcin.
SPECIFICITY: Recognizes intracellular N-terminal mouse osteocalcin. Does not cross-react with rat osteocalcin.
APPLICATION: Immunohistochemistry (paraffin sections (1-2µg/ml))
Note: Not suitable for Western blot.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted solution can be stored at +4°C for 6 months and at -20°C for 1 year.
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-350-019 Revised 16-Oct-08
(+)-Brefeldin A
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SYNONYMS BFA
Ascotoxin
Decumbin
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Apoptosis Inducers & Inhibitors
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ALX-350-019-M005   5 mg 80.00 USD Add To Cart
ALX-350-019-M010   10 mg 130.00 USD Add To Cart
ALX-350-019-M025   25 mg 270.00 USD Add To Cart
ALX-350-019-M050   50 mg 420.00 USD Add To Cart
Product Specification
FORMULA: C16H24O4
MW: 280.4
CAS NUMBER: 20350-15-6
MERCK INDEX: 14: 1369
SOURCE/HOST: Isolated from Eupenicillium brefeldianum.
PURITY: ≥97% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, methanol, acetone or ethyl acetate.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: HARMFUL.

Product Description
Inhibitor of intracellular protein transport and protein secretion, interfering with trafficking in the trans-Golgi network, leading to the accumulation of cycling proteins in ERGIC clusters. Blocks ADP-ribosylation factor (Arf) in an inactive GDP-bound conformation and thereby prevents binding of COPI coats to ERGIC and Golgi membranes. Upon brefeldin A treatment the Golgi rapidly tubulates and fuses with the ER by an energy-, temperature-, and microtubule-dependent process. In contrast, the drug has little effect on the ERGIC, which keeps its identity, although the ERGIC clusters become larger and more uniformly distributed in the cytoplasm of the cells. Inhibits intracellular collagen degradation. Also inhibits apical Na+ channels in epithelia. Induces apoptosis.
Product Specific Literature References
Decumbin, a new compound from a species of Penicillium: V.L. Singleton, et al.; Nature 181, 1072 (1958)
Über die Isolierung neuer Stoffwechselprodukte aus Penicillium brefeldianum Dodge.: E. Harri, et al.; Helv. Chim. Acta 46, 1235 (1963)
Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes: Y. Misumi, et al.; J. Biol. Chem. 261, 11398 (1986) Abstract; Full Text
Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum: T. Fujiwara, et al.; J. Biol. Chem. 263, 18545 (1988) Abstract; Full Text
Brefeldin A, thapsigargin, and AIF4- stimulate the accumulation of GRP78 mRNA in a cycloheximide dependent manner, whilst induction by hypoxia is independent of protein synthesis: B.D. Price, et al.; J. Cell. Physiol. 152, 545 (1992) Abstract
Brefeldin A and the endocytic pathway. Possible implications for membrane traffic and sorting: W. Hunziker, et al.; FEBS Lett. 307, 93 (1992), (Review) Abstract
Brefeldin A inhibits degradation as well as production and secretion of collagen in human lung fibroblasts: C.R. Ripley, et al.; J. Biol. Chem. 268, 3677 (1993) Abstract
Brefeldin A is a potent inducer of apoptosis in human cancer cells independently of p53: R.G. Shao, et al.; Exp. Cell Res. 227, 190 (1996) Abstract
Brefeldin A inhibition of apical Na+ channels in epithelia: R.S. Fisher, et al.; Am. J. Physiol. 270, C138 (1996) Abstract
Brefeldin A: deciphering an enigmatic inhibitor of secretion: A. Nebenfuhr, et al.; Plant Physiol. 130, 1102 (2002), Review Abstract
NKT cells provide help for dendritic cell-dependent priming of MHC class I-restricted CD8+ T cells in vivo: D. Stober, et al.; J. Immunol. 170, 2540 (2003) Abstract; Full Text
Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70: K.F. Xu, et al.; PNAS 102, 2784 (2005) Abstract
Further Categories Containing This Product:
ADP Ribosylation Factor [ARF] / Related Products • Golgi Apparatus • Collagen / Related Products • Na+ Channels • Antitumor Antibiotics • Antitumor Agents (Apoptosis Inducers) • Antibiotics - Antiviral / Anti-HIV • Antibiotics - Antifungal • Autophagy Other Products
 
 
ALX-803-309 Revised 17-Mar-08
Monoclonal Antibody to Collagen Type XII (378D5)
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Collagen / Related Products
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ALX-803-309-R100   100 µl 310.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: 378D5
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites.
IMMUNOGEN: Collagen type XII purified from bovine kidney.
SPECIFICITY: Recognizes human, mouse, rat, bovine and chicken collagen type XII.
APPLICATION: ELISA (1:500-1:1’000)
Immunohistochemistry (1:500-1:1’000): A mild trypsin- or Protease 27 (Sigma)-digestion (0.05% for 10-15min at 37°C) or alternatively microwave oven treatment is recommended to unmask the collagens. Not recommended for paraffin sections.
Immunoprecipitation (1:50-1:100)
Western Blot (1:100-1:300)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-317 Revised 14-Mar-06
Monoclonal Antibody to Osteonectin (AON531)
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PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Matrix Proteins Other Products
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ALX-804-317-C100   100 µg 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: AON531
ISOTYPE: Mouse IgG1
CONCENTRATION:  
FORMULATION: Liquid. In water containing 50% glycerol.
IMMUNOGEN: Human osteonectin.
SPECIFICITY: Recognizes human and bovine osteonectin and human platelet osteonectin.
APPLICATION: ELISA
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-465 Revised 29-Feb-08
Monoclonal Antibody to CCL2 (human) (5D3-F7)
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SYNONYMS anti-MCP-1 (human) MAb (5D3-F7)
anti-Monocyte Chemotactic Protein-1 (human) MAb (5D3-F7)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CC Chemokines & Receptors / Related Products
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ALX-804-465-C100   100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 5D3-F7
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Purified antibody in PBS containing 0.1% sodium azide.
IMMUNOGEN: Recombinant human CCL2 (monocyte chemotactic protein-1; MCP-1).
SPECIFICITY: Recognizes human CCL2.
APPLICATION: ELISA (capture)
Immunocytochemistry (1:1'000)
Immunohistochemistry (frozen sections, paraffin sections (1:250-1:500))
Immunoprecipitation
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
A new monoclonal antibody (5D3-F7) which recognizes human monocyte chemotactic protein-1 but not related chemokines. Development of a sandwich ELISA and in situ detection of producing cells: G. Peri, et al.; J. Immunol. Methods 174, 249 (1994) Abstract
Analysis of the Gene Expression Profile Activated by the CC Chemokine Ligand 5/RANTES and by Lipopolysaccharide in Human Monocytes: M. Locati, et al.; J. Immunol. 168, 3557 (2002) Full Text
Human Pancreatic Islets Produce and Secrete MCP-1/CCL2: Relevance in Human Islet Transplantation: L. Piemonti, et al.; Diabetes 51, 55 (2002) Full Text
Further Categories Containing This Product:
Osteocalcin & Matrix Gla-protein [MGP] Family / Related Products • Monoclonal Antibodies
 
 
ALX-804-512 Revised 22-Apr-08
Monoclonal Antibody to Matrix Gla Protein (52.1C5D)
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SYNONYMS anti-MGP MAb (52.1C5D)
PRODUCT LINE Bone Metabolism
PRODUCT CATEGORY Osteocalcin & Matrix Gla-protein [MGP] Family / Related Products
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ALX-804-512-C100   100 µg 490.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Pig
Others
CLONE: 52.1C5D
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Lyophilized from PBS containing 0.05% sodium azide.
RECONSTITUTION: Reconstitute with 100µl distilled water.
IMMUNOGEN: Synthetic peptide corresponding to aa 3-15 of non-phosphorylated human MGP (matrix Gla protein).
SPECIFICITY: Recognizes human, rat (60%), mouse (29%), pig (15%) and horse (19%) carboxylated (functional) and non-carboxylated (non-functional) MGP.
APPLICATION: ELISA (1:1’000)
Immunohistochemistry (frozen sections (1:200), paraffin sections (1:200))
Western Blot (1:500)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 month when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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