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Monoclonal Antibodies
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ALX-804-133 Revised 27-Mar-07
Human IgG1 Isotype Control
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PRODUCT LINE Immunology
PRODUCT CATEGORY Isotype Controls / Immunoglobulins
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-133-C100   100 µg 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
ISOTYPE: Human IgG1κ
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.1EU/µg antibody (LAL test; BioWhittaker)
RECONSTITUTION: Reconstitute with 100µl sterile water.
APPLICATION: Negative control in assays where receptors:Fc (IgG1) are used:
ELISA
Flow Cytometry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
Product Description
Human IgG1 are purified from human plasma by fractionation, ion exchange and affinity chromatography. The purified IgG represent a single subclass and light chain type (κ). The subclass was determined by subclass monoclonal antibodies and type specific polyclonal antibodies in immunoelectrophoresis, Ouchterlony and indirect ELISA assays.
Source material has been tested and found NEGATIVE for antibodies to HIV and HbsAgs.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-590 Revised 09-Jan-09
M30 CytoDEATH™ Monoclonal Antibody
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytokeratin-based Apoptosis Detection
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-590-T200   200 tests 465.00 USD Add To Cart
ALX-804-590B-T200 Biotin 200 tests 495.00 USD Add To Cart
ALX-804-590F-T200 FITC 200 tests 495.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
Others
CLONE: M30
ISOTYPE: Mouse IgG2b
CONCENTRATION: M30 & M30-FITC: 0.05mg/ml
M30-Biotin: 0.25mg/ml
FORMULATION: Liquid. In 200μl PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
IMMUNOGEN: CK18 (cytokeratin 18) fragments purified from supernatant from human carcinoma cell line WiDr CCL218.
SPECIFICITY: Recognizes the CK18-Asp396 neo-epitope (M30) on human, monkey and bovine caspase-cleaved CK18. Does not cross-react with intact CK18.
APPLICATION: M30 & M30-Biotin:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections; paraffin sections)
Western Blot

M30-FITC:
Flow Cytometry
Immunocytochemistry

A dilution of 1:100 in incubation buffer is generally recommended. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Specific Literature References
Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis: M.P. Leers, et al.; J. Pathol. 187, 567 (1999) Abstract
Apoptosis of circulating tumor cells in prostate cancer patients: C. J. Larson, et al.; Cytometry A. 62, 46 (2004) Abstract
Uncovering the roles of intermediate filaments in apoptosis: N. Marceau; Methods Cell Biol. 78, 95 (2004) Abstract
Maternal smoking, intrauterine growth restriction, and placental apoptosis: C. Vogt Isaksen; Pediatr. Dev. Pathol. 7, 433 (2004) Abstract
Modular effects of estradiol on ethanol-induced apoptosis in human intestinal epithelial cells: K. Asai, et al.; Scand. J. Gastroenterol. 40, 326 (2005) Abstract
Apoptosis resistance in Barrett’s esophagus: ex vivo bioassay of live stressed tissues: K. Dvorakova, et al.; Am. J. Gastroenterol. 100, 424 (2005) Abstract
Oncosis represents the main type of cell death in mouse models of cholestasis: P. Fickert, et al.; J. Hepatol. 42, 378 (2005) Abstract
Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome: a differential expression study: P.Y. Fong, et al.; Cancer 103, 268 (2005) Abstract
Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo-stressed colonic mucosa: H. Holubec, et al.; J. Histochem. Cytochem. 53, 229 (2005) Abstract
Inhibition of bcl-2 enhances the efficacy of chemotherapy in renal cell carcinoma: I. Kausch, et al.; Eur. Urol. 47, 703 (2005) Abstract
Expression of tumour necrosis factor-related apoptosis-inducing ligand death receptors in sporadic and hereditary colorectal tumours: Potential targets for apoptosis induction: J.J. Koornstra, et al.; Eur. J. Cancer 41, 1195 (2005) Abstract
Expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in human preeclamptic placenta: possible implications in the process of trophoblast apoptosis: H. Lee, et al.; Placenta 26, 226 (2005) Abstract
Role of bax mutations in apoptosis in colorectal cancers with microsatellite instability: C. Miquel, et al.; Am. J. Clin. Pathol. 123, 562 (2005) Abstract
Fas ligand expression by maternal decidual cells is negatively correlated with the abundance of leukocytes present at the maternal-fetal interface: Q. Qiu, et al.; J. Reprod. Immunol. 65, 121 (2005) Abstract
Differential susceptibility of metastatic and primary oral cancer cells to TRAIL-induced apoptosis: N. Vigneswaran, et al.; Int. J. Oncol. 26, 103 (2005) Abstract
Effects of vitamin restriction and supplementation on rat intestinal epithelial cell apoptosis: B. Vijayalakshmi, et al.; Free Radic. Biol. Med. 38, 1614 (2005) Abstract
Expression levels of Akt in nimesulide-treated squamous carcinoma cell lines of the head and neck: L. Vormittag, et al.; Oncol. Rep. 13, 207 (2005) Abstract
For a comprehensive bibliography please click here:
General Information
BACKGROUND/TECHNICAL INFORMATION Cytokeratin 18 expression has been shown in but is not limited to cells and tissues from lung, breast, intestine, gut, colon, kidney, pancreas, bladder, trophoblasts ... etc.
The M30 CytoDEATH™ monoclonal antibody has been shown in numerous independent studies to be specific to the CK18-Asp396 caspase cleavage site (neo-epitope (M30)) and represents a well-established, validated apoptosis marker that does not react with viable or necrotic cells.
M30 CytoDEATH™ detects human, monkey and bovine apoptotic epithelial cells and tissues and has been validated for many applications including immunohistochemistry of formalin-fixed paraffin-embedded (FFPE) tissues.
Apoptosis detection using M30 CytoDEATH™ has been described in several publications to serve as a superior technique compared to TUNEL or detection of γH2AX phosphorylation. Assessment of apoptosis by immunohistochemical markers compared to cellular morphology in ex vivo‑stressed colonic mucosa under conditions of DNA-damage may be repaired and results in false-positive counts of apoptotic cells. In addition has modest or transient caspase activation without the cleavage of death substrates been attributed to non-suicidal processes such as proliferation, differentiation and inflammation.

For more information on procedures and materials required please see also the Original Manufacturers Product Description
(click here to download pdf for M30 CytoDEATH™ Monoclonal Antibody)
(click here to download pdf for M30 CytoDEATH™ Monoclonal Antibody (Biotin))
(click here to download pdf for M30 CytoDEATH™ Monoclonal Antibody (FITC))
MANUFACTURER Manufactured by Peviva AB.
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • Tumor Markers
 
 
ALX-801-077 Revised 15-Jan-08
Monoclonal Antibody to 90K/Mac-2 BP (human) (SP-2)
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PRODUCT LINE Cancer
PRODUCT CATEGORY Tumor Markers
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-077-C100   100 µg 259.00 USD Add To Cart
ALX-801-077F-T100 FITC 100 tests 308.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: SP-2
ISOTYPE: Mouse IgG1
PURITY: ≥95% (SDS-PAGE)
FORMULATION: SP-2: Liquid. 0.1mg/ml in PBS.
SP-2-FITC: Liquid. 100 tests in 1ml 50mM TRIS, pH 7.4, containing 100mM NaCl, 1% BSA and 0.02% sodium azide.
IMMUNOGEN: Human 90K/Mac-2 BP.
SPECIFICITY: Recognizes the Mac-2 mucin-like binding domain of human 90K/Mac-2 BP.
APPLICATION: Immunohistochemistry (acetone fixed frozen sections, paraffin sections, cell smears (5µg/ml))
Western Blot (non-denaturating conditions)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Specific Literature References
Purification and characterization of a 90 kDa protein released from human tumors and tumor cell lines: S. Iacobelli, et al.; FEBS Lett. 319, 59 (1993) Abstract
The secreted tumor-associated antigen 90K is a potent immune stimulator: A. Ullrich, et al.; J. Biol. Chem. 269, 18401 (1994) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-137 Revised 07-Dec-05
Monoclonal Antibody to A20 (human) (8E8.38)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY TLRs Other Products
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ALX-804-137-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 8E8.38
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Zinc finger domain corresponding to aa 375-537 of human A20.
SPECIFICITY: Recognizes human A20.
APPLICATION: Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
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Product Description
A20 is a zinc finger protein that renders cells resistant to apoptosis. A20-deficient mice develop severe inflammation and are hyper-responsive to LPS. S.M. O'Reilly & P.N. Moynagh have shown that full length A20 inhibits activation of NF-kB and AP-1 by TLR4 [1].  A20 associates with A20-binding inhibitor of NF-kB (ABIN)-1 and -2 [2-4], which interfere with NF-kB activation upstream of the IKK complex.
Product Specific Literature References
A20 inhibits NF-kappa B activation downstream of multiple Map3 kinases and interacts with the I kappa B signalosome: F.S. Zetoune, et al.; Cytokine 15, 282 (2001) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Regulation of Toll-like receptor 4 signalling by A20 zinc finger protein: S.M. O'Reilly and P.N. Moynagh; Biochem. Biophys. Res. Commun. 3303, 586 (2003) Abstract
[2] The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN: K. Heyninck, et al.; J. Cell Biol. 145, 1471 (1999) Abstract; Full Text
[3] Identification of a novel A20-binding inhibitor of nuclear factor-kappa B activation termed ABIN-2: S. Van Huffel, et al.; J Biol Chem 276, 30216 (2001) Abstract; Full Text
[4] Structure-function analysis of the A20-binding inhibitor of NF-kappa B activation, ABIN-1: K. Heyninck, et al.; FEBS Lett. 536, 135 (2003) Abstract
Further Categories Containing This Product:
NF-kB Pathway Other Products • Monoclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products
 
 
ALX-804-722 Revised 03-Dec-08
Monoclonal Antibody to ACE2 (human) (AC384)
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SYNONYMS anti-Angiotensin-converting Enzyme 2 (human) MAb (AC384)
anti-SARS Receptor (human) MAb  (AC384)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-722-C050   50 µg 195.00 USD Add To Cart
ALX-804-722-C100   100 µg 270.00 USD Add To Cart
ALX-804-722B-C050 Biotin 50 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: AC384
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human ACE2 (angiotensin-converting enzyme 2).
SPECIFICITY: Recognizes human ACE2.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:10’000)
Western Blot (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for up to 6 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • Monoclonal Antibodies
 
 
ALX-804-715 Revised 19-Jun-08
Monoclonal Antibody to ACE2 (human) (AC18F)
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SYNONYMS anti-Angiotensin-converting Enzyme 2 (human) MAb (AC18F)
anti-SARS Receptor (human) MAb (AC18F)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Angiotensin Converting Enzyme [ACE] & ACE2 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-715-C050   50 µg 195.00 USD Add To Cart
ALX-804-715-C100   100 µg 270.00 USD Add To Cart
ALX-804-715B-C050 Biotin 50 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: AC18F
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human ACE2 (angiotensin-converting enzyme 2).
SPECIFICITY: Recognizes human ACE2.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:10’000)
Flow Cytometry
Western Blot (1:2’000-1:10’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Specific Literature References
Co-localization of angiotensin-converting enzyme 2-, octomer-4- and CD34 positive cells in rabbit atherosclerotic plaques: A. Zulli, et al.; Exp. Physiol. 93, 564 (2008) Abstract
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
SARS Coronavirus / Related Products • Monoclonal Antibodies
 
 
ALX-802-004 Revised 18-Feb-08
Monoclonal Antibody to Acetylcholinesterase (ZR3)
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SYNONYMS anti-AChE MAb (ZR3)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Acetylcholine Esterase / Related Products
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ALX-802-004-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Rat
Others
CLONE: ZR3
ISOTYPE: Mouse IgG2b
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Purified rat brain AChE (acetylcholinesterase).
SPECIFICITY: Recognizes rat, guinea pig, rabbit and cat AChE. Does not cross-react with BChE (butyrylcholinesterase).
APPLICATION: Immunohistochemistry (1:1'000)
Immunoprecipitation
Not recommended for Western blot.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies to rat brain acetylcholinesterase: comparative affinity for soluble and membrane-associated enzyme and for enzyme from different vertebrate species: Z. Rakonczay and S. Brimijoin; Neurochemistry 46, 280 (1986) Abstract
Selective destruction of preganglionic sympathetic nerves by antibodies to acetylcholinesterase: S. Brimijoin and V.A. Lennon; J. Neural Transm. Suppl. 34, 139 (1991) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION

Reacts with both the soluble and membrane-associated AChE. Immunohistochemical staining of AChE in rat brain results in staining of nerve fibers and terminals.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-802-003 Revised 10-Dec-07
Monoclonal Antibody to Acetylcholinesterase (HR2)
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SYNONYMS anti-AChE MAb (HR2)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Acetylcholine Esterase / Related Products
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ALX-802-003-R200   200 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: HR2
ISOTYPE: Mouse IgG2b
FORMULATION: Liquid. Diluted ascites in PBS containing 0.05% sodium azide.
IMMUNOGEN: Purified human brain AChE (acetylcholinesterase).
SPECIFICITY: Recognizes human, rabbit, guinea pig, bovine and cat AChE. Does not cross-react with BChE (butyrylcholinesterase).
APPLICATION: ELISA
Immunohistochemistry (frozen sections (1:50))
Immunoprecipitation
Note: Not suitable for Western blot.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibodies to human brain acetylcholinesterase: properties and applications: Z. Rakonczay and S. Brimijoin; Cell. Mol. Neurobiol. 8, 85 (1988)
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-391 Revised 02-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (10D8)
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SYNONYMS anti-GlcNAc MAb (10D8)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
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ALX-804-391-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 10D8
ISOTYPE: Mouse IgM
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: N-Acetylglucosamine.
SPECIFICITY: Recognizes human, mouse and rat N-acetylglucosamine.
APPLICATION: Western Blot (1 µg/ml for AP/BCIP/NBT (MTT) detection; 0.5 µg/ml for HRPO/ECL detection).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-276 Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (HGAC85)
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SYNONYMS anti-Glc-NAc (O-linked) MAb (HGAC85)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
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ALX-804-276-C100   100 µg 379.00 USD Add To Cart
Product Specification
CLONE: HGAC85
ISOTYPE: Mouse IgG3
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Heat killed, pepsin treated group A streptococci.
SPECIFICITY: Recognizes β-1,3 linked O-GlcNAc (O-linked N-acetylglucosamine) residues of streptococcal group A carbohydrates as well as O-GlcNAc glycosylated proteins.
APPLICATION: ELISA
Immunocytochemistry (10µg/ml)
Immunoprecipitation
Western Blot (1µg/ml)
Note: Do not use with diluents containing glycosylated proteins!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Identification of O-linked N-acetylglucosamine modification of ankyrinG isoforms targeted to nodes of Ranvier: X. Zhang & V. Bennett; J. Biol. Chem. 271, 31391 (1996) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-111 Revised 15-Oct-07
Monoclonal Antibody to N-Acetylglucosamine (O-linked) (RL2)
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SYNONYMS anti-Glc-NAc (O-linked) MAb (RL2)
PRODUCT LINE Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY Protein Glycosylation
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ALX-804-111-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: RL2
ISOTYPE: Mouse IgG1
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Pore complex-lamina fraction purified from rat liver nuclear envelopes.