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CD Antibodies
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ALX-804-153 Revised 01-Sep-05
Monoclonal Antibody to TACI (human) (1A1)
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SYNONYMS anti-Transmembrane Activator and CAML Interactor (human) MAb (1A1)
anti-TNFRSF 13B (human) MAb (1A1)
anti-CD267 (human) MAb (1A1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TACI
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-153-C050   50 µg 230.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 1A1
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Rat RBL cells expressing human TACI.
SPECIFICITY: Recognizes human TACI.
APPLICATION: Flow Cytometry
Note: Not recommended for Immunohistochemistry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
B Cell-Activating Factor Belonging to the TNF Family (BAFF)-R Is the Principal BAFF Receptor Facilitating BAFF Costimulation of Circulating T and B Cells: L.G. Ng, et al.; J. Immunol. 173, 807 (2004) Abstract
Mutations in TNFRSF13B encoding TACI are associated with common variable immunodeficiency in humans: U. Salzer, et al.; Nat. Genet. 37, 820 (2005) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-157 Revised 12-Jun-08
Monoclonal Antibody to BAFF (mouse) (1C9)
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SYNONYMS anti-BlyS (mouse) MAb (1C9)
anti-TALL-1 (mouse) MAb (1C9)
anti-THANK (mouse) MAb (1C9)
anti-zTNF4 (mouse) MAb (1C9)
anti-TNFSF 13B/20 (mouse) MAb (1C9)
anti-CD257 (mouse) MAb (1C9)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-157-C100   100 µg 360.00 USD Add To Cart
ALX-804-157B-C100 Biotin 100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 1C9
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE).
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Rat RBL cells expressing mouse BAFF.
SPECIFICITY: Recognizes mouse BAFF.
APPLICATION: ELISA (detection)
Flow Cytometry
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-158 Revised 25-May-05
Monoclonal Antibody to BAFF (mouse) (5A8)
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SYNONYMS anti-BlyS (mouse) MAb (5A8)
anti-TALL-1 (mouse) MAb (5A8)
anti-THANK (mouse) MAb (5A8)
anti-zTNF4 (mouse) MAb (5A8)
anti-TNFSF 13B/20 (mouse) MAb (5A8)
anti-CD257 (mouse) MAb (5A8)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-158-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 5A8
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
FORMULATION: Liquid. Purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Rat RBL cells expressing mouse BAFF.
SPECIFICITY: Recognizes mouse BAFF.
APPLICATION: ELISA (capture)
Flow Cytometry (excellent)
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-159 Revised 30-Jun-05
Monoclonal Antibody to BAFF (human) (4D3)
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SYNONYMS anti-BLyS (human) MAb (4D3)
anti-TALL-1 (human) MAb (4D3)
anti-THANK (human) MAb (4D3)
anti-zTNF4 (human) MAb (4D3)
anti-TNFSF 13B/20 (human) MAb (4D3)
anti-CD257 (human) MAb (4D3)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-159-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 4D3
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: 95% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Recombinant human BAFF.
SPECIFICITY: Recognizes human BAFF.
APPLICATION: Flow Cytometry
Immunohistochemistry (frozen sections)
Functional Application (inhibition)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-201 Revised 23-Jun-08
Monoclonal Antibody to E-cadherin (HECD-1)
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SYNONYMS anti-Uvomorulin MAb (HECD-1)
anti-CD324 MAb (HECD-1)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Cadherin Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-201-C100   100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: HECD-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7.
CONCENTRATION: 2mg/ml after reconstitution.
PURITY DETAIL: Ammonium sulfate precipitation and ion exchange chromatography.
FORMULATION: Lyophilized from 10mM PBS, pH 7.4 , containing 1% BSA. Contains no preservatives.
RECONSTITUTION: Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN: Human breast tumor cell line MCF-7.
SPECIFICITY: Recognizes human and guinea pig E-cadherin. Does not cross-react with rat, dog, bovine or chicken E-cadherin.
APPLICATION: ELISA (2-10µg/ml)
Flow Cytometry (1µg/ml)
Immunohistochemistry (frozen sections (2-10µg/ml), paraffin sections (2-10µg/ml))
Western Blot (2-10µg/ml)
Functional Application (100µg/ml for inhibition of E-cadherin dependent cell-cell contact)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas: Y. Shimoyama, et al.; Cancer Res. 49, 2128 (1989) Abstract
Expression of immunoreactive E-cadherin adhesion molecules in human cancers: H. Shiozaki, et al.; Am. J. Pathol. 139, 17 (1991) Abstract
Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections: S.R. Shi, et al.; J. Histochem. Cytochem. 39, 741 (1991) Abstract
Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma: Y. Shimoyama & S. Hirohashi; Cancer Lett. 57, 131 (1991) Abstract
Expression of E- and P-cadherin in gastric carcinomas: Y. Shimoyama & S. Hirohashi; Cancer Res. 51, 2185 (1991) Abstract
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991) Abstract
Immunohistochemical localization of cell adhesion molecule epithelial cadherin in human arachnoid villi and meningiomas: Y. Tohma, et al.; Cancer Res. 52, 1981 (1992) Abstract
J.A. Jankowski, et al.; Int. J. Oncol. 4, 441 (1994)
CD123bright plasmacytoid predendritic cells: progenitors undergoing cell fate conversion?: M.R. Comeau, et al.; J. Immunol. 169, 75 (2002) Abstract; Full Text
Changing etiology of tubal pregnancy following IVF: A. Revel, et al.; Hum. Reprod. 6, 1372 (2008) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Ca2+ should be contained in all buffers to stabilize cadherin antigen.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-202 Revised 23-Sep-08
Monoclonal Antibody to E-cadherin (ECCD-2)
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SYNONYMS anti-Uvomorulin MAb (ECCD-2)
anti-CD324 MAb (ECCD-2)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Cadherin Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-202-C100   100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
CLONE: ECCD-2
ISOTYPE: Rat IgG2a
SOURCE/HOST: Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment.
CONCENTRATION: 2mg/ml after reconstitution.
PURITY DETAIL: Purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography.
FORMULATION: Lyophilized from 20mM TBS, pH 7.5, containing 1% BSA. Contains no preservatives.
RECONSTITUTION: Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1.0% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
IMMUNOGEN: Mouse liver E-cadherin fragment.
SPECIFICITY: Recognizes human, mouse and dog E-cadherin. Does not cross-react with bovine or chicken E-cadherin.
APPLICATION: Immunohistochemistry (frozen sections (10µg/ml), not recommended for paraffin sections)
Western Blot (10µg/ml)
Functional Application Does not inhibit E-cadherin dependent cell-cell contact and can be used to analyze the mechanism.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin: Y. Shirayoshi, et al.; Cell Struct. Funct. 11, 245 (1986) Abstract
Transformation of cell adhesion properties by exogenously introduced E- cadherin cDNA: A. Nagafuchi, et al.; Nature 329, 341 (1987) Abstract
The cadherins: cell-cell adhesion molecules controlling animal morphogenesis: M. Takeichi; Development 102, 639 (1988) Abstract
Cadherin cell adhesion receptors as a morphogenetic regulator: M. Takeichi; Science 251, 1451 (1991) Abstract
Recognition of E-cadherin on epithelial cells by the mucosal T cell integrin alpha M290 beta 7 (alpha E beta 7): P.I. Karecla, et al.; Eur. J. Immunol. 25, 852 (1995) Abstract
Role of IQGAP1, a target of the small GTPase Cdc42 and Rac1, in regulation of E-cadherin-mediated cell-cell adhesion: S. Kuroda, et al.; Science 281, 832 (1998) Abstract
The alpha isoform of protein kinase C is involved in signaling the response of desmosomes to wounding in cultured epithelial cells: S. Wallis, et al.; Mol. Biol. Cell. 11, 1077 (2000) Abstract
Recruitment and activation of Rac1 by the formation of E-cadherin- mediated cell-cell adhesion sites: M. Nakagawa, et al.; J. Cell. Sci. 114, 1829 (2001) Abstract
Inverse correlation between the extent of N-glycan branching and intercellular adhesion in epithelia. Contribution of the Na,K-ATPase beta1 subunit: O. Vagin, et al.; J. Biol. Chem. 283, 2192 (2008) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Buffers should contain Ca2+ to stabilize cadherin antigen. In case of immunohistochemical staining of acetone fixed frozen sections, Ca2+ is not necessary.
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Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-203 Revised 06-Mar-08
Monoclonal Antibody to E-cadherin (human) (SHE78-7)
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SYNONYMS anti-Uvomorulin (human) MAb (SHE78-7)
anti-CD324 (human) MAb (SHE78-7)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Cadherin Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-203-C100   100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: SHE78-7
ISOTYPE: Mouse IgG2a
SOURCE/HOST: Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with soluble E-cadherin from human placenta.
CONCENTRATION: 2mg/ml after reconstitution.
PURITY DETAIL: Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography.
FORMULATION: Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA. Contains no preservatives.
RECONSTITUTION: Reconstitute with 50µl distilled water. Dilute with 20mM TBS, pH 7.4, 10mM CaCl2, 1% BSA. Reconstituted solution should contain 0.1% sodium azide as a preservative when stored at +4°C.
Note: TBS should be used for dilution, as PBS causes precipitation.
IMMUNOGEN: Soluble E-cadherin from human placenta.
SPECIFICITY: Recognizes human E-cadherin. Does not cross-react with rat E-cadherin.
APPLICATION: ELISA (2µg/ml)
Flow Cytometry (1µg/ml)
Immunohistochemistry (frozen sections (2µg/ml), paraffin sections (2µg/ml); good results were obtained by incubating with the primary antibody at 4°C overnight);
Western Blot (2µg/ml; reducing and non-reducing conditions);
Functional Application (1µg/ml for inhibition of E-cadherin-dependent cell-cell contact)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Soluble E-cadherin fragments increased in circulation of cancer patients: M. Katayama, et al.; Br. J. Cancer 69, 580 (1994) Abstract
Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line: M. Watabe, et al.; J. Cell. Biol. 127, 247 (1994) Abstract; Full Text
Soluble fragment of E-cadherin cell adhesion molecule increase in urinary excretion of cancer patients, potentially indicating its shedding from epithelial tumour cells: M. Katayama, et al.; Int. J. Oncol. 5, 1049 (1994)
General Information
BACKGROUND/TECHNICAL INFORMATION Ca2+ should be contained in the buffers to stabilize cadherin antigen. In case of immunohistochemical staining of paraffin embedded tissues and acetone fixed frozen sections, Ca2+ is not necessary.
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-212 Revised 17-Sep-07
Monoclonal Antibody to RANK (human) (9A725)
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SYNONYMS anti-TRANCE-R (human) MAb (9A725)
anti-ODFR (human) MAb (9A725)
anti-TNFRSF 11A (human) MAb (9A725)
anti-CD265 (human) MAb (9A725)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-212-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 9A725
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human RANK (aa 326-616).
SPECIFICITY: Recognizes an epitope between aa 330-427 of human RANK. Detects a band of ~97kDa by Western blot.
APPLICATION: Flow Cytometry (intracellular) (see [1])
Immunohistochemistry (paraffin sections)
Western Blot  (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Images
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Product Specific Literature References
[1] Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
[2] A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004) Abstract
[3] Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism: A.C. Bharti, et al.; J. Biol. Chem. 279, 6065 (2004) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-231 Revised 07-May-08
Monoclonal Antibody to FasL (human) (5G51)
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SYNONYMS anti-CD95L (human) MAb (5G51)
anti-APO-1L (human) MAb (5G51)
anti-CD178 (human) MAb (5G51)
anti-TNFSF 6 (human) MAb (5G51)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-231-C100   100 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 5G51
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In 0.15M PBS, pH 7.2 containing 1% BSA. Contains no preservatives.
IMMUNOGEN: Recombinant human FasL (CD95L; APO-1L; CD178) (extracellular domain).
SPECIFICITY: Recognizes human FasL.
APPLICATION: Flow Cytometry (excellent)
Immunohistochemistry (frozen sections, paraffin sections)
Functional Application (neutralizing).
Suggested starting dilution is 1:100. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
HANDLING: Do not freeze/thaw. Keep sterile.
Product Images
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Product Specific Literature References
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt et al.; Eur. J. Immunol. 30, 1769 (2000) Abstract
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