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ALX-201-063
Revised 25-Oct-06
PARP-1 (human) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-063-C020
20 µg
348.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
1mg/ml.
PURITY:
≥99% (SDS-PAGE
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. In 100mM TRIS-HCl, pH 7.5,containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:
≥600U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C, pH 7.5.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Overproduction and large-scale purification of the human poly(ADP- ribose) polymerase using a baculovirus expression system:
H. Giner, et al.; Gene
114
, 279 (1992)
Abstract
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing
:
M. Malanga & F.R. Althaus; J. Biol. Chem.
279
, 5244 (2004)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Related Products
ALX-840-040
DNA (purified) (DNAse I activated)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-254
Revised 18-Mar-08
PARP-1 (E988K mutant) (human) (recombinant) (Control)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (E988K mutant) (human) (recombinant) (Control)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-254-C010
10 µg
180.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length inactive mutant E988K of PARP-1 is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY:
0.5% of wild type PARP-1.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function:
P.O. Hassa, et al.; J. Biol. Chem.
276
, 45588 (2001)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-250
Revised 18-Mar-08
PARP-1 (human) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-250-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length PARP-1 is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY:
≥1’200U/mg protein.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1:
P.O. Hassa, et al.; J. Biol. Chem.
278
, 45145 (2003)
Abstract
;
Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription:
P.O. Hassa, et al.; J. Biol. Chem.
280
, 40450 (2005)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-255
Revised 18-Mar-08
PARP-1 (BRCT Domain) (human) (recombinant) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (BRCT Domain) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-201-255-C020
20 µg
290.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Human PARP-1 (BRCT domain) (aa 385-524) is fused to a His-tag.
CONCENTRATION:
1mg/ml
PURITY:
≥99% (SDS-PAGE)
PURITY DETAIL:
Affinity purified on Ni-beads.
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 8, containing 200mM sodium chloride, 60mM imidazole, 0.5mM PMSF and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Related Products
ALX-210-540
Polyclonal Antibody to PARP-1 (BRCT Domain)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-232
Revised 18-Mar-08
PARP-1 (Catalytic Domain) (human) (recombinant) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (Catalytic Domain) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-232-C020
20 µg
380.00 USD
Product Specification
SOURCE/HOST:
Produced in SF21 cells. Human PARP-1 (aa 656-1014) is fused to a His-tag.
CONCENTRATION:
1.5mg/ml.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-253
Revised 18-Mar-08
PARP-1b (human) (recombinant) (active) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1b (human) (recombinant) (active) (His)
short-form PARP-1 (human) (recombinant) (active) (His)
sPARP-1 (human) (recombinant) (active) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-253-C020
20 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human PARP-1b (short-form PARP1; sPARP-1) is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Characterization of sPARP-1. An alternative product of PARP-1 gene with poly(ADP-ribose) polymerase activity independent of DNA strand breaks:
F.R. Sallmann, et al.; J. Biol. Chem.
275
, 15504 (2000)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
General Literature References
Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going?:
P.O. Hassa, et al.; Microbiol. Mol. Biol. Rev.
70
, 789 (2006)
Abstract
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-064
Revised 25-Oct-06
PARP-2 (mouse) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-2 (mouse) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-2
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-064-C020
20 µg
368.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
~1mg/ml
PURITY:
≥98% (SDS-PAGE)
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. In 100mM TRIS-HCl, pH 7.5, containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:
≥380U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min at 25°C, pH 7.5. See also separate protocol (link below).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase:
J.C. Ame, et al.; J. Biol. Chem.
274
, 17860 (1999)
Abstract
;
Full Text
A bidirectional promoter connects the poly(ADP-ribose) polymerase 2 (PARP-2) gene to the gene for RNase P RNA. structure and expression of the mouse PARP-2 gene:
J.C. Ame, et al.; J. Biol. Chem.
276
, 11092 (2001)
Abstract
;
Full Text
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing
:
M. Malanga & F.R. Althaus; J. Biol. Chem.
279
, 5244 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link O88554:
PARP-2 (mouse)
Related Products
ALX-840-040
DNA (purified) (DNAse I activated)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-251
Revised 18-Mar-08
PARP-2 (mouse) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-2 (mouse) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-2
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-251-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Mouse full-length PARP-2 is fused to a His-tag.
CONCENTRATION:
1mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link O88554:
PARP-2 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-170
Revised 25-Oct-06
PARP-3 (human) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-3 (human) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-3
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-170-C020
20 µg
368.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
0.38mg/ml
PURITY:
≥97%
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 0.25mM EDTA, 7mM β-mercaptoethanol, 0.25mM PMSF and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression
:
A. Augustin, et al.; J. Cell Sci.
116
, 1551 (2003)
Abstract
;
Full Text
Tankyrase-1 polymerization of poly(ADP-ribose) is required for spindle structure and function:
P. Chang et al.; Nature Cell Biology
7
, 1133 (2005)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-252
Revised 18-Mar-08
PARP-3 (human) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-3 (human) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-3
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-252-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length PARP-3 is fused to a His-tag.
CONCENTRATION:
1mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q9Y6F1:
PARP-3 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-150
Revised 05-Jul-06
Peptidyl-prolyl cis-trans Isomerase CPR6 (yeast) (recombinant)
SYNONYMS
CPR6 (yeast) (recombinant)
PPIase Cpr6 (yeast) (recombinant)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Immunophilins / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-150-C025
25 µg
290.00 USD
Product Specification
EC:
5.2.1.8
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
23mg/ml
PURITY:
≥99%
FORMULATION:
Liquid. In 40mM HEPES/KOH, pH 7.5, containing 20mM potassium chloride.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-80°C
HANDLING:
For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
Product Specific Literature References
Cpr6 and Cpr7, two closely related Hsp90-associated immunophilins from Saccharomyces cerevisiae, differ in their functional properties
:
C. Mayr, et al.; J. Biol. Chem.
275
, 34140 (2000)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P53691:
Peptidyl-prolyl cis-trans isomerase CPR6 (
Saccharomyces cerevisiae
(Baker's yeast))
General Literature References
Heat-shock protein 90, a chaperone for folding and regulation
:
D. Picard; Cell. Mol. Life Sci.
59
, 1640 (2002), (Review)
Abstract
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-202-035
Revised 28-Jun-07
