ALEXIS Biochemicals: Polyclonal Antibody to Repetin (AF646)

Advanced Search

You are here: Product Lines > Cell Differentiation

ALX-350-280

Revised 07-Oct-08

Xanthohumol
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Other Natural Products - DNA Regulation / Transcription

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-280-M005		5 mg	135.00 USD

Product Specification

FORMULA:	C₂₁H₂₂O₅
MW:	354.4
CAS NUMBER:	569-83-5, 6754-58-1
SOURCE/HOST:	Isolated from hops (Humulus lupulus).
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow to orange powder.
SOLUBILITY:	Soluble in DMSO (25mg/ml), methanol (10mg/ml) or 100% ethanol; soluble in aqueous buffers up to 20μM.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable as powder for at least 6 months when stored at +4°C in the dark. Stock solution made in DMSO should not be stored for longer than one month. Working solution is stable for 24 hours at 37°C and up to one week when stored at +4°C. Unstable in acidic (pH < 4.5) and alkaline (pH > 7.5) systems.
IDENTITY:	Identity determined by NMR.

Product Description

Prenylated chalcone found in beer. Potent inhibitor of diacylglycerol acetyltransferase (DGAT). Inhibits DNA polymerase and induces cell differentiation. Has antiproliferative and cytotoxic effects in human cancer cell lines. Inhibits human P450 enzymes. Induces quinone reductase. Inhibits the expression of HIF-1α and VEGF under hypoxic conditions. Induces apoptosis in mature adipocytes.

Product Specific Literature References

Xanthohumols, diacylglycerol acyltransferase inhibitors, from Humulus lupulus: N. Tabata, et al.; Phytochemistry 46, 683 (1997) Abstract
Antiproliferative and cytotoxic effects of prenylated flavonoids from hops (Humulus lupulus) in human cancer cell lines: C.L. Miranda, et al.; Food Chem. Toxicol. 37, 271 (1999) Abstract
In vitro inhibition of human P450 enzymes by prenylated flavonoids from hops, Humulus lupulus: M.C. Henderson, et al.; Xenobiotica 30, 235 (2000) Abstract
Prenylated chalcones and flavanones as inducers of quinone reductase in mouse Hepa 1c1c7 cells: C.L. Miranda, et al.; Cancer Lett. 149, 21 (2000) Abstract
Cancer chemopreventive activity of Xanthohumol, a natural product derived from hop: C. Gerhauser, et al.; Mol. Cancer Ther. 1, 959 (2002) Abstract
Xanthohumol and related prenylflavonoids from hops and beer: to your good health!: J.F. Stevens & J.E. Page; Phytochemistry 65, 1317 (2004), (Review) Abstract
Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis: J.Y. Yang, et al.; Apoptosis 12, 1953 (2007) Abstract

Further Categories Containing This Product:

Active Substances from Fruit and Vegetables • Estrogen & Estrogen Receptors / Related Products • Cell Differentiation • Hypoxia-inducible Factor [HIF] / Related Products • Chalcones • Natural Products - Apoptosis Inducers & Inhibitors • Antitumor Agents (Anti-proliferative) • Respiratory Chain Other Products • Obesity & Diabetes Other Products • Natural Products - Antitumor Reagents • VEGFs • Lipid Metabolism Other Products

ALX-420-045

Revised 02-Sep-08

Purmorphamine
SYNONYMS	2-(1-Naphthoxy)-6-(4-morpholinoanilino)-9-cyclohexylpurine
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Hedgehog [Hh] Signalling Pathway

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-420-045-M001		1 mg	40.00 USD
ALX-420-045-M005		5 mg	145.00 USD
ALX-420-045-M025		25 mg	510.00 USD

Product Specification

FORMULA:	C₃₁H₃₂N₆O₂
MW:	520.6
CAS NUMBER:	483367-10-8
PURITY:	≥98% (HPLC)
APPEARANCE:	Off-white to beige solid.
SOLUBILITY:	Soluble in DMSO (10mM).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING:	Protect from light. After reconstitution, prepare aliquots and store at -20°C.
HAZARD:	HARMFUL. MAY BE CARCINOGENIC. MAY BE TERATOGENIC.

Product Description

Cell permeable purine compound. Induces osteoblast differentiation. Agonist of smoothened by activation of the hedgehog pathway. Also shown to induce transdifferentiation in pre-adipocytes and myoblasts.

Product Specific Literature References

A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells: X. Wu, et al.; JACS 124, 14520 (2002) Abstract
Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway: X. Wu, et al.; Chem. Biol. 11, 1229 (2004) Abstract
Purmorphamine enhances osteogenic activity of human osteoblasts derived from bone marrow mesenchymal cells: M.M. Beloti, et al.; Cell Biol. Int. 29, 537 (2005) Abstract
Purmorphamine activates the Hedgehog pathway by targeting Smoothened: S. Sinha & J.K. Chen; Nat. Chem. Biol. 2, 29 (2006) Abstract

Further Categories Containing This Product:

Cell Differentiation

ALX-201-242

Revised 07-Aug-06

LIF (mouse) (recombinant)
SYNONYMS	Leukemia Inhibitory Factor (mouse) (recombinant)
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Differentiation

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-242-L001		1 ml	280.00 USD

Product Specification

MW:	~20kDa
SOURCE/HOST:	Produced in E. coli.
QUANTITY:	10⁷U
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.5% BSA. Contains no preservatives.
BIOLOGICAL ACTIVITY:	Induces macrophage differentiation and proliferation of hematopoietic stem cells; maintains indifferentiated state of of embryonic stem (ES) cell cultures; inhibits proliferation of the mouse myeloid cell line M1.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 6 months when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells: R.L. Williams, et al.; Nature 336, 684 (1988) Abstract
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells: C.C. Shih, et al.; Blood 95, 1957 (2000) Abstract
Leukemia inhibitory factor reduces body fat mass in ovariectomized mice: J.O. Jansson, et al.; Eur. J. Endocrinol. 154, 349 (2006) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Maintenance of ES Cells
1’000U/ml are recommended to maintain indifferentiated ES cells, R1 or E14 cells.
M1Growth Inhibition Assay:
50units/ml are defined as the concentration of LIF that induces 50% of differentiated cells in tissue culture medium.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-362

Revised 03-Apr-08

Syndecan-4 (human):Fc (human) (recombinant)
SYNONYMS	SYND4 (human):Fc (human) (recombinant) Amphiglycan (human):Fc (human) (recombinant) Ryudocan Core Protein (human):Fc (human) (recombinant)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Proteoglycans / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-362-C050		50 µg	350.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human syndecan-4 (aa 1-145) is fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT:	<1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P31431: Syndecan-4 (human) [Precursor]
MANUFACTURER	Manufactured by AdipoGen, Inc.

General Literature References

Syndecans-2 and -4; close cousins, but not identical twins: E.S. Oh and J.R. Couchman; Mol. Cells 17, 181 (2004) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Cell Differentiation

ALX-201-416

Revised 09-Jul-08

Dlk1 (mouse):Fc (human) (recombinant)
SYNONYMS	Delta-like Protein (DLK) (mouse):Fc (human) (recombinant) Pref-1 (mouse):Fc (human) (recombinant) Preadipocyte Factor 1 (mouse):Fc (human) (recombinant) Fetal Antigen 1 (FA1) (mouse):Fc (human) (recombinant)
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Adipokines Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-416-C010		10 µg	250.00 USD
ALX-201-416-C050		50 µg	570.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. The signal peptide and the extracellular domain of mouse Dlk1 (aa 1-305) is fused at the C-terminus to the Fc portion of human IgG.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT:	<1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q09163: DLK (mouse)
MANUFACTURER	Manufactured by AdipoGen, Inc.

Related Products

ALX-201-416

Dlk1 (mouse):Fc (human) (recombinant)

Further Categories Containing This Product:

Delta-like [DLL] & Jagged / Related Products • Recombinant Proteins / Fusion Proteins • Cell Differentiation

ALX-804-742

Revised 20-Nov-08

Monoclonal Antibody to Dlk1 (mouse) (PF105B)
SYNONYMS	anti-Delta-like Protein (DLK) (mouse) MAb (PF105B) anti-Pref-1 (mouse) MAb (PF105B) anti-Preadipocyte Factor 1 (mouse) MAb (PF105B) anti-Fetal Antigen 1 (FA1) (mouse) MAb (PF105B)
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Adipokines Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-742-C050		50 µg	195.00 USD
ALX-804-742-C100		100 µg	270.00 USD

Product Specification

CLONE:	PF105B
ISOTYPE:	Rat IgG2a
FORMULATION:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant mouse Dlk1 (extracellular domain).
SPECIFICITY:	Recognizes mouse Dlk1.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:10’000) Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for up to 6 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

Cell Differentiation • Monoclonal Antibodies • Delta-like [DLL] & Jagged / Related Products

ALX-804-743

Revised 20-Nov-08

Monoclonal Antibody to Dlk1 (mouse) (PF183E)
SYNONYMS	anti-Delta-like Protein (DLK) (mouse) MAb (PF183E) anti-Pref-1 (mouse) MAb (PF183E) anti-Preadipocyte Factor 1 (mouse) MAb (PF183E) anti-Fetal Antigen 1 (FA1) (mouse) MAb (PF183E)
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Adipokines Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-743-C050		50 µg	195.00 USD
ALX-804-743-C100		100 µg	270.00 USD

Product Specification

CLONE:	PF183E
ISOTYPE:	Rat IgG2a
FORMULATION:	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Recombinant mouse Dlk1 (extracellular domain).
SPECIFICITY:	Recognizes mouse Dlk1.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:10’000) Western Blot (1:2’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for up to 6 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

MANUFACTURER

Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

Cell Differentiation • Monoclonal Antibodies • Delta-like [DLL] & Jagged / Related Products

ALX-210-901

Revised 14-Jun-05

Polyclonal Antibody to SPRR2 (human)
SYNONYMS	anti-Small Proline-rich Protein 2 (human) PAb
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Differentiation

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-901-R100		100 µl	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Neat serum.
IMMUNOGEN:	Synthetic peptide corresponding to aa 30-47 (P³⁰KCPEPCPPPKCPQPCPP⁴⁷) of human SPRR2A (small proline-rich protein 2A) and SPRR2B.
SPECIFICITY:	Recognizes human SPRR2. Does not cross-react with SPRR1 or SPRR3.
APPLICATION:	Immunocytochemistry (1:200-1:500) Immunohistochemistry (paraffin sections (1:100)) Western Blot (1:1’000-1:4’000)
QUALITY CONTROL:	Western blot on differentiating human keratinocytes.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	After opening, prepare aliquots and store at -20°C. For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.

Product Images

Please click on thumbnails to enlarge.

Product Specific Literature References

The small proline-rich proteins constitute a multigene family of differentially regulated cornified cell envelope precursor proteins: D. Hohl, et al.; J. Invest. Dermatol. 104, 902 (1995) Abstract
Expression of small proline rich proteins in neoplastic and inflammatory skin diseases: M. De Heller-Milev et al.; Br. J. Dermatol. 143, 733 (2000) Abstract

General Information

The formation of the cornified (CE) envelope during the late stages of epidermal differentiation is essential for epidermal barrier function and protects the body against environmental attack and water loss. Formation of the cornified envelope involves the replacement of the plasma membrane by cross-linkage of precursor proteins such as loricrin, involucrin and small proline rich proteins (SPRR). SPRR genes constitute a multigene family clustered within the epidermal differentiation complex on chromosome 1q21. This cluster contains two SPRR1 genes, seven SPRR2 genes and a single SPRR3 gene. SPRR gene products are differentially expressed in vivo and in vitro suggesting that the SPRR multigene family evolved to provide highly specialized cornified envelope precursor proteins in stratified epithelia. SPRR2 is mainly expressed in interfollicular epidermis.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Towards a better classification of erythrokeratodermias: D. Hohl; Br. J. Dermatol. 143, 1133 (2000) Abstract

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P80370: DLK (human)
MANUFACTURER	Manufactured by AdipoGen, Inc.