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RANK & RANKL / OPG / Related Products
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Items 19 of 19
ALX-210-396 Revised 27-Mar-08
Polyclonal Antibody to RANKL (human)
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SYNONYMS anti-ODF (human) PAb
anti-OPGL (human) PAb
anti-TRANCE (human) PAb
anti-TNFSF 11 (human) PAb
anti-CD254 (human) PAb
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-396-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human RANKL.
SPECIFICITY: Recognizes human RANKL. Detects a band of ~35kDa band by Western blot.
APPLICATION: ELISA (direct and indirect: 1:2'000-1:10'000).
Flow Cytometry (1:500-1:1'000).
Western Blot (1:2'000-1:5'000 using ECL).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Polyclonal Antibodies • CD Antibodies
 
 
ALX-210-421 Revised 30-Oct-06
Polyclonal Antibody to RANK (ectodomain) (human)
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SYNONYMS anti-TRANCE-R (ectodomain) (human) PAb
anti-ODFR (ectodomain) (human) PAb
anti-TNFRSF 11A (ectodomain) (human) PAb
anti-CD265 (ectodomain) (human) PAb
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-210-421-C050   50 µg 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Contains no preservatives.
IMMUNOGEN: Recombinant human RANK.
SPECIFICITY: Recognizes human RANK. Detects a band of ~24kDa by Western blot.
APPLICATION: Flow Cytometry
Western blot (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Polyclonal Antibodies • CD Antibodies
 
 
ALX-210-945 Revised 20-Jun-08
Polyclonal Antibody to Osteoprotegerin (human) (AT121)
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SYNONYMS anti-OPG (human) MAb (AT121)
anti-TNFRSF 11B (human) MAb (AT121)
anti-Osteoclastogenesis Inhibitory Factor (human) MAb (AT121)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-945-C100   100 µg 310.00 USD Add To Cart
ALX-210-945B-C100 Biotin 100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human osteoprotegerin (OPG) cysteine-rich region (aa 22-202) (Prod. No. ALX-522-007).
SPECIFICITY: Recognizes human OPG.
APPLICATION: ELISA
Immunoprecipitation (1:200)
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
RANKL, RANK, osteoprotegerin: key partners of osteoimmunology and vascular diseases: M. Baud’huin, et al.; Cell. Mol. Life Sci. 64, 2334 (2007) Abstract
Related Products
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-370-002 Revised 19-Sep-06
Dexamethasone
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SYNONYMS 9α-Fluoro-16α-methylprednisolone
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-370-002-M250   250 mg 22.00 USD Add To Cart
ALX-370-002-G001   1 g 45.00 USD Add To Cart
Product Specification
FORMULA: C22H29FO5
MW: 392.5
CAS NUMBER: 50-02-2
MERCK INDEX: 14: 2943
RTECS: TU3980000
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or methanol. Slightly soluble in acetone or chloroform. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.
HAZARD: HARMFUL. MAY BE TERATOGENIC.

Product Description
Inhibits constitutive OPG mRNA steady-state levels in a dose- and time-dependent fashion, promoting osteoclast formation. Glucocorticoid with anti-inflammatory activity. Inhibits the induction of nitric oxide synthases (NOS).
Product Specific Literature References
Inhibition of the induction of nitric oxide synthase by glucocorticoids: yet another explanation for their anti-inflammatory effects?: S. Moncada & R.M.J. Palmer; TIPS 12, 130 (1991), (Review) Abstract
Glucocorticoids inhibit the induction of nitric oxide synthase and the related cell damage in adenocarcinoma cells: K.J. O'Connor & S. Moncada; Biochim. Biophys. Acta 1097, 227 (1991) Abstract
Nitric oxide mediates tumor necrosis factor-alpha cytotoxicity in endothelial cells: C. Estrada, et al.; BBRC 186, 475 (1992) Abstract
Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium: R.R. Dudek, et al.; BBRC 202, 1120 (1994) Abstract
Induction of the alkyltransferase (MGMT) gene by DNA damaging agents and the glucocorticoid dexamethasone and comparison with the response of base excision repair genes: T. Grombacher, et al.; Carcinogenesis 17, 2329 (1996) Abstract
Activation of human O6-methylguanine-DNA methyltransferase gene by glucocorticoid hormone: T. Biswas, et al.; Oncogene 18, 525 (1999) Abstract
Dexamethasone-induced thymocyte apoptosis: apoptotic signal involves the sequential activation of phosphoinositide-specific phospholipase C, acidic sphingomyelinase, and caspases: M.G. Cifone, et al.; Blood 93, 2282 (1999) Abstract; Full Text
Dexamethasone induces apoptosis in human T cell clones expressing low levels of Bcl-2: M.S. Montani, et al.; Cell Death Differ 6, 79 (1999) Abstract
Dexamethasone inhibits the phosphorylation of retinoblastoma protein in the suppression of human vascular smooth muscle cell proliferation: T.D. Reil, et al.; J. Surg. Res. 92, 108 (2000) Abstract
Dexamethasone suppresses iNOS gene expression by inhibiting NF-kappaB in vascular smooth muscle cells: M. Matsumura, et al.; Life Sci. 69, 1067 (2001) Abstract
Dexamethasone inhibits inducible nitric-oxide synthase expression and nitric oxide production by destabilizing mRNA in lipopolysaccharide-treated macrophages: R. Korhonen, et al.; Mol. Pharmacol. 62, 698 (2002) Abstract; Full Text
Molecular mechanisms underlying dexamethasone inhibition of iNOS expression and activity in C6 glioma cells: J. Shinoda, et al.; Glia 42, 68 (2003) Abstract
Dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-kappaB activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line: A.L. Vital, et al.; Mediators Inflamm. 12, 71 (2003) Abstract
Dexamethasone suppresses eNOS and CAT-1 and induces oxidative stress in mouse resistance arterioles: S.C. Schafer, et al.; Am. J. Physiol. Heart Circ. Physiol. 288, H436 (2005) Abstract; Full Text
Related Products
Further Categories Containing This Product:
GITR & GITRL Other Products • RANK & RANKL / OPG Other Products • Antitumor Agents (Hormone-related) • NOS Inhibitors (NOS Induction & Enzyme Activity) • Chemopreventive Agents Other Products • DNA & RNA Methyl & Alkyl Transferases / Related Products • Cell Cycle Other Products
 
 
ALX-522-007 Revised 30-Jul-07
Osteoprotegerin (human):Fc (human) (recombinant)
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SYNONYMS OPG (human):Fc (human) (recombinant)
TNFRSF 11B (human):Fc (human) (recombinant)
Osteoclastogenesis Inhibitory Factor (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY OPG
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ALX-522-007-C050   50 µg 340.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region of human OPG (osteoprotegerin) (aa 22-202) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human and mouse RANKL and TRAIL.
BIOLOGICAL ACTIVITY: Inhibits rhsTRAIL-mediated apoptosis at a concentration range of 5-20µg/ml, as well as rhsRANKL-induced osteoclastogenesis and stimulation of dendritic cells. Concentrations of rhOPG:Fc required to inhibit may vary depending on the cell type studied and on the concentration of rhsTRAIL used to kill the cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
A CD40-CD95L fusion protein interferes with CD40L-induced prosurvival signaling and allows membrane CD40L-restricted activation of CD95: C. Assohou-Luty, et al.; J. Mol. Med. 84, 785 (2006) Abstract
General Information
Osteoprotegerin (OPG) is a secreted tumor necrosis factor receptor homologue that inhibits osteoclastogenesis and increases bone density in vivo. OPG contains 4 cysteine-rich repeats and 2 ‘death domains’ (DD). OPG binds to RANKL (TRANCE) and with a similar affinity to TRAIL.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O00300: Osteoprotegrin (human) (precursor)
AfCS Signalling Gateway link A002819: Osteoprotegrin (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-012 Revised 28-Apr-06
RANKL, Soluble (human) (recombinant)
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SYNONYMS ODF, Soluble (human) (recombinant)
OPGL, Soluble (human) (recombinant)
TRANCE, Soluble (human) (recombinant)
TNFSF 11, Soluble (human) (recombinant)
CD254, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-522-012-C010   10 µg 365.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~18kDa (non-glycosylated), ~28kDa (glycosylated) (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human RANKL (aa 151-316) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
SOLUBILITY: Soluble in water.
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse RANK.
BIOLOGICAL ACTIVITY: Supports the survival of dendritic cells and osteoclasts.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Specific Literature References
Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract
A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004) Abstract
General Information
RANK ligand (RANKL), also called TRANCE, OPGL and ODF, is a member of the TNF family that is expressed in activated T cells, lymph nodes and in stromal cell lines. RANKL (TRANCE) interacts with its receptor RANK expressed on mature dendritic cells (DC) and mature osteoclasts, leading to the inhibition of apoptosis, probably through the upregulation of bcl-x. RANKL is, therefore, a useful tool for enhancing DC and osteoclast survival and activity. Osteoprotegerin (OPG, see Prod. No. ALX-522-007) is a potent inhibitor of RANKL.
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O014788: RANKL (human)
AfCS Signalling Gateway link A002020: RANKL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function: D.M. Anderson, et al.; Nature 390, 175 (1997) Abstract
TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor: B.R. Wong, et al.; J. Exp. Med. 186, 2075 (1997) Abstract
Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation: D.L. Lacey, et al.; Cell 93, 165 (1998) Abstract
Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL: H. Yasuda, et al.; PNAS 95, 3597 (1998) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-039 Revised 24-Mar-05
RANK (human):Fc (human) (recombinant)
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SYNONYMS TRANCE-R (human):Fc (human) (recombinant)
ODFR (human):Fc (human) (recombinant)
TNFRSF 11A (human):Fc (human) (recombinant)
CD265 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-039-C050   50 µg 360.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~55kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region of human RANK (aa 29-213) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds human and mouse RANKL.
BIOLOGICAL ACTIVITY: Inhibits human rhsRANKL-induced survival of dendritic cells and osteoclasts.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C. Once reconstituted, it is recommended to prepare appropriate aliquots and to store them at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
The receptor activator of NF-κB (RANK) is a member of the tumor necrosis factor receptor (TNF-R) family. RANK is a type I transmembrane receptor. It is 616 aa long and contains 4 cysteine-rich motifs in its extracellular domain, while its long cytoplasmic domain spans 383 aa. RANK is mainly expressed on dendritic cells. After binding its ligand RANKL, RANK interacts with TNF receptor-associated factor 1 (TRAF1), TRAF2, TRAF3, TRAF5 and TRAF6 which mediate activation of nuclear factor NF-κB and JNK. RANK-RANKL interactions promote survival of dendritic cells as well as differentiation in osteoclasts.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9Y6Q6: RANK (human) (precursor)
AfCS Signalling Gateway link A002019: RANK (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-131 Revised 27-Mar-08
Fc (human):RANKL, Soluble (mouse) (rec.)
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SYNONYMS Fc (human):ODF, Soluble (mouse) (recombinant)
Fc (human):OPGL, Soluble (mouse) (recombinant)
Fc (human):TRANCE, Soluble (mouse) (recombinant)
Fc (human):TNFSF 11, Soluble (mouse) (recombinant)
Fc (human):CD254, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-131-C010   10 µg 390.00 USD Add To Cart
Product Specification
MW: ~55kDa
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse RANKL (aa 157-316) is fused to the Fc portion of human IgG1.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to mouse RANK.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O35235: TNF11 (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-550-516 Revised 21-May-08
Compound A
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SYNONYMS CpdA
2-((4-Acetophenyl)-2-chloro-N-methyl)ethylammonium chloride