• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
Catenin / Related Products
You are here: Product Lines > Cytoskeleton > Catenin / Related Products
Toolbar - View Selection
 
Items 8 of 8
ALX-804-105 Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (Core) (9G10)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-105-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 9G10
ISOTYPE: Mouse IgG2b
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Recombinant human β-catenin.
SPECIFICITY: Recognizes the core region (armadillo repeats; epitope recognized: aa 422-685) of human, mouse, rat (weak) and dog β-catenin.
APPLICATION: Immunocytochemistry (0.1-1µg/ml)
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot: (1µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-027)
Product Specific Literature References
beta-Catenin and plakoglobin N- and C-tails determine ligand specificity: G. Solanas, et al.; J. Biol. Chem. 279, 49849 (2004) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-260/1 Revised 21-Oct-08
Monoclonal Antibody to β-Catenin (8E4)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-260/1-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 8E4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute in 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to human β-catenin aa 27-37.
SPECIFICITY: Recognizes human, mouse, rat (weak) and dog β-catenin. Recognizes an epitope between aa 27-37 and an additional epitope in the C-Terminal region (see [5]).
Note: For specific detection of dephospho-β-catenin use Prod. No. ALX-804-259.
APPLICATION: Flow Cytometry (see Lit. Ref. [3])
Immunocytochemistry (see Lit. Ref. [4])
Immunohistochemistry (paraffin sections) (see Lit. Ref. [3])
Western Blot: 0.5µg/ml for HRPO/ECL detection. Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
BSA may be used alternatively but milk is not recommended for blocking and dilution!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL: Included (Prod. No. ALX-840-022).
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[2] Sulindac targets nuclear beta-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines: E.M. Boon, et al.; Br. J. Cancer 90, 224 (2004) Abstract
[1] Activation of the canonical beta-catenin pathway by histamine: S.H. Diks, et al.; J. Biol. Chem. 278, 52491 (2003) Abstract; Full Text
[3] Illegitimate WNT signaling promotes proliferation of multiple myeloma cells: P.W. Derksen, et al.; PNAS 101, 6122 (2004) Abstract; Full Text
[4] Modulation of intracellular beta-catenin localization and intestinal tumorigenesis in vivo and in vitro by sphingolipids: E.M. Schmelz, et al.; Cancer Res. 61, 6723 (2001) Abstract
[5] Wnt signaling and phosphorylation status of beta-catenin: importance of the correct antibody tools: M. van Noort, et al.; Blood 110, 2778 (2007) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-101 Revised 04-Jun-08
Monoclonal Antibody to α-Catenin (15D9)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-101-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: 15D9
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Recombinant human α-catenin.
SPECIFICITY: Recognizes human, mouse, rat and dog α-catenin. Does not cross-react with β-catenins.
APPLICATION: Immunocytochemistry (0.1-1µg/ml; formaldehyde fixation is tolerated)
Immunoprecipitation
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Yes (Prod. No. ALX-840-026).
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-106 Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (CT) (10H8)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-106-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 10H8
ISOTYPE: Mouse IgG2a
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and Sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Recombinant human β-catenin.
SPECIFICITY: Recognizes the C-terminus of human, mouse, rat (weak) and dog β-catenin (aa 769-781).
APPLICATION: Immunocytochemistry (0.1-1µg/ml)
Immunoprecipitation
Western blot (0.1µg/ml for HRPO/ECL detection).
Recommended blocking buffer milk: 2x PBS, 5-8% (w/v) dry milk powder and 0.1% (v/v) Tween 20.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-027).
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-059 Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (CT/Exon 14) (7D8)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-059-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 7D8
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Recombinant human β-catenin.
SPECIFICITY: Recognizes the C-terminus (exon 14) of human, mouse, rat (weak) and a dog β-catenin.
APPLICATION: Immunocytochemistry (0.1-1µg/ml) 
Immunohistochemistry (paraffin sections) 
Immunoprecipitation
Western blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-027).
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-259 Revised 21-Oct-08
Monoclonal Antibody to β-Catenin (dephospho, aa 35-50) (7A7)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-259-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 7A7
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to aa 35-50 of human β-catenin.
SPECIFICITY: Recognizes human, mouse, rat (weak) and dog cytoplasmic dephospho β-catenin.
APPLICATION: Immunocytochemistry (1-10µg/ml)
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
BSA may be used alternatively but milk is not recommended for blocking and dilution!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-022).
Product Images
Please click on thumbnails to enlarge.
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-058 Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (Exon 3) (9G2)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-058-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 9G2
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Recombinant human β-catenin.
SPECIFICITY: Recognizes exon 3 (α-catenin-binding site) of human, mouse, rat (weak) and dog β-catenin.
APPLICATION: Immunocytochemistry (0.1-1µg/ml)
Immunoprecipitation
Western Blot (0.1µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone (PVP), 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-027)
Product Images
Please click on thumbnails to enlarge.
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-060 Revised 13-Jun-08
Monoclonal Antibody to β-Catenin (NT/Exon 2) (7D11)
Add to Clipboard
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Catenin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-060-C100   100 µg 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 7D11
ISOTYPE: Mouse IgG2a
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Recombinant human β-catenin.
SPECIFICITY: Recognizes the N-terminus (exon 2) of human, mouse, rat (weak) and dog β-catenin.
APPLICATION: Immunoprecipitation
Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL: Included (Prod. No. ALX-840-027).
Product Images
Please click on thumbnails to enlarge.
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
© 2008 ALEXIS Corporation, Privacy Policy, Disclaimer, Feedbackheader picture © micronaut.ch, another CS2 website

Catalogs / Brochures

E-Newsletter

 

Shopping Cart [0]

 

Distributors

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.