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ALX-201-057
Revised 30-Jun-08
Caspase-2 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-057-U025
25 U
203.00 USD
ALX-201-057-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.36
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥
95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
10'000-13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VDVAD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening capase inhibitors, studying enzyme regulation and kinetics, determining specificity of capase substrates, or as positive control in caspase activity assays.
We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-2 assay protocol, please refer to Caspase-2 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-214
or
ALX-850-213
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-2 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kDa) and small (12kDa) subunits which associate to form the active enzyme. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA (Prod. No.
ALX-260-059
). The signal can be detected by spectrophotometry.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P42575:
Caspase-2 (human) (precursor)
AfCS Siganlling Gateway link A000497:
Caspase-2 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-059
Revised 21-Jul-08
Caspase-3 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-059-U025
25 U
119.00 USD
ALX-201-059-U100
100 U
273.00 USD
Product Specification
EC:
3.4.22.56
SOURCE/HOST:
Produced in
E. coli
.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
300’000-400’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-
p
NA (Prod. No.
ALX-260-033
) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No.
ALX-850-216
or
ALX-850-215
).
QUALITY CONTROL:
Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD-
p
NA (Prod. No.
ALX-260-033
) and Ac-DEVD-AFC (Prod. No.
ALX-260-032
).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-3 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. It exists in cells as an inactive 32kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and granzyme B. The processed form of caspase-3 consists of large (17kDa) and small (11kDa) subunits which associate to form the active enzyme. Active caspase-3 has been shown involved in the proteolysis of several important molecules, such as poly(ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK) and others.
BACKGROUND/TECHNICAL INFORMATION
Swiss Prot link:
Caspase-3 (human) (precursor)
AfCS Signalling Gateway link:
Caspase-3 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-060
Revised 02-Jun-08
Caspase-6 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-060-U025
25 U
203.00 USD
ALX-201-060-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.59
SOURCE/HOST:
Produced in
E. coli.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
BIOLOGICAL ACTIVITY:
Cleaves substrates exhibiting consensus sequence VEID, e.g. Ac-VEID-AFC (Prod. No.
ALX-260-111
), Ac-VEID-AMC (Prod. No.
ALX-260-064
) and Ac-VEID-pNA (Prod. No.
ALX-260-063
).
SPECIFIC ACTIVITY:
~13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VEID-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-6 (also known as Mch2) is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-6 consists of large (18kDa) and small (11kDa) subunits which associate to form the active enzyme. The active caspase-6 has been shown involving in the proteolysis of poly(ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P55212:
Caspase-6 (human) (precursor)
AfCS Signalling Gateway link A000501:
Caspase-6 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-061
Revised 21-Jul-08
Caspase-7 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-061-U025
25 U
203.00 USD
ALX-201-061-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.60
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
~25’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-
p
NA
(p
NA:
p
-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P55210:
Caspase-7 (human) (precursor)
AfCS Signalling Gateway link A000502:
Caspase-7 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-062
Revised 04-Aug-08
Caspase-8 (active) (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Caspases
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-062-U025
25 U
203.00 USD
ALX-201-062-U100
100 U
371.00 USD
Product Specification
EC:
3.4.22.-
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:
5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
Caspase-8 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Same as other caspases, caspase-7 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed form of caspase-8 consists of large and small subunits which associate to form the active enzyme.
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q14790:
Caspase-8 (human) (precursor)
AfCS Signalling Gateway link A000503:
Caspase-8 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-063
Revised 25-Oct-06
PARP-1 (human) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-063-C020
20 µg
348.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
1mg/ml.
PURITY:
≥99% (SDS-PAGE
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. In 100mM TRIS-HCl, pH 7.5,containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:
≥600U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C, pH 7.5.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Overproduction and large-scale purification of the human poly(ADP- ribose) polymerase using a baculovirus expression system:
H. Giner, et al.; Gene
114
, 279 (1992)
Abstract
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing
:
M. Malanga & F.R. Althaus; J. Biol. Chem.
279
, 5244 (2004)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Related Products
ALX-840-040
DNA (purified) (DNAse I activated)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-064
Revised 25-Oct-06
PARP-2 (mouse) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-2 (mouse) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-2
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-064-C020
20 µg
368.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
~1mg/ml
PURITY:
≥98% (SDS-PAGE)
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. In 100mM TRIS-HCl, pH 7.5, containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:
≥380U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min at 25°C, pH 7.5. See also separate protocol (link below).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase:
J.C. Ame, et al.; J. Biol. Chem.
274
, 17860 (1999)
Abstract
;
Full Text
A bidirectional promoter connects the poly(ADP-ribose) polymerase 2 (PARP-2) gene to the gene for RNase P RNA. structure and expression of the mouse PARP-2 gene:
J.C. Ame, et al.; J. Biol. Chem.
276
, 11092 (2001)
Abstract
;
Full Text
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing
:
M. Malanga & F.R. Althaus; J. Biol. Chem.
279
, 5244 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link O88554:
PARP-2 (mouse)
Related Products
ALX-840-040
DNA (purified) (DNAse I activated)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-065
Revised 18-Sep-08
MGMT (human) (recombinant)
SYNONYMS
O
6
-Methylguanine-DNA Methyltransferase (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Repair Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-065-R050
50 µl
320.00 USD
Product Specification
EC:
2.1.1.63
SOURCE/HOST:
Produced in
E. coli
. Full length human MGMT is fused at the N-terminus to a GST-tag.
PURITY:
Purified with Glutathiose-Sepharose 4B and thrombin cleavage
FORMULATION:
Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA.
SPECIFIC ACTIVITY:
100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O
6
-methylguanine from DNA.
QUALITY CONTROL:
The biological activity has been checked in a radioactive standard MGMT assay.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Do not store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P16455:
MGMT (human)
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products
•
Recombinant Proteins / Fusion Proteins
ALX-201-069
Revised 08-Jun-06
Nitric Oxide Synthase (Inducible) (human) (recombinant)
SYNONYMS
iNOS (human) (recombinant)
NOS II (human) (recombinant)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
NOS
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-069-R150
150 µl
320.00 USD
Product Specification
MW:
~130kDa/subunit; homodimer.
EC:
1.14.13.39
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
>5mg/ml.
PURITY:
50’000 x g supernatant.
FORMULATION:
Liquid. In 50mM triethanolamine, pH 7.5, containing 0.5mM EDTA, 7mM GSH, 0.2% CHAPSO, 1µM leupeptin, 1µM pepstatin A, 1µM E64, 50µM L-arginine, 1mg/ml trypsin inhibitor and 10% glycerol.
SPECIFIC ACTIVITY:
≥0.015nmol/µl/min. Amount assayed 1µl. Optimal conditions: 37°C, pH 7.0, 1mM L-arginine, 1mM NADPH, 10µM (6R)-tetrahydrobiopterin, 5µM FAD, 5µM FMN and 7mM GSH.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
USE/STABILITY:
iNOS is relatively unstable. Keep stock vial on ice at all times when performing experiments! Enzyme loses approx. 10-15% of activity during a single freeze/thaw cycle.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Endothelial nitric-oxide synthase (type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein kinases:
E. Butt, et al.; J. Biol. Chem.
275
, 5179 (2000)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P35228:
NOS II (human)
AfCS Signalling Gateway link A001659:
NOS II (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-070
Revised 10-Feb-05
Nitric Oxide Synthase (Endothelial) (human) (recombinant)
SYNONYMS
eNOS (human) (recombinant)
NOS III (human) (recombinant)
PRODUCT LINE
Nitric Oxide Pathway
PRODUCT CATEGORY
NOS
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-070-R100
100 µl
320.00 USD
Product Specification
MW:
~130kDa/subunit; homodimer.
EC:
1.14.13.39
SOURCE/HOST:
Produced in Sf9 cells.
