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Intermediate Filaments / Related Products
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ALX-804-640 Revised 27-Mar-08
Monoclonal Antibody to Cytokeratin 18 (human) (M5)
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SYNONYMS anti-CK18 (human) MAb (M5)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytokeratin-based Apoptosis Detection
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-640-T200   200 tests 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: M5
ISOTYPE: Mouse IgG2b
CONCENTRATION: 0.1mg/ml
FORMULATION: Liquid. In PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
IMMUNOGEN: CK18 (cytokeratin 18) fragments purified from human carcinomas.
SPECIFICITY: Recognizes an internal, formalin-resistant epitope (aa 284-396) present on uncleaved as well as caspase-cleaved human CK18 fragments.
APPLICATION: Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Western Blot
Note: A dilution of 1:100 in incubation buffer is generally recommended. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
BACKGROUND/TECHNICAL INFORMATION For more information on procedures please also see the Original Manufacturer’s Product Description (click here to download pdf).
MANUFACTURER Manufactured by Peviva AB.
General Literature References
Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1751 (2004) Abstract; Full Text
Docetaxel induces apoptosis in hormone refractory prostate carcinomas during multiple treatment cycles: G. Kramer, et al.; Br. J. Cancer 94, 1592 (2006) Abstract
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • Keratins / Related Products • Tumor Markers
 
 
ALX-804-641 Revised 27-Mar-08
Monoclonal Antibody to Cytokeratin 18 (M6)
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SYNONYMS anti-CK18 MAb (M6)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Cytokeratin-based Apoptosis Detection
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-641-T200   200 tests 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
CLONE: M6
ISOTYPE: Mouse IgG2b
CONCENTRATION: 0.1mg/ml
FORMULATION: Liquid. In PBS containing 0.1% BSA, PEG, sucrose and 0.09% sodium azide.
IMMUNOGEN: CK18 (cytokeratin 18) fragments purified from human carcinomas.
SPECIFICITY: Recognizes an internal, formalin-resistant epitope (aa 284-396) present on uncleaved as well as caspase-cleaved human, mouse, rat and dog CK18 fragments.
APPLICATION: Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Western Blot
Note: A dilution of 1:200 in incubation buffer is generally recommended. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
BACKGROUND/TECHNICAL INFORMATION For more information on procedures please also see the Original Manufacturer’s Product Description (click here to download pdf).
MANUFACTURER Manufactured by Peviva AB.
General Literature References
Differentiation between cell death modes using measurements of different soluble forms of extracellular cytokeratin 18: G. Kramer, et al.; Cancer Res. 64, 1715 (2004) Abstract; Full Text
Docetaxel induces apoptosis in hormone refractory prostate carcinomas during multiple treatment cycles: G. Kramer, et al.; Br. J. Cancer 94, 1592 (2006) Abstract
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • Keratins / Related Products • Tumor Markers
 
 
ALX-804-672 Revised 20-Nov-08 New product
Monoclonal Antibody to Lamin A/C (4C11)
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Laminin / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-672-C200   200 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 4C11
ISOTYPE: Mouse IgG2a
CONCENTRATION: ~1mg/ml
FORMULATION: Liquid. Hybridoma supernatant containing sodium azide.
IMMUNOGEN: Recombinant human lamin A/C (aa 430-545).
SPECIFICITY: Recognizes human, mouse, rat, hamster and monkey lamin A/C. Cross-reacts with lamin B1 and lamin B2.
APPLICATION: Immunocytochemistry (1:100)
Immunoprecipitation (5-10µl/assay)
Western Blot (1:2’000-1:5’000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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General Information
Lamin A is a type V intermediate filament protein, involved in maintaining nuclear shape and structure, transcriptional regulation, nuclear pore positioning and function, and heterochromatin organization. Mutations in lamin A are associated with several genetic degenerative disease, including Hutchinson-Gilford progeria syndrome (HGPS), which is caused by Progerin a truncated version of lamin A. Recently, A-type lamins were described as novel prognostic biomarkers for colorectal cancer, and lamin A (but not lamin C) is a potential adult stem cell biomarker in colonic crypts.
General Literature References
The mutant form of lamin A that causes Hutchinson-Gilford progeria is a biomarker of cellular aging in human skin: D. McClintock, et al.; PLoS ONE 2, e1269 (2007) Abstract; Full Text
Lamin A/C, laminopathies and premature ageing: B. Liu & Z. Zhou; Histol. Histopathol. 23, 747 (2008) Abstract
Lamin A-dependent misregulation of adult stem cells associated with accelerated ageing: P. Scaffidi & T. Misteli; Nat. Cell Biol. 10, 452 (2008) Abstract; Full Text
Lamin A/C is a risk biomarker in colorectal cancer: N.D. Willis, et al.; PLoS ONE 3, e2988 (2008) Abstract; Full Text
Related Products
Further Categories Containing This Product:
Intermediate Filaments Other Products • Monoclonal Antibodies • Nuclear Envelope & Nuclear Transport
 
 
ALX-804-662 Revised 15-Apr-08
Monoclonal Antibody to Progerin (human) (13A4)
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SYNONYMS anti-Δ50 Lamin A (human) MAb (13A4)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Intermediate Filaments Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-662-R200   200 µl 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 13A4
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Purified supernatant containing 0.02% sodium azide.
IMMUNOGEN: Synthetic KLH-coupled peptide corresponding to aa 604-611 (G604AQSPQNC611) at the C-terminus of human progerin.
SPECIFICITY: Recognizes human progerin. Does not cross-react with endogenous prelamin A.
APPLICATION: Immunocytochemistry (1:10)
Immunoprecipitation (20µl/assay)
Western Blot (1:1000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q6UYC3: human Progerin
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • Nuclear Envelope & Nuclear Transport
 
 
ALX-350-153 Revised 28-Jul-08
Withaferin A
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SYNONYMS 5,6-Epoxy-4,27-dihydroxy-1-oxowitha-2,24-dienolide
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Antitumor Reagents
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-153-M001   1 mg 60.00 USD Add To Cart
ALX-350-153-M005   5 mg 240.00 USD Add To Cart
Product Specification
FORMULA: C28H38O6
MW: 470.6
CAS NUMBER: 5119-48-2
MERCK INDEX: 14: 10048
RTECS: KE7288500
SOURCE/HOST: Isolated from Withania somnifera.
PURITY: ≥94% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO (20 mg/ml), methanol (10 mg/ml) or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
HAZARD: MAY BE MUTAGENIC. MAY BE CARCINOGENIC. HARMFUL.

Product Description
Cell permeable and potent angiogenesis inhibitor from the family of withanolides. Inhibits endothelial cells (HUVEC) sprouting in vitro (IC50=12nM) and in vivo. Exhibits antitumor, anti-inflammatory, radiosensitizing, and immunosuppressive properties. Potently inhibits NF-κB activation by preventing the TNF-induced activation of IκB kinase β (IKKβ). Affects AP1 transcription and induces cell death. Alters cytoskeletal architecture by covalently binding annexin II and stimulating its basal F-actin cross-linking activity which inhibits the migratory and invasive capability of endothelial cells. Displays neuronal regenerative properties. Targets the intermediate filament protein vimentin.
Product Specific Literature References
Tumor inhibitors. XXXIX. Active principles of Acnistus arborescens. Isolation and structural and spectral studies of withaferin A and withacnistin: S.M. Kupchan, et al.; J. Org. Chem. 34, 3858 (1969) Abstract
In vivo growth inhibitory and radiosensitizing effects of withaferin A on mouse Ehrlich ascites carcinoma: P.U. Devi, et al.; Cancer Lett. 95, 189 (1995) Abstract
Growth inhibition of human tumor cell lines by withanolides from Withania somnifera leaves: B. Jayaprakasam, et al.; Life Sci. 74, 125 (2003) Abstract
Withaferin A is a potent inhibitor of angiogenesis: R. Mohan, et al.; Angiogenesis 7, 115 (2004) Abstract
Neuritic regeneration and synaptic reconstruction induced by withanolide: A: T. Kuboyama, et al.; Br. J. Pharmacol. 144, 961 (2005) Abstract; Full Text
Actin microfilament aggregation induced by withaferin A is mediated by annexin II: R.R. Falsey, et al.; Nat. Chem. Biol. 2, 33 (2006) Abstract
Withaferin a strongly elicits IkappaB kinase beta hyperphosphorylation concomitant with potent inhibition of its kinase activity: M. Kaileh, et al.; J. Biol. Chem. 282, 4253 (2007) Abstract; Full Text
The tumor inhibitor and antiangiogenic agent withaferin A targets the intermediate filament protein vimentin: P. Bargagna-Mohan, et al.; Chem. Biol. 14, 623 (2007) Abstract
Further Categories Containing This Product:
Natural Products - Immunomodulators • Natural Products for Angiogenesis Research • Natural Products - NF-kB Pathway Inhibitors • Natural Products for Cytoskeletal Research • Antitumor Agents (Apoptosis Inducers) • Vimentin / Related Products • Natural Products - Anti-inflammatory Agents • NF-kB Pathway Inhibitors
 
 
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