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Monoclonal Antibodies
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ALX-804-807 Revised 31-Aug-06
Monoclonal Antibody to BAFF-R (human) (11C1)
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SYNONYMS anti-BR3 (human) MAb (11C1)
anti-BlySR3 (human) MAb (11C1)
anti-TNFRSF 13C (human) MAb (11C1)
anti-CD268 (human) MAb (11C1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF-R
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-807-C100   100 µg 330.00 USD Add To Cart
ALX-804-807TD-T100 ATTO 488 100 tests 380.00 USD Add To Cart
ALX-804-807TS-T100 ATTO 647N 100 tests 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 11C1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated mouse hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Mouse B cell lymphoma L1.2 cells expressing human BAFF-R.
SPECIFICITY: Recognizes human BAFF-R.
APPLICATION: Flow Cytometry: works on endogenous.
Immunohistochemistry (frozen sections; paraffin sections): works on endogenous.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored as recommended.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.
Product Images
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Product Specific Literature References
B cell-activating factor belonging to the TNF family (BAFF)-R Is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells: L.G. Ng, et al.; J. Immunol. 173, 807 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION 1 test means: 1µl of MAb is used to stain 500’000 cells in a sample volume of 50µl.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-824 Revised 31-Oct-06
Monoclonal Antibody to BAFF-R (mouse) (7H22-E16)
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SYNONYMS anti-BR3 (mouse) MAb (7H22-E16)
anti-BlySR3 (mouse) MAb (7H22-E16)
anti-TNFRSF 13C (mouse) MAb (7H22-E16)
anti-CD268 (mouse) MAb (7H22-E16)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF-R
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-824-C100   100 µg 320.00 USD Add To Cart
ALX-804-824TD-T100 ATTO 488 100 tests 380.00 USD Add To Cart
ALX-804-824TS-T100 ATTO 647N 100 tests 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 7H22-E16
ISOTYPE: Rat IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE).
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse BAFF-R extracellular domain (aa 2-70).
SPECIFICITY: Recognizes mouse BAFF-R.
APPLICATION: Flow Cytometry (1:100)
Western Blot (1:500)
Note: Not recommended for immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells: L.G. Ng, et al.; J. Immunol. 173, 807 (2004) Abstract
CD45 links the B cell receptor with cell survival and is required for the persistence of germinal centers: N.D. Huntington, et al.; Nat. Immunol. 7, 190 (2006) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION 1 test means: 1µl of MAb is used to stain 500’000 cells in a sample volume of 50µl.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The BAFF/APRIL system: an important player in systemic rheumatic diseases: F. Mackay, et al.; Curr. Dir. Autoimmun. 8, 243 (2005) Abstract
Further Categories Containing This Product:
Monoclonal AntibodiesCD Antibodies
 
 
ALX-803-323 Revised 27-May-08
Monoclonal Antibody to BAG-3 (AC-1)
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SYNONYMS anti-Bcl-2-binding Athanogene-3 MAb (AC-1)
anti-Docking Protein CAIR-1 MAb (AC-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-803-323-C100   100 µg 280.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: AC-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 18-33 (D18RDPLPPGWEIKIDPQ33) of N-terminal human BAG-3 (Bcl-2-binding athanogene 3).
SPECIFICITY: Recognizes human, mouse and rat BAG-3.
APPLICATION: ELISA
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Bag3 gene expression is regulated by heat shock factor 1: S. Franceschelli, et al.; J. Cell Physiol. 215, 575 (2008) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-3 (CAIR-1; Bis) forms a complex with HSC70 and assists in polypeptide folding. In addition, this complex can mediate protein delivery to proteasome and is able to downregulate apoptosis by interfering with cytochrome c release, apoptosome assembly and other events. In human cancer cell lines, BAG-3 expression sustains cell survival and underlies resistance to therapy through downmodulation of apoptosis.
General Literature References
BAG3 protein controls B-chronic lymphocytic leukaemia cell apoptosis: M.F. Romano, et al.; Cell Death Differ. 10, 383 (2003) Abstract; Full Text
Apoptosis inhibition in cancer cells: a novel molecular pathway that involves BAG3 protein: A. Rosati, et al.; Int. J. Biochem. Cell Biol. 39, 1337 (2007) Abstract
 
 
ALX-804-601 Revised 20-Jun-08
Monoclonal Antibody to BAP31 (A1/182)
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SYNONYMS anti-BCR-associated Protein BAP31 MAb (A1/182)
anti-p28 BAP31 MAb (A1/182)
anti-CDM MAb (A1/182)
anti-DXS1357E MAb (A1/182)
anti-6C6-AG Tumor-associated Antigen MAb (A1/182)
PRODUCT LINE Immunology
PRODUCT CATEGORY B Cell Receptor [BCR] & Co-Receptors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-601-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: A1/182
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml after reconstitution.
FORMULATION: Lyophilized. Protein G-affinity purified and gel filtrated (FPLC).
RECONSTITUTION: Reconstitute with 100µl PBS containing 0.1% sodium azide.
IMMUNOGEN: BAP31 (BCR associated protein BAP031) from Vero monkey cells.
SPECIFICITY: Recognizes human and monkey BAP31.
APPLICATION: ELISA
Flow Cytometry
Immunocytochemistry (1:1’000)
Immunohistochemistry (frozen sections)
Immunoprecipitation (2µl)
Western Blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment: J. Klumperman, et al.; J. Cell Sci. 111, 3411 (1998) Abstract; Full Text
A novel direct interaction of endoplasmic reticulum with microtubules: D.R. Klopfenstein, et al.; EMBO J. 17, 6168 (1998) Abstract; Full Text
Subdomain-specific localization of CLIMP-63 (p63) in the endoplasmic reticulum is mediated by its luminal alpha-helical segment: D.R. Klopfenstein, et al.; J. Cell Biol. 153, 1287 (2001) Abstract; Full Text
Proteomics of endoplasmic reticulum-golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46: L. Breuza, et al.; J. Biol. Chem. 279, 47242 (2004) Abstract; Full Text
Capturing protein interactions in the secretory pathway of living cells: B. Nyfeler, et al.; PNAS 102, 6350 (2005) Abstract; Full Text
The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi: S. Mitrovic, et al.; Mol. Biol. Cell 19, 1976 (2008) Abstract
General Information
BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca2+-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2.
General Literature References
Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol. 20, 6731 (2000) Abstract; Full Text
Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol: D.G. Breckenridge, et al.; J. Cell. Biol. 160, 1115 (2003) Abstract; Full Text
Bap31 Is an Itinerant Protein that Moves between the Peripheral ER and a Juxtanuclear Compartment Related to ER-associated Degradation: Y. Wakana, et al.; Mol. Biol. Cell 19, 1825 (2008) Abstract; Full Text
 
 
ALX-804-812 Revised 20-Jun-08
Monoclonal Antibody to BAP31 (CC-1)
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SYNONYMS anti-B Cell Receptor-associated Protein BAP31 MAb (CC-1)
anti-BCR-associated Protein BAP31 MAb (CC-1)
anti-p28 BAP31 MAb (CC-1)
anti-CDM Protein MAb (CC-1)
anti-6C6-AG Tumor-associated Antigen MAb (CC-1)
anti-DXS1357E MAb (CC-1)
PRODUCT LINE Immunology
PRODUCT CATEGORY B Cell Receptor [BCR] & Co-Receptors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-812-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Goat
Sheep
Monkey
Others
CLONE: CC-1
ISOTYPE: Rat IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 0.5mg/ml
PURITY: >95% (SDS-PAGE)
FORMULATION: Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Solubilized membranes of the IMR-32 human neuroblastoma cell line.
SPECIFICITY: Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31. Does not recognize mouse and rat BAP31.
APPLICATION: ELISA (capture)
Immunocytochemistry (1:1'000-1:10'000; works on endogenous)
Immunohistochemistry (frozen sections (1:1'000-1:10'000); works on endogenous)
Immunoprecipitation
Western Blot (1:1'000-1:6'000; works on endogenous).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells: H.A. Manley and V.A. Lennon; J. Histochem. Cytochem. 49, 1235 (2001) Abstract; Full Text
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils: K. Zen, et al.; J. Biol. Chem. 279, 44924 (2004) Abstract; Full Text
Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules: J.J. Ladasky, et al.; J. Immunol. 177, 6172 (2006) Abstract
General Information
BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca2+-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol. 20, 6731 (2000) Abstract; Full Text
Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol: D.G. Breckenridge, et al.; J. Cell. Biol. 160, 1115 (2003) Abstract; Full Text
Bap31 Is an Itinerant Protein that Moves between the Peripheral ER and a Juxtanuclear Compartment Related to ER-associated Degradation: Y. Wakana, et al.; Mol. Biol. Cell 19, 1825 (2008) Abstract; Full Text
 
 
ALX-804-224 Revised 25-Apr-08
Monoclonal Antibody to Bax (6A7) (Active Monomer)
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SYNONYMS anti-BCL2L4 MAb (6A7) (Active Monomer)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bax / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-224-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 6A7
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 12-24 of human Bax.
SPECIFICITY: Recognizes the active monomeric form of human, mouse and rat Bax. Does not cross-react with Bax complexed with either Bcl-2 or Bcl-XL or to inactive monomer.
APPLICATION: ELISA (0.5-1µg/ml)
Immunocytochemistry (0.5-1µg/ml)
Immunoprecipitation (in detergent [1, 2])
Western Blot (0.5-1µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening aliquot contents and freeze at -20°C.
Product Specific Literature References
[1] Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis: Y.T. Hsu, et al.; PNAS 94, 3668 (1997) Abstract; Full Text
[2] Nonionic detergents induce dimerization among members of the Bcl-2 family: Y.T. Hsu and R.J. Youle; J. Biol. Chem. 272, 13829 (1997) Abstract; Full Text
[3] Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations: Y.T. Hsu and R.J. Youle; J. Biol. Chem. 273, 1077 (1998) Abstract; Full Text
[4] Direct addition of BimL to mitochondria does not lead to cytochrome c release: O. Terradillos, et al.; FEBS Lett. 522, 29 (2002) Abstract; Full Text
[5] Interaction with a membrane surface triggers a reversible conformational change in Bax normally associated with induction of apoptosis: J.A. Yethon, et al.; J. Biol. Chem. 278, 48935 (2003) Abstract
[6] Vinblastine-induced apoptosis is mediated by discrete alterations in subcellular location, oligomeric structure, and activation status of specific Bcl-2 family members: M. Upreti, et al.; J. Biol. Chem. 281, 15941 (2006) Abstract; Full Text
General Information
Bax and Bak operate in both the endoplasmatic reticulum (ER) and mitochondria as an essential gateway for selected apoptotic signals [1]. A role for Bcl-2 at the ER has been suggested earlier [2] as Bcl-2 may regulate calcium fluxes between the ER and the mitochondria. For a review on Ca2+ and its role in apoptosis see [3].
General Literature References
[1] BAX and BAK regulation of endoplasmic reticulum Ca2+: a control point for apoptosis: L. Scorrano, et al.; Science 300, 135 (2003) Abstract
[2] New insights in the role of Bcl-2 Bcl-2 and the endoplasmic reticulum: J. Rudner, et al.; Apoptosis 7, 441 (2002) Abstract
[3] Calcium signaling and apoptosis: G. Hajnoczky, et al.; Biochem. Biophys. Res. Commun. 304, 445 (2003) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-225 Revised 03-Jan-07
Monoclonal Antibody to Bax (human) (2D2)
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SYNONYMS anti-BCL2L4 (human) MAb (2D2)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bax / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-225-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2D2
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 3-16 of human Bax.
SPECIFICITY: Recognizes human Bax independent of its conformational state.
APPLICATION: ELISA (0.5-1µg/ml)
Immunocytochemistry (0.5-1µg/ml)
Immunoprecipitation (in detergent [1, 2])
Western Blot (0.5-1µg/ml).
Optimal conditions should be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
[1] Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis: Y.T. Hsu, et al.; PNAS 94, 3668 (1997) Abstract; Full Text
[2] Nonionic detergents induce dimerization among members of the Bcl-2 family: Y.T. Hsu and R.J. Youle; J. Biol. Chem. 272, 13829 (1997) Abstract; Full Text
[3] Direct addition of BimL to mitochondria does not lead to cytochrome c release: O. Terradillos, et al.; FEBS Lett. 522, 29 (2002) Abstract; Full Text
[4] Interaction with a membrane surface triggers a reversible conformational change in Bax normally associated with induction of apoptosis: J.A. Yethon, et al.; J. Biol. Chem. 278, 48935 (2003) Abstract
General Information
Bax and Bak operate in both the endoplasmatic reticulum (ER) and mitochondria as an essential gateway for selected apoptotic signals [1]. A role for Bcl-2 at the ER has been suggested earlier [2] as Bcl-2 may regulate calcium fluxes between the ER and the mitochondria. For a review on Ca2+ and its role in apoptosis see [3].
General Literature References
[1] BAX and BAK regulation of endoplasmic reticulum Ca2+: a control point for apoptosis: L. Scorrano, et al.; Science 300, 135 (2003) Abstract
[2] New insights in the role of Bcl-2 Bcl-2 and the endoplasmic reticulum: J. Rudner, et al.; Apoptosis 7, 441 (2002) Abstract
[3] Calcium signaling and apoptosis: G. Hajnoczky, et al.; Biochem. Biophys. Res. Commun. 304, 445 (2003) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-223 Revised 13-Mar-06
Monoclonal Antibody to Bcl-2 (human) (8C8)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-223-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 8C8
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Synthetic peptide corresponding to aa 41-54 (G41AAPAPGIFSSQPPC54) of human Bcl-2.
SPECIFICITY: Recognizes human Bcl-2.
APPLICATION: Western Blot (0.5-1µg/ml).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening aliquot contents and freeze at -20°C.
Product Specific Literature References
Movement of Bax from the cytosol to m