ALEXIS Biochemicals: TRAIL-OPG (human) ELISA Kit

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APO-54N-028

Revised 02-Apr-08

Free Osteoprotegerin (human) Detection Set [For ELISA Application]
SYNONYMS	Free OPG (human) Detection Set [For ELISA Application] Free TNFRSF 11B (human) Detection Set [For ELISA Application] Free Osteoclastogenesis Inhibitory Factor (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-028-KI01		1 Set	540.00 USD

Product Specification

SENSITIVITY:	2pg/ml (range 0 to 250 pg/ml).
QUANTITY:	5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human free osteoprotegerin (not bound to RANKL or TRAIL) in cell culture supernatant, plasma and serum.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

OPG (Osteoprotegerin; also called Osteoclastogenesis Inhibitory Factor or TNFRSF11B) is a secreted member of the TNF (Tumor necrosis factor) receptor superfamily of proteins [1]. OPG, composed of 380 amino acids, is expressed as monomer and secreted as disulphide-homodimeric protein [2]. Produced by numerous cell types and tissues, its expression pattern targets the skeletal, immune, and vascular systems. Osteoblasts secrete OPG that protects the skeleton from excessive bone resorption by acting as a decoy receptor for the ligand nuclear factor-κB ligand (RANKL), preventing it from interacting with its receptor RANK [3]. OPG/RANK/RANKL molecular triumvirat plays a pivotal function in the regulation of the immune system through dendritic cells [4]. OPG-deficient mice exhibit severe osteoporosis and vascular calcification of the aorta and renal arteries [5]. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD), a bone disease [6]. OPG also functions as a soluble receptor for the TNF ligand TRAIL (TNF-related apoptosis-inducing ligand) preventing it to activate apoptotic signaling cascase and therefore providing cancer cells with a survival advantage [7].

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O00300: Osteoprotegerin (human)

General Literature References

[1] Biology of RANK, RANKL, and osteoprotegerin: B.F. Boyce & L. Xing; Arthritis Res. Ther. 9 Suppl 1, S1 (2007) Abstract
[2] Osteoprotegerin: a novel secreted protein involved in the regulation of bone density: W.S. Simonet, et al.; Cell 89, 309 (1997) Abstract
[3] Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation: D.L. Lacey, et al.; Cell 93, 165 (1998) Abstract
[4] RANKL, RANK, osteoprotegerin: key partners of osteoimmunology and vascular diseases: M. Baud huin, et al.; Cell Mol. Life Sci. 64, 2334 (2007) Abstract
[5] Osteoprotegerin reverses osteoporosis by inhibiting endosteal osteoclasts and prevents vascular calcification by blocking a process resembling osteoclastogenesis: H. Min, et al.; J. Exp. Med. 192, 463 (2000) Abstract
[6] Paget’s disease of bone and genetic disorders of RANKL/OPG/RANK/NF-kappaB signaling: M.P. Whyte; Ann. N. Y. Acad. Sci. 1068, 143 (2006) Abstract
[7] Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL: J.G. Emery, et al.; J. Biol. Chem. 273, 14363 (1998) Abstract; Full Text

Further Categories Containing This Product:

ELISA & EIA Kits

APO-54N-042

Revised 04-Dec-08

Free Osteoprotegerin (human) ELISA Kit
SYNONYMS	Free Tumor Necrosis Factor Receptor Superfamily Member 11B (human) ELISA Kit Free Osteoclastogenesis Inhibitory Factor (human) ELISA Kit Free OCIF (human) ELISA Kit Free OPG (human) ELISA Kit
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-042-KI01		1 Kit	780.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	20pg/ml (range 0.031 to 2ng/ml).
QUANTITY:	For 96 wells (~80 tests).
APPLICATION:	For the quantitative determination of free human OPG (not complexed to RANKL or TRAIL) in biological fluids (serum, plasma and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O00300: TR11B (human)
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

ELISA & EIA Kits

ALX-804-813

Revised 26-Oct-07

Monoclonal Antibody to Osteoprotegerin (human) (Bony-1)
SYNONYMS	anti-OPG (human) MAb (Bony-1) anti-TNFRSF 11B (human) MAb (Bony-1) anti-Osteoclastogenesis Inhibitory Factor (human) MAb (Bony-1)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-813-C100		100 µg	320.00 USD
ALX-804-813B-C100	Biotin	100 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Bony-1
ISOTYPE:	Rat IgG2a
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human OPG (osteoprotegerin) cysteine-rich region (aa 22-202) (Prod. No. ALX-522-007).
SPECIFICITY:	Recognizes human OPG.
APPLICATION:	ELISA Flow Cytometry (1:1’000) Immunoprecipitation (1:200)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.

Product Images

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General Information

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Osteoprotegerin: U. Feige; Ann. Rheum. Dis. 60, Suppl 3, iii81 (2001) Abstract; Full Text
Osteoprotegerin is a soluble decoy receptor for tumor necrosis factor-related apoptosis-inducing ligand/Apo2 ligand and can function as a paracrine survival factor for human myeloma cells: C.M. Shipman and P.I. Croucher; Cancer Res. 63, 912 (2003) Abstract; Full Text

Related Products

ALX-522-007	Osteoprotegerin (human):Fc (human) (recombinant)
ALX-210-945	Polyclonal Antibody to Osteoprotegerin (human) (AT121)

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-804-207

Revised 13-Dec-04

Monoclonal Antibody to Osteoprotegerin (human) (NT) (OPG-13)
SYNONYMS	anti-OPG (human) MAb (NT) (OPG-13) anti-TNFRSF 11B (human) MAb (OPG-13) anti-Osteoclastogenesis Inhibitory Factor (human) MAb (NT) (OPG-13)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-207-C100		100 µg	508.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	OPG-13
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml after reconstitution.
FORMULATION:	Lyophilized from sterile filtered solution of protein G affinity purified antibody in 0.05M phosphate buffer, pH 7.2, containing 0.1M sodium chloride.
RECONSTITUTION:	Reconstitute with 100µl of distilled water. Activity is not affected by slight turbidity. Clarify by centrifugation.
IMMUNOGEN:	Synthetic peptide corresponding to N-terminal aa 21-39 of human OPG (osteoprotegerin).
SPECIFICITY:	Recognizes human OPG.
APPLICATION:	ELISA Western Blot (1:10'000).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

General Information

Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 aa with molecular weight of 55kDa as a monomer and 110kDa as a disulphide-linked dimer. It acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-804-532

Revised 15-Apr-08

Monoclonal Antibody to Osteoprotegerin (human) (OPG-01)
SYNONYMS	anti-OPG (human) MAb (OPG-01) anti-TNFRSF 11B (human) MAb (OPG-01) anti-Osteoclastogenesis Inhibitory Factor (human) MAb (OPG-01)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-532-C100		100 µg	508.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	OPG-01
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml after reconstitution.
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Lyophilized from sterile filtered solution in 0.05M phosphate buffer, pH 7.2, containing 0.1M sodium chloride.
RECONSTITUTION:	Reconstitute with 100µl distilled water. Activity is not affected by slight turbidity. Clarify by centrifugation.
IMMUNOGEN:	Recombinant human OPG (osteoprotegerin).
SPECIFICITY:	Recognizes human OPG.
APPLICATION:	ELISA Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

General Information

Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor superfamily. OPG consists of 401 aa with molecular weight of 55kDa as a monomer and 110kDa as a disulfide-linked dimer. It acts as a soluble factor in the regulation of bone mass and may be beneficial in the treatment of osteoporosis associated with increased osteoclast activity.

Further Categories Containing This Product:

Monoclonal Antibodies

ALX-522-007

Revised 30-Jul-07

Osteoprotegerin (human):Fc (human) (recombinant)
SYNONYMS	OPG (human):Fc (human) (recombinant) TNFRSF 11B (human):Fc (human) (recombinant) Osteoclastogenesis Inhibitory Factor (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	OPG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-007-C050		50 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	Produced in HEK 293 cells. The cysteine-rich region of human OPG (osteoprotegerin) (aa 22-202) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human and mouse RANKL and TRAIL.
BIOLOGICAL ACTIVITY:	Inhibits rhsTRAIL-mediated apoptosis at a concentration range of 5-20µg/ml, as well as rhsRANKL-induced osteoclastogenesis and stimulation of dendritic cells. Concentrations of rhOPG:Fc required to inhibit may vary depending on the cell type studied and on the concentration of rhsTRAIL used to kill the cells.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

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Product Specific Literature References

Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
A CD40-CD95L fusion protein interferes with CD40L-induced prosurvival signaling and allows membrane CD40L-restricted activation of CD95: C. Assohou-Luty, et al.; J. Mol. Med. 84, 785 (2006) Abstract

General Information

Osteoprotegerin (OPG) is a secreted tumor necrosis factor receptor homologue that inhibits osteoclastogenesis and increases bone density in vivo. OPG contains 4 cysteine-rich repeats and 2 ‘death domains’ (DD). OPG binds to RANKL (TRANCE) and with a similar affinity to TRAIL.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O00300: Osteoprotegrin (human) (precursor) AfCS Signalling Gateway link A002819: Osteoprotegrin (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.