ALEXIS Biochemicals: CD14 (human) (recombinant)

ALX-201-133

Revised 19-Feb-08

CD14 (human) (recombinant)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	CD14 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-133-C010		10 µg	270.00 USD

Product Specification

MW:	~50kDa.
SOURCE/HOST:	Produced in CHO cells.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	~90-95% (SDS-PAGE)
PURITY DETAIL:	Affinity purified using MAb to CD14 (human) (biG 2/RoMo-1) (Prod. No. ALX-804-498).
FORMULATION:	Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT:	<0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION:	Reconstitute with 10µl distilled water. Further dilutions should be made with PBS
BIOLOGICAL ACTIVITY:	Inhibits binding of LPS to CD14. Up to 20µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6x10⁵ CD14⁺ CHO transfectants (FACS).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Both membrane-bound and soluble forms of CD14 bind to gram-negative bacteria: R.S. Jack, et al.; Eur. J. Immunol. 25, 1436 (1995) Abstract
The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms: F. Stelter, et al.; Eur. J. Biochem. 236, 457 (1996) Abstract
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation: K. Le-Barillec, et al.; J. Clin. Invest. 103, 1039 (1999) Abstract; Full Text

General Information

The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore it is present in a soluble form in mouse serum, urine and other body fluids.

MANUFACTURER

Manufactured by Biometec.