ALEXIS Biochemicals: CD30L, Soluble (human) (recombinant)

ALX-522-028

Revised 24-Mar-06

CD30L, Soluble (human) (recombinant)
SYNONYMS	CD153, Soluble (human) (recombinant) TNFSF 8, Soluble (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CD30 & CD30L / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-028-C010		10 µg	200.00 USD
ALX-522-028-3010	SuperPack	3x10 µg	410.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
MW:	~35kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of recombinant human CD30L (CD153) (aa 63-234) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG^®-tag.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds to human CD30 (Ki-1).
APPLICATION:	Can be used together with an enhancer (Prod. No. 804-034, see Set Prod. No. 850-066) to detect membrane-bound human CD30. rhsCD30L saturates its receptor at a concentration range of 12-4000ng/ml.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Specific Literature References

Selective enrichment of CD30-expressing cells within the blast region of lymphocytes from patients with primary HIV infection (PHI): P. Biswas, et al.; J. Biol. Regul. Homeost. Agents 16, 33 (2002) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract

General Information

The CD30 ligand (CD30L, CD153) is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P32971: CD30L (human)
AfCS Signalling Gateway link A000555: CD30L (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

CD Proteins • Recombinant Proteins / Fusion Proteins

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