ALEXIS Biochemicals: CD40L, Soluble (human) (recombinant) Set

ALX-850-064

Revised 23-Apr-08

CD40L, Soluble (human) (recombinant) Set
SYNONYMS	TNFSF 5, Soluble (human) (recombinant) Set CD154, Soluble (human) (recombinant) Set
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CD40 & CD40L [CD154] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-064-KI01		1 Set	440.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	Produced in E. coli. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG^®-tag.
CONCENTRATION:	CD40L: 0.1mg/ml after reconstitution. Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS:	10µg of CD40L, Soluble (human) (recombinant) (Prod. No. ALX-522-015) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034), which increases the biological activity of rhsCD40L at least 1’000-fold.
PURITY:	≥95% (SDS-PAGE).
FORMULATION:	Lyophilized. Contains PBS.
RECONSTITUTION:	Reconstitute CD40L with 100µl and each vial of enhancer with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY:	CD40L binds to human CD40.
APPLICATION:	Can be used to activate human B cells and dendritic cells. Stimulates growth of human B cells. For stimulation of mouse cells via CD40 use MAb to CD40 (mouse) (FGK45) (Prod. No. ALX-805-046).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Specific Literature References

Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
CD45 inhibits CD40L-induced microglial activation via negative regulation of the Src/p44/42 MAPK pathway: J. Tan, et al.; J. Biol. Chem. 275, 37224 (2000) Abstract; Full Text
CD40 induces apoptosis in carcinoma cells through activation of cytotoxic ligands of the tumor necrosis factor superfamily: A.G. Eliopoulos, et al.; Mol. Cell. Biol. 20, 5503 (2000) Abstract
Brain dendritic cells and macrophages/microglia in central nervous system inflammation: H.G. Fischer & G. Reichmann; J. Immunol. 166, 2717 (2001) Abstract; Full Text
Synergy between CD40 ligation and IL-4 on fibroblast proliferation involves IL-4 receptor signaling: S.P. Atamas, et al.; J. Immunol. 168, 1139 (2002) Abstract; Full Text
The novel IgD binding protein from Moraxella catarrhalis induces human B lymphocyte activation and Ig secretion in the presence of Th2 cytokines: A. Gjorloff Wingren, et al.; J. Immunol. 168, 5582 (2002) Abstract; Full Text
Platelet-Activating Factor Mediates CD40-Dependent Angiogenesis and Endothelial-Smooth Muscle Cell Interaction: S. Russo, et al.; J. Immunol. 171, 5489 (2003) Abstract
Induction of antigen-specific CTL by recombinant HIV trans-activating fusion protein-pulsed human monocyte-derived dendritic cells: Y. Tanaka, et al.; J. Immunol. 170, 1291 (2003) Abstract; Full Text
IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo: A. Soruri, et al.; J. Immunol. 170, 3306 (2003) Abstract; Full Text
CD40 induces human multiple myeloma cell migration via phosphatidylinositol 3-kinase/AKT/NF-kappa B signaling: Y.T. Tai, et al.; Blood 101, 2762 (2003) Abstract
TNF receptor-associated factor 6 is an essential mediator of CD40-activated proinflammatory pathways in monocytes and macrophages: L. Mukundan, et al.; J. Immunol. 174, 1018 (2005) Abstract; Full Text
Enhanced activation of human dendritic cells by inducible CD40 and Toll-like receptor-4 ligation: N. Lapteva, et al.; Cancer Res. 67, 10528 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

CD40L (Prod. No. ALX-522-015) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions.

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

CD Kits & Sets • Receptor / Ligand + Enhancer Set

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