ALEXIS Biochemicals: CD40L, Soluble (mouse) (recombinant) Set

ALX-850-075

Revised 23-Apr-08

CD40L, Soluble (mouse) (recombinant) Set
SYNONYMS	CD154, Soluble (mouse) (recombinant) Set TNFSF 5, Soluble (mouse) (recombinant) Set gp39, Soluble (mouse) (recombinant) Set
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CD40 & CD40L [CD154] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-075-KI01		1 Set	495.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG^®-tag.
CONCENTRATION:	CD40L: 0.1mg/ml after reconstitution. Enhancer: 1mg/ml after reconstitution.
KIT/SET CONTAINS:	10µg of CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070) and 2x50µg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity of CD40L at least 1’000-fold.
PURITY:	≥90% (SDS-PAGE).
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute CD40L with 100µl sterile water and each vial of enhancer with 50µl of sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY:	CD40L binds to human and mouse CD40 in an ELISA assay.
APPLICATION:	ELISA Functional Application: Stimulates the proliferation of mouse B cells and dendritic cells. The activity of CD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of the cross-linking enhancer (Prod. No. ALX-804-034).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

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Product Specific Literature References

The persistent elimination of B cells responding to blood group A carbohydrates by synthetic group A carbohydrates and B-1 cell differentiation blockade: novel concept in preventing antibody-mediated rejection in ABO-incompatible transplantation: T. Irei, et al.; Blood 110, 4567 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION	CD40L (Prod. No. ALX-522-070) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10µg/ml of CD40L together with 1-2µg/ml of enhancer. This may vary by cell type and experimental conditions. FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER	Manufactured by Apotech Corporation.