ALEXIS Biochemicals: CFSE

ALX-610-030

Revised 23-Aug-06

CFSE
SYNONYMS	5(6)-(N-Succinimidyloxycarbonyl)-3',6',0,0'-diacetylfluorescein 5(6)-Carboxyfluorescein diacetate, N-succinimidyl ester-
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Dyes / Stains / Fluorescent Probes / Fluorescent Labels

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-610-030-M025		25 mg	190.00 USD

Product Specification

FORMULA:	C₂₉H₁₉NO₁₁
MW:	557.5
CAS NUMBER:	150347-59-4
PURITY:	≥95%
APPEARANCE:	White or slightly yellow powder.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide or methanol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Non-toxic cell permeable fluorescent dye that reacts with amine compounds. Readily accumulates inside viable cells. Hardly leaks from cells when covalently attached to intracellular proteins. Hydrolyzed CFSE emits fluorescence (Ex(max): 495nm; Em(max): 519nm). Therefore, CFSE is utilized for the long-term observation of cell activities by fluorescent microscopy as well as for viable cell detection.

Product Specific Literature References

New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy: S.A. Weston & C.R. Parish; J. Immunol. Methods 133, 87 (1990) Abstract
Use of fluorescent dyes in the determination of adherence of human leucocytes to endothelial cells and the effect of fluorochromes on cellular function: L.S. De Clerck, et al.; J. Immunol. Methods 172, 115 (1994) Abstract
A simple method for evaluating the rejection of grafted spleen cells by flow cytometry and tracing adoptively transferred cells by light microscopy: S. Oehen, et al.; J Immunol Methods 207, 33 (1997) Abstract
Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester: A.B. Lyons; Immunol. Cell Biol. 77, 509 (1998) Abstract
The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo: J. Mintern, et al.; Immunol. Cell Biol. 77, 539 (1999) Abstract
Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven: B.F. de St Groth, et al.; Immunol. Cell Biol. 77, 530 (1999) Abstract
Optimization of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester for labeling human intervertebral disc cells in vitro: H.E. Gruber, et al.; Biotech. Histochem. 75, 118 (2000) Abstract
Flow cytometric cell division tracking using nuclei: J. Hasbold & P.D. Hodgkin; Cytometry 40, 230 (2000) Abstract
Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution: A.B. Lyons; J. Immunol. Methods 243, 147 (2000) Abstract
Flow cytometry analysis of the effect of allopurinol and the dinitroaniline compound (Chloralin) on the viability and proliferation of Leishmania infantum promastigotes: S.W. Kamau, et al.; BMC Pharmacol. 1, 1 (2001) Abstract
Quantifying the frequency of alloreactive T cells in vivo: new answers to an old question: E.J. Suchin, et al.; J. Immunol. 166, 973 (2001) Abstract

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