ALEXIS Biochemicals: CPF4

ALX-620-079

Revised 11-Aug-06

CPF4
SYNONYMS	Coumarin phosphodiester-linked fluorescein
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Phosphodiesterases/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-079-C250		250 µg	381.00 USD
ALX-620-079-C500		500 µg	667.00 USD

Product Specification

FORMULA:	C₄₄H₂₈ClN₂O₁₄P
MW:	875.1
PURITY:	≥99% (¹H-NMR)
APPEARANCE:	Yellow solid.
SOLUBILITY:	Soluble in DMSO (10mM) or in aqueous buffers, pH>8.5.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from light. Keep cool and dry.

Product Images

Please click on thumbnails to enlarge.

Product Description

Enzyme-cleavable FRET sensor molecule for the ratiometric measurement of phosphodiesterase activity. This is an effective method for the monitoring of phosphodiesterase activity in real time with high sensitivity; a large spectral shift in the emission spectrum is noticed after hydrolysis of the phosphodiester linker by phosphodiesterase I (Ex(max): 370nm; Em(max) uncleaved: 515nm; Em(max) cleaved: 450nm). The role of NPPs (pyrophosphatases/phosphodiesterases) can also be investigated.

Product Specific Literature References

Design and synthesis of an enzyme-cleavable sensor molecule for phosphodiesterase activity based on fluorescence resonance energy transfer: H. Takakusa, et al.; J. Am. Chem. Soc. 124, 1653 (2002) Abstract
Design and Synthesis of Intramolecular Resonance-Energy Transfer Probes for Use in Ratiometric Measurements in Aqueous Solution: Y. Kawanishi, et al.; Angew. Chem. Int. Ed. Engl. 39, 3438 (2000) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

FRET (fluorescence resonance energy transfer) is a radiationless transmission of an energy quantum between two fluorophores which are in close proximity. Cleavage of a chemical bond between these fluorophores, which have an overlap in their spectra, results in a large shift in the emission spectra. Addition of phosphodiesterase I, which catalyzes the hydrolysis of phosphodiester bonds (substrates are nucleic acids and cyclic nucleotides), to an aqueous solution of CPF4 results in an increase in the donor fluorescence and a decrease in the acceptor fluorescence. CPF4 is an effective tool for the monitoring of the activity of phosphodiesterases in real time with high sensitivity.

Further Categories Containing This Product:

Dyes/Stains/Fluorescent Probes/Fluorescent Labels

another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.