ALEXIS Biochemicals: 4α-Phorbol 12,13-didecanoate

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ALX-340-059

Revised 10-Jul-08

5’,6’-Epoxyeicosatrienoic acid
SYNONYMS	(±)-5(6)-Epoxy-8Z,11Z,14Z-eicosatrienoic acid 5',6'-EET
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	TRPV2, TRPV3, TRPV4 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-340-059-C025		25 µg	60.00 USD
ALX-340-059-C050		50 µg	90.00 USD

Product Specification

FORMULA:	C₂₀H₃₂O₃
MW:	320.5
CONCENTRATION:	0.1mg/ml
PURITY:	≥98%
FORMULATION:	Liquid. Solution in acetonitrile.
SOLUBILITY:	50mg/ml soluble in 100% ethanol, DMSO or dimethyl formamide; slightly soluble in PBS, pH 7.2 (1mg/ml).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -80°C. Keep aqueous solutions on ice and use within 12 hours.

Product Description

Endogenous TRPV4 agonist (K_i=150nM).

Product Specific Literature References

Anandamide and arachidonic acid use epoxyeicosatrienoic acids to activate TRPV4 channels: H. Watanabe, et al.; Nature 424, 434 (2003) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

To change the solvent, evaporate the acetonitrile under a gentle stream of nitrogen and immediately add the solvent of choice.

ALX-400-045

Revised 31-Aug-07

2-Aminoethoxydiphenyl borate
SYNONYMS	2-APB
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Ca2+ Modulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-400-045-M100		100 mg	30.00 USD

Product Specification

FORMULA:	C₁₄H₁₆BNO
MW:	225.1
CAS NUMBER:	524-95-8
RTECS:	ED6150000
PURITY:	≥98%
APPEARANCE:	White crystalline solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide or 100% ethanol; also soluble in PBS, pH 7.2.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C. Stock solutions are stable for 2 months at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Hygroscopic. Packaged under inert gas.

Product Description

Cell permeable modulator of Ins(1,4,5)-P₃-induced Ca²⁺ release. A prototype drug for a group of structurally related calcium channel blockers in human platelets. Has been shown, in the absence of other stimuli, to activate TRPV1, V2 and V3, but not TRPV4, V5 and V6 expressed in HEK 293 cells.

Product Specific Literature References

2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release: T. Maruyama, et al.; J. Biochem. 122, 498 (1997) Abstract
Inhibition of SERCA Ca2+ pumps by 2-aminoethoxydiphenyl borate (2-APB). 2-APB reduces both Ca2+ binding and phosphoryl transfer from ATP, by interfering with the pathway leading to the Ca2+-binding sites: J.G. Bilmen, et al.; Eur. J. Biochem. 269, 3678 (2002) Abstract
2-aminoethoxydiphenyl borate (2-APB) is a reliable blocker of store-operated Ca2+ entry but an inconsistent inhibitor of InsP3-induced Ca2+ release: M.D. Bootman, et al.; FASEB J. 16, 1145 (2002) Abstract
Activation of TRPV4 channels (hVRL-2/mTRP12) by phorbol derivatives: H. Watanabe, et al.; J. Biol. Chem. 277, 13569 (2002) Abstract
2-Aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2 and TRPV3: H.Z. Hu, et al.; J. Biol. Chem. 279, 35741 (2004) Abstract; Full Text
TRPV4 calcium entry channel: a paradigm for gating diversity: B. Nilius, et al.; Am. J. Physiol. Cell Physiol. 286, C195 (2004) Abstract
2APB- and JTV519(K201)-sensitive micro Ca2+ waves in arrhythmogenic Purkinje cells that survive in infarcted canine heart: P.A. Boyden, et al.; Heart Rhythm 1, 218 (2004) Abstract
Inhibition of glutamate-induced delayed calcium deregulation by 2-APB and La3+ in cultured cortical neurones: C. Chinopoulos, et al.; J. Neurochem. 91, 471 (2004) Abstract
2-aminoethoxydiphenyl borate activates and sensitizes the heat-gated ion channel TRPV3: M.K. Chung, et al.; J. Neurosci. 24, 5177 (2004) Abstract; Full Text
The blocking of capacitative calcium entry by 2-aminoethyl diphenylborate (2-APB) and carboxyamidotriazole (CAI) inhibits proliferation in Hep G2 and Huh-7 human hepatoma cells: A. Enfissi, et al.; Cell Calcium 36, 459 (2004) Abstract
Induction of cholestasis in the perfused rat liver by 2-aminoethyl diphenylborate, an inhibitor of the hepatocyte plasma membrane Ca2+ channels: R.B. Gregory, et al.; J. Gastroenterol. Hepatol. 19, 1128 (2004) Abstract
2-aminoethoxydiphenyl borate inhibits agonist-induced Ca2+ signals by blocking inositol trisphosphate formation in acutely dissociated mouse pancreatic acinar cells: J. Wu, et al.; Pflugers Arch. 448, 592 (2004) Abstract
2-aminoethoxydiphenyl borate stimulates pulmonary C neurons via the activation of TRPV channels: Q. Gu, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 288, L392 (2005) Abstract
2-Aminoethoxydiphenyl borate perturbs hormone-sensitive calcium stores and blocks store-operated calcium influx pathways independent of cytoskeletal disruption in human A549 lung cancer cells: S. Padar, et al.; Biochem. Pharmacol. 69, 1177 (2005) Abstract
Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltage-dependent effect: S.Z. Xu, et al.; Br. J. Pharmacol. 145, 405 (2005) Abstract; Full Text
2-Aminoethoxydiphenyl borate (2-APB) stimulates a conformationally coupled calcium release pathway in the NG115-401L neuronal cell line: D.D. Bose & D.W. Thomas; Neuropharmacology 50, 532 (2006) Abstract
2-aminoethoxydiphenyl borate as a prototype drug for a group of structurally related calcium channel blockers in human platelets: Y. Dobrydneva, et al.; Mol. Pharmacol. 69, 247 (2006) Abstract

Further Categories Containing This Product:

TRPV2, TRPV3, TRPV4 / Related Products • TRP Channels Other Products • TRPV1 Agonists and Antagonists / Related Products

ALX-445-006

Revised 03-Dec-07

4α-Phorbol 12,13-didecanoate
SYNONYMS	4α-PDD
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Phorbols / Phorbol Esters

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-445-006-M001		1 mg	35.00 USD
ALX-445-006-M005		5 mg	110.00 USD

Product Specification

FORMULA:	C₄₀H₆₄O₈
MW:	672.9
CAS NUMBER:	27536-56-7
PURITY:	≥98% (HPLC)
APPEARANCE:	Lyophilized.
SOLUBILITY:	Soluble in DMSO, methanol, 100% ethanol, acetone, ether or dimethyl formamide.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solution cannot be made in aqueous media. Compound must be dissolved in an organic solvent prior to dilution into aqueous media. Add calculated volume of organic solvent to vial to prepare concentrated stock solution. Intermediate dilutions into the same organic solvent are recommended prior to final dilution into aqueous media.
HANDLING:	Protect from light.
HAZARD:	HIGHLY IRRITANT. VERY TOXIC. MAY BE CARCINOGENIC.

Product Description

Activator of TRPV4. Negative control for phorbol 12,13-didecanoate (PDD) (Prod. No. ALX-445-002).

Product Specific Literature References

TRPV4 calcium entry channel: a paradigm for gating diversity: B. Nilius, et al.; Am. J. Physiol. Cell Physiol. 286, C195 (2004) Abstract
Activation of TRPV4 channels (hVRL-2/mTRP12) by phorbol derivatives: H. Watanabe, et al.; J. Biol. Chem. 277, 13569 (2002) Abstract