ALEXIS Biochemicals: UNC5A (human):Fc (human) (recombinant)

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Androgens & Androgen Receptors/Related Products

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Dependence Receptors Other Products

ALX-270-476

Revised 11-Mar-08

Bicalutamide
SYNONYMS	N-(4-Cyano-3-(trifluoromethyl)phenyl)-3-((4-fluorophenyl)sulfonyl)-2-hydroxy-2-methylpropanamide
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Androgens & Androgen Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-476-M100		100 mg	40.00 USD
ALX-270-476-M500		500 mg	140.00 USD
ALX-270-476-G001		1 g	220.00 USD

Product Specification

FORMULA:	C₁₈H₁₄F₄N₂O₄S
MW:	430.4
CAS NUMBER:	90357-06-5
SOURCE/HOST:	Synthetic.
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in acetone and tetrahydrofuran; slightly soluble in 100% ethanol or methanol; practically insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Nonsteroidal antiandrogen. Effective in the treatment of prostate cancer. Induces cell death by a pathway that is independent of changes in mitochondrial membrane potential and Bcl-2 action.

Product Specific Literature References

Antiproliferative activity of casodex (ICI 176.334) in hormone-dependent tumours: A. Maucher & E. von Angerer; J. Cancer Res. Clin. Oncol. 119, 669 (1993) Abstract
Antiandrogen-induced cell death in LNCaP human prostate cancer cells: E.C. Lee, et al.; Cell Death Differ. 10, 761 (2003) Abstract
Proanthocyanidins from grape seeds inhibit expression of matrix metalloproteinases in human prostate carcinoma cells, which is associated with the inhibition of activation of MAPK and NF kappa B: P.K. Vayalil, et al.; Carcinogenesis 25, 987 (2004) Abstract; Full Text
Wnt3a growth factor induces androgen receptor-mediated transcription and enhances cell growth in human prostate cancer cells: M. Verras, et al.; Cancer Res. 64, 8860 (2004) Abstract; Full Text
Glycogen synthase kinase-3beta activity is required for androgen-stimulated gene expression in prostate cancer: X. Liao, et al.; Endocrinology 145, 2941 (2004) Abstract; Full Text
A phase I pharmacokinetic and pharmacodynamic study of OGX-011, a 2’-methoxyethyl antisense oligonucleotide to clusterin, in patients with localized prostate cancer: K.N. Chi, et al.; J. Natl. Cancer Inst. 97, 1287 (2005) Abstract; Full Text
The mechanisms and managements of hormone-therapy resistance in breast and prostate cancers: K.M. Rau, et al.; Endocr. Relat. Cancer 12, 511 (2005), Review Abstract; Full Text
Signal transduction pathways in androgen-dependent and -independent prostate cancer cell proliferation: P.M. Ghosh, et al.; Endocr. Relat. Cancer 12, 119 (2005) Abstract; Full Text
Recent advances on multiple tumorigenic cascades involved in prostatic cancer progression and targeting therapies: M. Mimeault & S.K. Batra; Carcinogenesis 27, 1 (2006), Review Abstract; Full Text
Bcl-xL is overexpressed in hormone-resistant prostate cancer and promotes survival of LNCaP cells via interaction with proapoptotic Bak: C. Castilla, et al.; Endocrinology 147, 4960 (2006) Abstract; Full Text
Androgen receptor corepressors and prostate cancer: C.J. Burd, et al.; Endocr. Relat. Cancer 13, 979 (2006), Review Abstract; Full Text
Par-4-dependent apoptosis by the dietary compound withaferin A in prostate cancer cells: S. Srinivasan, et al.; Cancer Res. 67, 246 (2006) Abstract; Full Text
Androgen receptor structural and functional elements: role and regulation in prostate cancer: S.M. Dehm & D.J. Tindall; Mol. Endocrinol. 21, 2855 (2007), Review Abstract
Phosphoinositide 3-kinase-independent non-genomic signals transit from the androgen receptor to Akt1 in membrane raft microdomains: B. Cinar, et al.; JBC 282, 29584 (2007) Abstract; Full Text
A novel class of pyranocoumarin anti-androgen receptor signaling compounds: J. Guo, et al.; Mol. Cancer Ther. 6, 907 (2007) Abstract; Full Text
Increased resistance to trail-induced apoptosis in prostate cancer cells selected in the presence of bicalutamide: M. Mitterberger, et al.; Prostate 67, 1194 (2007) Abstract
Interplay of nuclear factor-kappaB and B-myb in the negative regulation of androgen receptor expression by tumor necrosis factor alpha: S. Ko, et al.; Mol. Endocrinol. 22, 273 (2008) Abstract

Further Categories Containing This Product:

Antitumor Agents (Hormone-related)

ALX-430-159

Revised 19-Aug-08

Cyclopamine
SYNONYMS	11-Deoxyjervine
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Hedgehog [Hh] Signalling Pathway

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-159-M001		1 mg	80.00 USD
ALX-430-159-M005		5 mg	320.00 USD

Product Specification

FORMULA:	C₂₇H₄₁NO₂
MW:	411.6
CAS NUMBER:	4449-51-8
MERCK INDEX:	14: 10201
RTECS:	GY0750000
PURITY:	≥98% (TLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in 100% ethanol (20mg/ml), DMF (10mg/ml), methanol (7mg/ml) or DMSO (4mg/ml). Heating to 50-60°C may be necessary. Insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.
HAZARD:	MAY BE TERATOGENIC. HARMFUL.

Product Description

Steroidal alkaloid. Blocks activation of the Hedgehog (Hh) response pathway. Binds to the heptahelical protein bundle of Smoothened. Induces expression of the transcription factor Pdx-1. Disrupts cholesterol biosynthesis. Displays anti-tumor properties. Induces apoptosis. Causes cyclopia.

Product Specific Literature References

The teratogenic Veratrum alkaloid cyclopamine inhibits sonic hedgehog signal transduction: J.P. Incardona, et al.; Development 125, 3553 (1998) Abstract; Full Text
Pancreas development is promoted by cyclopamine, a hedgehog signaling inhibitor: S.K. Kim & D.A. Melton; PNAS 95, 13036 (1998) Abstract; Full Text
Teratogen-mediated inhibition of target tissue response to Shh signaling: M.K. Cooper, et al.; Science 280, 1603 (1998) Abstract
Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine: J. Taipale, et al.; Nature 406, 1005 (2000) Abstract
Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport: J.P. Incardona, et al.; Dev. Biol. 224, 440 (2000) Abstract
Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened: J.K. Chen, et al.; Genes Dev. 16, 2743 (2002) Abstract; Full Text
Small molecule modulation of Smoothened activity: J.K. Chen, et al.; PNAS 99, 14071 (2002) Abstract; Full Text
Medulloblastoma growth inhibition by hedgehog pathway blockade: D.M. Berman, et al.; Science 297, 1559 (2002) Abstract
Hedgehog signalling within airway epithelial progenitors and in small-cell lung cancer: D.N. Watkins, et al.; Nature 422, 313 (2003) Abstract
Widespread requirement for Hedgehog ligand stimulation in growth of digestive tract tumours: D.M. Berman, et al.; Nature 425, 846 (2003) Abstract
Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis: S.P. Thayer, et al.; Nature 425, 851 (2003) Abstract
Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment: D. Qualtrough, et al.; Int. J. Cancer 110, 831 (2004) Abstract
Induction of the differentiation and apoptosis of tumor cells in vivo with efficiency and selectivity: S. Tabs & O. Avci; Eur. J. Dermatol. 14, 96 (2004) Abstract; Full Text
In vitro directed differentiation of mouse embryonic stem cells into insulin-producing cells: T. Leon-Quinto, et al.; Diabetologia 47, 1442 (2004) Abstract
Transforming growth factor (TGF)beta, fibroblast growth factor (FGF) and retinoid signalling pathways promote pancreatic exocrine gene expression in mouse embryonic stem cells: A. Skoudy, et al.; Biochem. J. 379, 749 (2004) Abstract; Full Text
Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway: X. Wu, et al.; Chem. Biol. 11, 1229 (2004) Abstract

Related Products

ALX-350-377	Tomatidine . hydrochloride
ALX-350-378	Jervine

ALX-522-109

Revised 14-Apr-08

DCC (Fibronectin Domain 5) (human) (recombinant)
SYNONYMS	Deleted in Colorectal Cancer Protein (Fibronectin Domain 5) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Netrins & Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-109-C050		50 µg	360.00 USD

Product Specification

SOURCE/HOST:	Produced in E. coli. The fibronectin domain 5 (FBN5) of human DCC (aa 841-941) is fused to a N-terminal tag.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human netrin-1.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Specific Literature References

Netrin-1 expression confers a selective advantage for tumor cell survival in metastatic breast cancer: J. Fitamant, et al.; PNAS 105, 4850 (2008) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q16218: Deleted in Colorectal Cancer Protein (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

Netrin binds discrete subdomains of DCC and UNC5 and mediates interactions between DCC and heparin: B.V. Geisbrecht, et al.; J. Biol. Chem. 278, 32561 (2003) Abstract

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins

ALX-385-032

Revised 16-Jun-08

(R,S)-Equol
SYNONYMS	(±)-Equol 3,4-Dihydro-3-(4-hydroxyphenyl)-2H-1-benzopyran-7-ol 4’,7-Dihydroxyisoflavane
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Flavonoids/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-385-032-M005		5 mg	30.00 USD
ALX-385-032-M025		25 mg	120.00 USD

Product Specification

FORMULA:	C₁₅H₁₄O₃
MW:	242.3
CAS NUMBER:	531-95-3, 94105-90-5
MERCK INDEX:	14: 3644
PURITY:	≥98% (HPLC)
APPEARANCE:	White to light yellow powder.
SOLUBILITY:	Soluble in DMSO, methanol or 100% ethanol; insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Flavonoid. Racemic mixture. Urinary metabolite of daidzein. Inhibits 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced neoplastic cell transformation by targeting the MEK/ERK/p90RSK/activator protein-1 signalling pathway. Shows positive effects on the incidence of prostate cancer and physiological changes after menopause. Functions as a DHT blocker. Preferentially activates estrogen receptor β (ERβ).

Product Specific Literature References

Comparisons of percent equol producers between prostate cancer patients and controls: case-controlled studies of isoflavones in Japanese, Korean and American residents: H. Akaza, et al.; Jpn. J. Clin. Oncol. 34, 86 (2004) Abstract; Full Text
Mammographic density in relation to daidzein-metabolizing phenotypes in overweight, postmenopausal women: C.L. Frankenfeld, et al.; Cancer Epidemiol. Biomarkers Prev. 13, 1156 (2004) Abstract; Full Text
Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback: T.D. Lund, et al.; Biol. Reprod. 70, 1188 (2004) Abstract; Full Text
Phytoestrogens and their human metabolites show distinct agonistic and antagonistic properties on estrogen receptor alpha (ERalpha) and ERbeta in human cells: S.O. Mueller, et al.; Toxicol. Sci. 80, 14 (2004) Abstract; Full Text
High concordance of daidzein-metabolizing phenotypes in individuals measured 1 to 3 years apart: C.L. Frankenfeld, et al.; Br. J. Nutr. 94, 873 (2005) Abstract
Equol, a metabolite of the soybean isoflavone daidzein, inhibits neoplastic cell transformation by targeting the MEK/ERK/p90RSK/activator protein-1 pathway: N.J. Kang, et al.; J. Biol. Chem. 282, 32856 (2007) Abstract; Full Text
Isoflavones, equol and cardiovascular disease: pharmacological and therapeutic insights: K.A. Jackman, et al.; Curr. Med. Chem. 14, 2824 (2007) Abstract

Further Categories Containing This Product:

Estrogen & Estrogen Receptors/Related Products • Antitumor Agents (Hormone-related) • Androgens & Androgen Receptors/Related Products • ERK1 & ERK2/Related Products

ALX-270-482

Revised 23-Apr-08

GANT61
SYNONYMS	NSC 136476 2,2’-[[Dihydro-2-(4-pyridinyl)-1,3(2H,4H)-pyrimidinediyl]bis(methylene)]bis[N,N-dimethyl]-benzenamine
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Hedgehog [Hh] Signalling Pathway

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-482-M001		1 mg	190.00 USD

Product Specification

FORMULA:	C₂₇H₃₅N₅
MW:	429.6
CAS NUMBER:	500579-04-4
PURITY:	≥95%
APPEARANCE:	White to off-white waxy solid.
SOLUBILITY:	Soluble in DMSO (5mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Hygroscopic. Protect from light.
IDENTITY:	Identity determined by ¹H-NMR, ¹³C-NMR and MS.

Product Description

Inhibitor of Hedgehog (Hh) signalling pathway. Blocks the GLI function which constitutes the final step in the Hh pathway. Interferes with GLI1 DNA binding in living cells. Inhibits cell proliferation of tumor cells in vitro in a GLI-dependent manner. Blocks cell growth in an in vivo xenograft model using human prostate cancer cells.

Product Specific Literature References

Inhibition of GLI-mediated transcription and tumor cell growth by small-molecule antagonists: M. Lauth, et al.; PNAS 104, 8455 (2007) Abstract; Full Text

Further Categories Containing This Product:

Antitumor Reagents Other Products

ALX-350-378

Revised 19-Aug-08

Jervine
SYNONYMS	(3β,23β)-17,23-Epoxy-3-hydroxyveratraman-11-one 11-Ketocyclopamine
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Hedgehog [Hh] Signalling Pathway

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-378-M001		1 mg	90.00 USD
ALX-350-378-M005		5 mg	360.00 USD

Product Specification

FORMULA:	C₂₇H₃₉NO₃
MW:	425.6
CAS NUMBER:	469-59-0
MERCK INDEX:	14: 5264
RTECS:	WG9700000
PURITY:	≥98% (TLC)
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO (5mg/ml), 100% ethanol (10mg/ml) or dimethyl formamide (6mg/ml). Slight warming may be required.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for up to 3 months when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C.
HAZARD:	HARMFUL. MAY BE TERATOGENIC.

Product Description

Cell permeable steroidal alkaloid. Structurally similar to cyclopamine (Prod. No. ALX-430-159). Inhibits the sonic hedgehog pathway (shh) by interacting with smoothened. Tomatidine (Prod. No. ALX-430-377) is a useful negative control.

Product Specific Literature References

Teratogen-mediated inhibition of target tissue response to Shh signaling: M.K. Cooper, et al.; Science 280, 1603 (1998) Abstract
Cyclopamine inhibition of Sonic hedgehog signal transduction is not mediated through effects on cholesterol transport: J.P. Incardona, et al.; Dev. Biol. 224, 440 (2000) Abstract
Prx1 and Prx2 are upstream regulators of sonic hedgehog and control cell proliferation during mandibular arch morphogenesis: D. ten Berge, et al.; Development 128, 2929 (2001) Abstract; Full Text
Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium: C.M. Mistretta, et al.; Dev. Biol. 254, 1 (2003) Abstract
Identification of a small molecule inhibitor of the hedgehog signaling pathway: effects on basal cell carcinoma-like lesions: J.A. Williams, et al.; PNAS 100, 4616 (2003) Abstract; Full Text
A defective response to Hedgehog signaling in disorders of cholesterol biosynthesis: M.K. Cooper, et al.; Nat. Genet. 33, 508 (2003) Abstract

SPECIES CROSSREACTIVITY:	Human Mouse Rat Monkey
CLONE:	AN1-15
ISOTYPE:	Rat IgG
FORMULATION:	Lyophilized from purified antibody in PBS.
RECONSTITUTION:	Reconstitute with PBS.
IMMUNOGEN:	241 aa (331-572) fusion protein derived from human androgen receptor.
SPECIFICITY:	Recognizes human, mouse, rat and monkey androgen receptor. Detects a band of ~110kDa by Western blot.
APPLICATION:	Immunocytochemistry (5µg/ml) Immunohistochemistry (frozen sections, paraffin sections (4µg/ml)) Immunoprecipitation Western Blot (1µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-853-C100		100 µg	340.00 USD
ALX-804-853PF-C100	Preservative Free	100 µg	340.00 USD
ALX-804-853TD-T100	ATTO 488	100 tests	380.00 USD
ALX-804-853TS-T100	ATTO 647N	100 tests	380.00 USD

SPECIES CROSSREACTIVITY:	Human
CLONE:	Axel-1
ISOTYPE:	Mouse IgG2aλ
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	ALX-804-853: Liquid. In PBS containing 0.02% sodium azide. ALX-804-853PF: Liquid. In PBS. ALX-804-853TD: Liquid. In PBS containing 0.02% sodium azide. ALX-804-853TS: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human DCC fibronectin domain 5 (Prod. No. ALX-522-109).
SPECIFICITY:	Recognizes human DCC.
APPLICATION:	Flow Cytometry Functional Application: Blocking
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.