ALEXIS Biochemicals: Monoclonal Antibody to Doxorubicin (MAD11)

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ALX-380-242

Revised 03-Apr-08

Chaetocin (high purity)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-242-M001		1 mg	330.00 USD

Product Specification

FORMULA:	C₃₀H₂₈N₆O₆S₄
MW:	696.9
CAS NUMBER:	28097-03-2
RTECS:	FM3032000
SOURCE/HOST:	Isolated from Chaetomium sp. MST-FP2085.
PURITY:	≥99% (HPLC)
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO, dimethyl formamide, methanol or 100% ethanol; poorly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	HARMFUL.

Product Description

Antitumor antibiotic. Specific inhibitor of the lysine-specific methyltransferase SU(VAR)3-9 both in vitro and in vivo.

Product Specific Literature References

Isolation and configuration of Chaetocin: D. Hauser, et al.; Helv. Chim. Acta. 53, 1061 (1970)
Identification of a specific inhibitor of the histone methyltransferase SU(VAR)3-9: D. Greiner, et al.; Nat. Chem. Biol. 1, 143 (2005) Abstract
Chaetocin: a promising new antimyeloma agent with in vitro and in vivo activity mediated via imposition of oxidative stress: C.R. Isham, et al.; Blood 109, 2579 (2007) Abstract

Further Categories Containing This Product:

DNA & RNA Methyl & Alkyl Transferases / Related Products

ALX-380-243

Revised 08-Apr-08

Siomycin
SYNONYMS	Mutabilycin Sporangiomycin Antibiotic 6741-21 Mutabillicin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-243-MC05		0.5 mg	390.00 USD

Product Specification

FORMULA:	C₇₁H₈₁N₁₉O₁₈S₅
MW:	1648.9
CAS NUMBER:	12656-09-6
RTECS:	VX0600000
SOURCE/HOST:	Isolated from Streptomyces sp. MST-AS4617.
PURITY:	≥95% (HPLC)
APPEARANCE:	White solid.
SOLUBILITY:	Soluble in DMSO or dimethyl formamide; partially soluble in methanol or 100% ethanol; poorly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Macrocyclic thiazole antibiotic with potent and selective antibacterial activity. Potent inhibitor of the oncogenic transcription factor forkhead box M1 (FoxM1) that downregulates protein and mRNA levels of FoxM1. Inhibits FoxM1 induced cell growth.

Product Specific Literature References

Studies on siomycin. I. Physicochemical properties of siomycins A, B and C: M. Ebata, et al.; J. Antibiot. 22, 364 (1969)
Identification of a chemical inhibitor of the oncogenic transcription factor forkhead box M1: S.K. Radhakrishnan, et al.; Cancer Res. 22, 364 (2006) Abstract; Full Text
Future roles for FoxM1 inhibitors in cancer treatments: G.R. Adami & H. Ye; Future Oncol. 3, 1 (2007) Abstract; Full Text

Further Categories Containing This Product:

Forkhead Box [FOX] Proteins / Related Products • Other Natural Products - DNA Regulation / Transcription

ALX-380-260

Revised 07-Apr-08

Idarubicin . hydrochloride
SYNONYMS	Idamycin DMDR 4-Demethoxydaunorubicin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics - Apoptosis Inducers & Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-260-M001		1 mg	45.00 USD
ALX-380-260-M005		5 mg	180.00 USD

Product Specification

FORMULA:	C₂₆H₂₇NO₉ . HCl
MW:	497.5 . 36.5
CAS NUMBER:	57852-57-0
MERCK INDEX:	14: 4886
RTECS:	HB7877000
SOURCE/HOST:	Synthetic.
PURITY:	≥98%
APPEARANCE:	Orange crystalline solid.
SOLUBILITY:	Soluble in water or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HAZARD:	VERY TOXIC. MAY BE TERATOGENIC. MAY BE CARCINOGENIC.

Product Description

More potent, lipophilic and antineoplastic analog of daunorubicin. DNA-damaging effect includes DNA oxidation and methylation, DNA intercalation, inhibition of DNA synthesis, induction of DNA strand breaks and delay of cell cycle progression. Inhibitor of topoisomerase IIα. Produces endonucleolytic cleavage and is a marker of apoptosis.

Product Specific Literature References

Idarubicin (4-demethoxydaunorubicin). A preliminary overview of preclinical and clinical studies: F. Ganzina, et al.; Invest. New Drugs 4, 85 (1986), Review Abstract
Antitumor activity of idarubicin, a derivative of daunorubicin, against drug sensitive and resistant P388 leukemia: T. Tsuruo, et al.; Anticancer Res. 13, 357 (1993) Abstract
Study of apoptosis-related responses of leukemic blast cells to in vitro anthracycline treatment: M.A. Belaud-Rotureau, et al.; Leukemia 14, 1266 (2000) Abstract
A comparison of the in vitro genotoxicity of anticancer drugs idarubicin and mitoxantrone: J. Blasiak, et al.; Acta Biochim. Pol. 49, 145 (2002) Abstract; Full Text
Repair of idarubicin-induced DNA damage: A cause of resistance?: D.C. Dartsch & F. Gieseler; DNA Repair (Amst) 6, 1618 (2007) Abstract

Further Categories Containing This Product:

Antibiotics - Topoisomerase Inhibitors • Antitumor Agents (Apoptosis Inducers) • Antitumor Antibiotics • Antitumor Agents (DNA Interaction & Gene Regulation)

ALX-380-273

Revised 03-Apr-08

Doxycycline . hyclate
SYNONYMS	Doxycycline . hydrochloride . hemiethanolate . hemihydrate
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antibiotics for Angiogenesis Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-273-G001		1 g	20.00 USD
ALX-380-273-G005		5 g	60.00 USD

Product Specification

FORMULA:	C₂₂H₂₄N₂O₈ . HCl . 0.5C₂H₆O . 0.5H₂O
MW:	444.4 . 36.5 . 23.0 . 9.0
CAS NUMBER:	24390-14-5
MERCK INDEX:	14: 3440
RTECS:	QI8925000
SOURCE/HOST:	Semisynthetic from oxytetracycline.
PURITY:	≥98% (Assay on dry basis)
APPEARANCE:	Yellow crystalline solid.
SOLUBILITY:	Soluble in water (50mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Hygroscopic.
HAZARD:	HARMFUL. MAY BE TERATOGENIC. MAY BE CARCINOGENIC.

Product Description

Broad spectrum antibiotic and bacteriostatic. Shows antiprotozoal properties. Potent inhibitor of MMPs (matrix metalloproteinases) in vivo. Inhibits collagen synthesis.

Product Specific Literature References

Alpha-6-deoxyoxytetracycline. I. Some biological properties: A.R. English; Proc. Soc. Exp. Biol. Med. 122, 1107 (1966) Abstract
A compilation of LD50 values in newborn and adult animals: E.I. Goldenthal; Toxicol. Appl. Pharmacol. 18, 185 (1971) Abstract
An efficacy trial of doxycycline chemoprophylaxis against leptospirosis: E.T. Takafuji, et al.; N. Engl. J. Med. 310, 497 (1984) Abstract
Specificity of the anticollagenase action of tetracyclines: relevance to their anti-inflammatory potential: K. Suomalainen, et al.; Antimicrob. Agents Chemother. 36, 227 (1992) Abstract
Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline: R. Hanemaaijer, et al.; J. Biol. Chem. 272, 31504 (1997) Abstract; Full Text
Doxycycline and tissue repair in rats: S. Lamparter, et al.; J. Lab. Clin. Med. 139, 295 (2002) Abstract
Molecular interactions between matrilysin and the matrix metalloproteinase inhibitor doxycycline investigated by deuterium exchange mass spectrometry: R.A. Garcia, et al.; Mol. Pharmacol. 67, 1128 (2005) Abstract; Full Text
Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices: C. Franco, et al.; Am. J. Pathol. 168, 1697 (2006) Abstract; Full Text
Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia: D. Burggraf, et al.; Neurobiol. Dis. 25, 506 (2007) Abstract

Further Categories Containing This Product:

Antitumor Agents (Anti-proliferative) • MMP Inhibitors • Antitumor Antibiotics • Antitumor Agents (Enzyme Inhibitors)

ALX-380-283

Revised 21-May-08

7-Amino-actinomycin D
SYNONYMS	7-AAD 7-Aminoactinomycin C₁
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-380-283-M001		1 mg	90.00 USD
ALX-380-283-M005		5 mg	360.00 USD

Product Specification

FORMULA:	C₆₂H₈₇N₁₃O₁₆
MW:	1270.4
CAS NUMBER:	7240-37-1
RTECS:	AU1579000
SOURCE/HOST:	Semisynthetic from actinomycin D (Prod. No. ALX-380-009).
PURITY:	≥96% (HPLC)
APPEARANCE:	Red to dark purple powder.
SOLUBILITY:	Soluble in DMSO (20mg/ml), methanol or 100% ethanol; slightly soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Hygroscopic.
HAZARD:	VERY TOXIC.

Product Description

Membrane impermeable fluorescent DNA intercalator. Used as a fluorescent DNA stain. Inhibitor of DNA-primed RNA polymerase and DNA polymerase. Induces apoptosis and is used as a tool to identify apoptotic cells. Cytochemical probe. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Has antibacterial properties.

Product Specific Literature References

7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties: J.E. Gill, et al.; J. Histochem. Cytochem. 23, 793 (1975) Abstract; Full Text
7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies: S.K. Sengupta, et al.; J. Med. Chem. 18, 1175 (1975) Abstract
Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells: P.S. Rabinovitch, et al.; J. Immunol. 136, 2769 (1986) Abstract
Binding specificities of actinomycin D to non-self-complementary -XGCY-tetranucleotide sequences: F.M. Chen; Biochemistry 31, 6223 (1992) Abstract
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry: I. Schmid, et al.; Cytometry 13, 204 (1992) Abstract
Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation: K. Toba, et al.; Cytometry 13, 60 (1992) Abstract
Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle: R.J. Berg, et al.; Carcinogenesis 14, 103 (1993) Abstract
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y: K. Toba, et al.; J. Immunol. Methods 182, 193 (1995) Abstract
Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect: X. Su, et al.; J. Immunol. 156, 4198 (1996) Abstract
The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques: N.J. Philpott, et al.; Blood 87, 2244 (1996) Abstract; Full Text
A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry: I. Schmid, et al.; J. Immunol. Methods 170, 145 (1996) Abstract
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods: H. Lecoeur, et al.; J. Immunol. Methods 209, 111 (1997) Abstract
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY: K. Toba, et al.; Eur. J. Haematol. 64, 10 (2000) Abstract
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007) Abstract

Related Products

ALX-380-009

Actinomycin D

Further Categories Containing This Product:

Antibiotics - Apoptosis Inducers & Inhibitors • Transcription Inhibitors / Related Products • Antitumor Agents (Apoptosis Inducers) • Apoptosis & Necrosis Detection Other Products • DNA Intercalators & Crosslinkers • Dyes / Stains / Fluorescent Probes

ALX-480-083

Revised 05-Jun-07

Puromycin aminonucleoside
SYNONYMS	3'-Amino-3'-deoxy-N,N-dimethyl-adenosine
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-480-083-M010		10 mg	50.00 USD
ALX-480-083-M050		50 mg	160.00 USD
ALX-480-083-M250		250 mg	590.00 USD

Product Specification

FORMULA:	C₁₂H₁₈N₆O₃
MW:	294.3
CAS NUMBER:	58-60-6
RTECS:	AU7337000
PURITY:	≥95%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HAZARD:	MAY BE TERATOGENIC. MAY BE MUTAGENIC.

Product Description

Antibiotic with antineoplastic properties. Does not inhibit protein synthesis or induce apoptosis. May cause nephrosis.

Product Specific Literature References

Puromycin aminonucleoside suppresses integrin expression in cultured glomerular epithelial cells: U. Krishnamurti, et al.; J. Am. Soc. Nephrol. 12, 758 (2001) Abstract

Further Categories Containing This Product:

Antitumor Agents (Anti-proliferative)

ALX-630-107

Revised 21-Feb-08

Bleomycin . sulfate
SYNONYMS	Blenoxane
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-630-107-M010		10 mg	155.00 USD
ALX-630-107-M050		50 mg	595.00 USD

Product Specification

FORMULA:	C₅₅H₈₂N₁₇O₂₁S₃ . H₂SO₄
MW:	1413.6 . 98.1
CAS NUMBER:	9041-93-4
MERCK INDEX:	14: 1318
RTECS:	EC5991990
SOURCE/HOST:	A group of related glycopeptide antibiotics isolated from Streptomyces verticillus.
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water (20mg/ml) or methanol.
ACTIVITY:	1'500-2'000 IU/mg (corresponds to 1.5-2.0 bleomycin units (USP)/mg)
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Solutions in normal saline (~2mg/ml) are stable for 3 months when stored at +4°C in a glass container or stable for 1 month when stored at +4°C in a PVC container. Solutions in water are stable for several days only.
HAZARD:	MAY BE CARCINOGENIC. TOXIC.

Product Description

Radiomimetic DNA-cleaving agent that produces double and single DNA strand breaks through an oxygen-radical-dependent mechanism. Inhibits intracellular DNA replication. DNA alkylating agent. Antineoplastic.

Product Specific Literature References

Bleomycin and other antitumor antibiotics of high molecular weight: H. Umezawa; Antimicrobial Agents Chemother. (Bethesda) 5, 1079 (1965) Abstract
Specificity of deoxyribonucleic acid cleavage by bleomycin, phleomycin, and tallysomycin: J. Kross, et al.; Biochemistry 21, 4310 (1982) Abstract
Chromatin structure during bleomycin-induced DNA damage and repair
: B.P. Cuiffo, et al.; J. Free Radic. Biol. Med. 1, 139 (1985) Abstract
Cleavage of tRNA by Fe(II)-bleomycin: A. Huttenhofer, et al.; J. Biol. Chem. 267, 24471 (1992) Abstract
Antiangiogenic chemotherapeutic agents: characterization in comparison to their tumor growth inhibition in human renal cell carcinoma models: M. Schirner, et al.; Clin. Cancer Res. 4, 1331 (1998) Abstract
Sequence-specific changes in the metal site of ferric bleomycin induced by the binding of DNA: J.W. Sam, et al.; J. Biol. Chem. 273, 16090 (1998) Abstract
Changes in c-Jun but not Bcl-2 family proteins in p53-dependent apoptosis of mouse cerebellar granule neurons induced by DNA damaging agent bleomycin: T. Araki, et al.; Brain Res. 794, 239 (1998) Abstract
Induction of apoptosis by bleomycin in resting and cycling human lymphocytes: P. Vernole, et al.; Mutagenesis 13, 209 (1998) Abstract
Synergistic Effects of Laserthermia and Bleomycin-sulfate on Micronuclei Formation in Cytokinesis-blocked HeLa Cells: A.Sh. Monfared & H. Mozdarani; Irn. J. Med. Sci. 24, 87 (1999)
Bleomycin: new perspectives on the mechanism of action: S.M. Hecht; J. Nat. Prod. 63, 158 (2000), (Review) Abstract
Bleomycin-induced alterations in DNA replication: relationship to DNA damage: J. Dziegielewski, et al.; Biochemistry 40, 704 (2001) Abstract

Further Categories Containing This Product:

Antitumor Agents (DNA Interaction & Gene Regulation) • DNA Fragmentation & Chromatin Condensation

ALX-804-624

Revised 20-Jun-05

Monoclonal Antibody to Doxorubicin (MAD11)
SYNONYMS	anti-DXR MAb (MAD11) anti-14-Hydroxydaunomycin MAb (MAD11) anti-Adriamycin MAb (MAD11)
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Antitumor Antibiotics

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-624-C100		100 µg	320.00 USD

Product Specification

CLONE:	MAD11
ISOTYPE:	Mouse IgG2
CONCENTRATION:
FORMULATION:	Liquid. Purified antibody containing 150mM sodium chloride and 0.02% sodium azide, pH 7.4.
IMMUNOGEN:	Doxorubicin-BSA.
SPECIFICITY:	Recognizes anthracycline compounds including doxorubicin analogs and derivatives.
APPLICATION:	ELISA Immunohistochemistry Functional Application: In vivo antidotic effect.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
HANDLING:	Do not freeze.

Product Specific Literature References

Monoclonal antibodies against doxorubicin: A. Balsari, et al.; Int. J. Cancer 42, 798 (1988) Abstract
A new monoclonal antibody recognizing anthracyclinic molecule: A. Balsari, et al.; Anticancer Res. 10, 129 (1990) Abstract
Anti-drug monoclonal antibodies antagonize toxic effect more than anti-tumor activity of doxorubicin: A. Balsari, et al.; Int. J. Cancer 47, 889 (1991) Abstract
An anti-doxorubicin monoclonal antibody modulates kinetic and dynamic characteristics of the drug: A. Sardini, et al.; Int. J. Cancer 50, 617 (1992) Abstract
Protection against doxorubicin-induced alopecia in rats by liposome-entrapped monoclonal antibodies: A.L. Balsari, et al.; FASEB J. 8, 226 (1994) Abstract
Effect of a bifunctional monoclonal antibody directed against a tumor marker and doxorubicin on the growth of epidermoid vulvar carcinoma grafted in athymic mice: D. Morelli, et al.; Cell Biophys. 24-25, 119 (1994) Abstract
Relevance of antibody valency in EGF receptor modulation: D. Morelli, et al.; Scand. J. Immunol. 39, 453 (1994) Abstract
Oral administration of anti-doxorubicin monoclonal antibody prevents chemotherapy-induced gastrointestinal toxicity in mice: D. Morelli, et al.; Cancer Res. 56, 2082 (1996) Abstract
Apoptosis of hair follicle cells during doxorubicin-induced alopecia in rats: R. Cece, et al.; Lab. Invest. 75, 601 (1996) Abstract
Intratibial injection of an anti-doxorubicin monoclonal antibody prevents drug-induced myelotoxicity in mice: D. Morelli, et al.; Br. J. Cancer 75, 656 (1997) Abstract
Topical administration of a doxorubicin-specific monoclonal antibody prevents drug-induced mouth apoptosis in mice: A. Balsari, et al.; Br. J. Cancer 85, 1964 (2001) Abstract
Role of proliferation in HER2 status predicted response to doxorubicin: M. Campiglio, et al.; Int. J. Cancer 105, 568 (2003) Abstract

Further Categories Containing This Product:

Antibiotics - Topoisomerase Inhibitors • Cancer Other Products • Topoisomerases Other Products • Monoclonal Antibodies