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p53 Antibodies
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ALX-804-186 Revised 04-Sep-07
Monoclonal Antibody to p53 (phosphorylated) (pSer392) (9F4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-186-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9F4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1 ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of p53 phosphorylated at Ser392.
SPECIFICITY: Recognizes activated human p53 phosphorylated at Ser392. Does not cross-react with the non-phosphorylated form of p53 or with unrelated serine-phosphorylated proteins.
APPLICATION: Western Blot: 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Further Categories Containing This Product:
Phospho-specific AntibodiesTumor MarkersMonoclonal Antibodies
 
 
ALX-801-060 Revised 20-May-08
Monoclonal Antibody to p53 (human) (BP53-12)
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SYNONYMS anti-p53 (human) MAb (BP53-12)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-060-C100   100 µg 210.00 USD Add To Cart
ALX-801-060F-C100 FITC 100 µg 315.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: BP53-12
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant human wild-type p53 protein.
SPECIFICITY: Antibody BP53-12 detects a denaturation resistant epitope (aa 16-25) in the human p53 protein.
APPLICATION: BP53-12:
ELISA
Immunocytochemistry
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1-2µg/ml)
BP53-12-FITC:
Flow Cytometry
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies: J. Bartek, et al.; J. Pathol. 169, 27 (1993) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 

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