ALEXIS Biochemicals: TPD52L1 (human) (recombinant) (His)

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ALX-804-360

Revised 05-Oct-05

Monoclonal Antibody to SnoN (1G1)
SYNONYMS	anti-Ski-related Novel Protein Non-Alu-containing MAb (1G1)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-360-C100		100 µg	340.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Others
CLONE:	1G1
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant SnoN (Ski-related novel protein non-Alu-containing).
SPECIFICITY:	Recognizes human and chicken SnoN. Detects a band of ~80kDa by Western blot.
APPLICATION:	Immunoprecipitation (5µg/sample) Western Blot (1-5µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 2 years after receipt when stored at -20°C.

Product Description

Ski [1] and SnoN [2] are oncoproteins and transcription factors that form both homodimers and heterodimers [3]. Both have the ability to transform avian fibroblasts and induce muscle differentiation [4,5]. They recognize a specific DNA binding site (GTCTAGAC) through which they repress transcription [6]. Ski and SnoN are components of a macromolecular complex containing the co-repressor N-CoR/SMRT, mSin3 and histone deacetylase. Ski and SnoN negatively regulate TGF-β signalling by recruiting this complex to Smads [7]. Interactions between Ski and Smad2, -3 and -4 blocks Smads-mediated transcriptional activation as well as TGF-β-induced growth suppression of epithelial cells, suggesting oncogenic effects of Ski derive in part through its ability to antagonize the growth-inhibitory signals initiated by members of the TGF-β family of ligands. For a review see [8].

Product Specific Literature References

[1] Generation of transforming viruses in cultures of chicken fibroblasts infected with an avian leukosis virus: E. Stavnezer, et al.; J. Virol. 39, 920 (1981)
[2] Isolation of human cDNA clones of ski and the ski-related gene, sno: N. Nomura, et al.; Nucl. Acids Res. 17, 5489 (1989) Abstract
[3] Heterodimers of the SnoN and Ski oncoproteins form preferentially over homodimers and are more potent transforming agents: S.B. Cohen, et al.; Nucl. Acids Res. 27, 1006 (1999)
[4] The ski oncogene induces muscle differentiation in quail embryo cells: C. Colmenares & E. Stavnezer; Cell 59, 293 (1989)
[5] Sequence and biological activity of chicken snoN cDNA clones: P.L. Boyer, et al.; Oncogene 8, 457 (1993)
[6] A domain necessary for the transforming activity of SnoN is required for specific DNA binding, transcriptional repression and interaction with TAF(II)110: S.B. Cohen, et al.; Oncogene 17, 2505 (1998) Abstract
[7] Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor:: T. Nomura, et al.; Genes Dev. 13, 412 (1999) Abstract
[8] Ski/Sno and TGF-beta signaling: X. Liu, et al.; Cytokine Growth Factor Rev. 12, 1 (2001), (Review) Abstract

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Transcription Factors Other Products • Monoclonal Antibodies

ALX-801-052

Revised 24-Oct-06

Monoclonal Antibody to TAG-72 (human) (B72.3)
SYNONYMS	anti-Tumor-associated Glycoprotein-72 (human) MAb (B72.3) anti-BRST-3 (human) MAb (B72.3) anti-CA 72.4 (human) MAb (B72.3)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-801-052-L001		1 ml	670.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	B72.3
ISOTYPE:	Mouse IgG1
FORMULATION:	Liquid. In 0.01M PBS containing 0.1% sodium azide and 1% BSA.
IMMUNOGEN:	Membrane-enriched fraction of a human breast carcinoma metastatic to the liver.
SPECIFICITY:	Recognizes human TAG-72 (tumor-associated glycoprotein-72).
APPLICATION:	Immunohistochemistry (paraffin sections (1:40)) Optimal conditions must be determined individually.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

TAG-72, a tumor-associated oncofetal antigen expressed by a wide variety of human adeno-carcinomas. This antigen is expressed by 84% of invasive ductal breast car-cinomas, and 85-90% of colon, pancreatic, gastric, esophageal, lung (non-small cell), ovarian and endometrial adenocarcinomas. It is not expressed by leukemias, lymphomas, sarcomas, mesotheliomas, melanomas or benign tu-mors. TAG-72 is also expressed on normal secretory endometrium, but not on other normal tissues.

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Monoclonal Antibodies

ALX-804-568

Revised 10-Mar-06

Monoclonal Antibody to TPD52L1 (human) (d1C5)
SYNONYMS	anti-D53 (human) MAb (d1C5)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-568-R050		50 µl	195.00 USD
ALX-804-568-R100		100 µl	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	d1C5
ISOTYPE:	Mouse IgG2b
CONCENTRATION:	1mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS, pH 7.4, containing 0.08% sodium azide.
IMMUNOGEN:	Recombinant human TPD52L1 (D53).
SPECIFICITY:	Recognizes human TPD52L1.
APPLICATION:	ELISA Immunohistochemistry Western Blot (1:500-1:2’000) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 1 month when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

General Information

TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signalling intermediates and regulators of vesicle trafficking.

General Literature References

Identification of homo- and heteromeric interactions between members of the breast carcinoma-associated D52 protein family using the yeast two-hybrid system: J.A. Byrne, et al.; Oncogene 16, 873 (1998) Abstract
Alternative splicing as a mechanism for regulating 14-3-3 binding: interactions between hD53 (TPD52L1) and 14-3-3 proteins: R. Boutros, et al.; J. Mol. Biol. 332, 675 (2003) Abstract

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Monoclonal Antibodies

ALX-804-650

Revised 25-Oct-06

Monoclonal Antibody to Trop-2 (human) (MOv16)
SYNONYMS	anti-Tumor-associated Calcium Signal Transducer 2 (human) MAb (MOv16)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Tumor Markers

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-650-C100		100 µg	295.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	MOv16
ISOTYPE:	Mouse IgG1
CONCENTRATION:	1mg/ml.
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In 10mM sodium phosphate buffer, pH 7.4, containing 150mM sodium chloride.
IMMUNOGEN:	Membrane preparation from human ovarian carcinoma containing human Trop-2 (tumor-associated calcium signal transducer 2).
SPECIFICITY:	Recognizes human Trop-2.
APPLICATION:	Flow Cytometry (1-10µg/ml) Immunocytochemistry (1-10µg/ml) Immunohistochemistry (frozen sections (1-10µg/ml)) Immunoprecipitation (1-10µg/ml) Western Blot (non-reducing (1-10µg/ml))
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Stable for at least 1 year when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Characterization of human ovarian carcinoma-associated antigens defined by novel monoclonal antibodies with tumor-restricted specificity: S. Miotti, et al.; Int. J. Cancer 39, 297 (1987) Abstract
Biochemical characterization of Trop-2, a cell surface molecule expressed by human carcinomas: formal proof that the monoclonal antibodies T16 and MOv-16 recognize Trop-2: S. Alberti, et al.; Hybridoma 11, 539 (1992) Abstract
Transformation of human keratinocytes is characterized by quantitative and qualitative alterations of the T-16 antigen (Trop-2, MOv-16): M.P. Schon and C.E. Orfanos; Int. J. Cancer 60, 88 (1995) Abstract
Human Trop-2 is a tumor-associated calcium signal transducer: E. Ripani, et al.; Int. J. Cancer 76, 671 (1998) Abstract
Effects of different immunolabeling techniques on the detection of small-cell lung cancer cells in bone marrow: G. Pelosi, et al.; J. Histochem. Cytochem. 47, 1075 (1999) Abstract; Full Text

General Information

Trop-2 (tumor-associated calcium signal transducer 2) [1,2] is a 46-48kDa cell surface glycoprotein that acts as a calcium signal transducer [3]. The molecule is expressed at high levels in human throphoblasts and overexpressed on a large variety of human carcinomas (including breast, colon, lung and prostate cancers). As an example, Trop-2 is among the most highly overexpressed genes in primary ovarian serous papillary tumors compared to normal ovarian epithelium [4] and in in colon tumors compared with normal mucosa [5]. Trop-2 is homologous to Trop-1 and both are homologous to serum IGF-II-binding proteins. The specific role of Trop-2 in normal and tumor cells is not fully defined, but accumulating evidence suggests a regulatory role in the growth of carcinoma cells. Furthermore recent data support the hypothesis that Trop-2 is involved in tumor invasion and metastasis. Indeed, TROP2 is one of the cancer-related genes that correlates with biological aggressiveness and poor prognosis of colorectal cancer [6].

General Literature References

[1] Human trophoblast cell-surface antigens defined by monoclonal antibodies: M. Lipinski, et al.; PNAS 78, 5147 (1981) Abstract
[2] Biochemical characterization of Trop-2, a cell surface molecule expressed by human carcinomas: formal proof that the monoclonal antibodies T16 and MOv-16 recognize Trop-2: S. Alberti, et al.; Hybridoma 11, 539 (1992) Abstract
[3] Human Trop-2 is a tumor-associated calcium signal transducer: E. Ripani, et al.; Int. J. Cancer 76, 671 (1998) Abstract
[4] In silico data filtering to identify new angiogenesis targets from a large in vitro gene profiling data set: M.E. Gerritsen, et al.; Physiol. Genomics 10, 13 (2002) Abstract
[5] Gene expression profiles in primary ovarian serous papillary tumors and normal ovarian epithelium: identification of candidate molecular markers for ovarian cancer diagnosis and therapy: A.D. Santin, et al.; Int. J. Cancer 112, 14 (2004) Abstract
[6] Clinical significance of TROP2 expression in colorectal cancer: T. Ohmachi, et al.; Clin. Cancer Res. 12, 3057 (2006) Abstract

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Monoclonal Antibodies

ALX-210-356

Revised 20-Jun-07

Polyclonal Antibody to α-Actinin-4
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Actin/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-356-C050		50 µg	490.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Pig Others
SOURCE/HOST:	From rabbit.
CONCENTRATION:	250µg/ml
PURITY DETAIL:	Epitope-affinity purified.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.01% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 28-38 (M²⁸GDYMAQEDDW³⁸) of human and rat actinin-4 and to aa 29-39 of mouse actinin-4.
SPECIFICITY:	Recognizes human, mouse, rat, pig, and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3.
APPLICATION:	Immunohistochemistry (frozen sections (1µg/ml), paraffin sections [3]) Immunoprecipitation (25µg/ml) Western Blot (0.1-0.25µg/ml) The recommended dilutions refer to the analysis of mouse cells and tissues with intermediate levels of actinin-4 expression and must be viewed as approximate.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
POSITIVE CONTROL:	Human platelet protein (500µg) (Prod. No. ALX-840-007), supplied at 5mg/ml in SDS sample buffer (100mM NaCl, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10µg/ml Bromophenol Blue sodium salt). Use 5µl (25µg) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.

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Product Specific Literature References

[1] Expression of alpha-actinin-4 in acquired human nephrotic syndrome: a quantitative immunoelectron microscopy study: N.P. Goode, et al.; Nephrol. Dial. Transplant. 19, 844 (2004) Abstract; Full Text
[2] CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling: Q. Yan, et al.; Mol. Biol. Cell 16, 2470 (2005) Abstract; Full Text
[3] Podocyte involvement in human immune crescentic glomerulonephritis: J. Bariety, et al.; Kidney Int. 68, 1109 (2005) Abstract

General Information

α-Actinin-4 is an actin-bundling protein of ~100kDa that is associated with cell motility, endocytosis, and cancer invasion [1, 2]. The α-actinin family comprises two non-muscle isoforms (α-actinin-1 and -4) and two skeletal muscle isoforms (α-actinin-2 and -3), with α-actinin-2 being also expressed in cardiac muscle [3]. While α-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus [1]. Mutations in the α-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS) [4], which is thought to result from a defect in glomerular podocyte function. A point mutation in the α-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma [5]. α-actinin-4 interacts with a variety of proteins, including the ring finger protein BERP [6], the PDZ-LIM protein CLP-36 [7], the hemidesmosomal and cell-cell contact protein BP180 [8], and the tight junction protein MAGI-1 [9]. Moreover, α-actinin-4 forms a ternary complex with Ca²⁺/ Calmodulin-dependent protein kinase II and Densin-180, a protein of postsynaptic densities in CNS neurons [10]. Ca²⁺-dependent association of α-actinin-4 with E3KARP is required for Ca²⁺-dependent inhibition of the Na⁺/ H⁺exchanger 3 (NHE3) [11].

General Literature References

[1] Actinin-4, a novel actin-bundling protein associated with cell motility and cancer invasion: K. Honda, et al.; J. Cell Biol. 140, 1383 (1998) Abstract
[2] Alpha-Actinins: D.R. Critchley & G. Flood; in: Guidebook to the Cytoskeletal and Motor Proteins, T. Kreis & R. Vale, Editors, Oxford University Press: Oxford p. 24 (1999)
[3] Actinin-4 is preferentially involved in circular ruffling and macropinocytosis in mouse macrophages: analysis by fluorescence ratio imaging: N. Araki, et al.; J. Cell Sci. 113, 3329 (2000) Abstract
[4] Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis: J.M. Kaplan, et al.; Nat. Genet. 24, 251 (2000) Abstract
[5] BERP, a novel ring finger protein, binds to alpha-actinin-4: A.E. El-Husseini, et al.; BBRC 267, 906 (2000) Abstract
[6] CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4: T. Vallenius, et al.; J. Biol. Chem. 275, 11100 (2000) Abstract
[7] A point mutation in the alpha-actinin-4 gene generates an antigenic peptide recognized by autologous cytolytic T lymphocytes on a human lung carcinoma: H. Echchakir, et al.; Cancer Res. 61, 4078 (2001) Abstract
[8] Interactions of a hemidesmosome component and actinin family members: A.M. Gonzalez, et al.; J. Cell Sci. 114, 4197 (2001) Abstract
[9] Densin-180 forms a ternary complex with the (alpha)-subunit of Ca2+/calmodulin-dependent protein kinase II and (alpha)-actinin: R.S. Walikonis, et al.; J. Neurosci. 21, 423 (2001) Abstract
[10] Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1: K.M. Patrie, et al.; J. Biol. Chem. 277, 30183 (2002) Abstract
[11] Ca(2+)-dependent inhibition of Na+/H+ exchanger 3 (NHE3) requires an NHE3-E3KARP-alpha-actinin-4 complex for oligomerization and endocytosis: J.H. Kim, et al.; J. Biol. Chem. 277, 23714 (2002) Abstract

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Endocytosis • Polyclonal Antibodies • Tumor Markers

ALX-210-151

Revised 19-Mar-08

Polyclonal Antibody to Androgen Receptor
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Androgens & Androgen Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-151-R200		200 µl	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Monkey
SOURCE/HOST:	From rabbit.
FORMULATION:	Liquid. Antiserum diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:	Recombinant human androgen receptor (aa 331-572).
SPECIFICITY:	Recognizes human, mouse, rat and monkey androgen receptor. Detects a band of ~110kDa by Western blot.
APPLICATION:	Immunocytochemistry Immunohistochemistry (paraffin sections, frozen sections) (1:100) Immunoprecipitation Western Blot (1:500)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Polyclonal Antibodies • Tumor Markers

ALX-210-152/1

Revised 28-Feb-08

Polyclonal Antibody to Androgen Receptor
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Androgens & Androgen Receptors/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-152/1-C100		100 µg	379.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
PURITY DETAIL:	Epitope affinity-purified IgG.
FORMULATION:	Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to N-terminal aa 1-21 (M¹EVQLGLGRVYPRPPSKTYRG²¹C) of human, mouse and rat androgen receptor.
SPECIFICITY:	Recognizes human androgen receptor. Detects a band of ~110kDa by Western blot. Does not cross-react with estrogen, progesterone, or glucocorticoid receptors.
APPLICATION:	Western Blot (2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:	For Blocking Peptide see Prod. No. ALX-151-033.

Product Specific Literature References

Immunocytochemical analysis of androgen receptor along the ducts of the separate rat prostate lobes after androgen withdrawal and replacement: G.S. Prins, et al.; Endocrinology 132, 169 (1993) Abstract
Androgen receptor localization in different cell types of the adult rat prostate: G.S. Prins, et al.; Endocrinology 129, 3187 (1991) Abstract

SPECIES CROSSREACTIVITY:	Human Mouse Rat
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml
PURITY:	≥90%
PURITY DETAIL:	Protein A-affinity purified.
FORMULATION:	Liquid. In TRIS/glycine, pH 8.0, containing 0.01% sodium azide.
IMMUNOGEN:	Recombinant FNIII repeats 7-9 of mouse tenascin-W.
SPECIFICITY:	Recognizes human, mouse and rat tenascin-W.
APPLICATION:	ELISA (1:10’000) Immunocytochemistry (1:1’000) Immunohistochemistry (frozen sections, paraffin sections (1:500)) Immunoprecipitation Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.