ALEXIS Biochemicals: TIMP-2 (human)

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ALX-200-420

Revised 18-Mar-08

MMP-2/TIMP-2 Proenzyme Complex (human)
SYNONYMS	Matrix Metalloproteinase 2/Tissue Inhibitor of Metalloproteinase 2 Complex (human)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	MMPs

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-420-C005		5 µg	166.00 USD

Product Specification

EC:	3.4.24.24 (MMP-2)
SOURCE/HOST:	Isolated from human rheumatoid synovial fibroblasts. Requires activation.
CONCENTRATION:	~100µg/ml
PURITY:	≥95% (SDS-PAGE, Western blot)
PURITY DETAIL:	No other MMP contaminants are detectable.
FORMULATION:	Liquid. In 50mM TRIS-HCl pH 7.0, 200mM sodium chloride, 5mM CaCl₂, 1µM ZnCl₂, 0.05% NaN₃ and 0.05% BRIJ 35.
SPECIFIC ACTIVITY:	≥30mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Do always keep the enzyme on ice.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
The complex between a tissue inhibitor of metalloproteinases (TIMP-2) and 72-kDa progelatinase is a metalloproteinase inhibitor: H. Kolkenbrock, et al.; Eur. J. Biochem. 198, 775 (1991) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Activation of progelatinase A and progelatinase A/TIMP-2 complex by membrane type 2-matrix metalloproteinase: H. Kolkenbrock, et al.; Biol. Chem. 378, 71 (1997) Abstract

General Information

MMP-2 and TIMP-2 form a stable, but non-covalent 1:1 stoichiometric complex. The complex inhibits active matrix MMP's like collagenases and gelatinases and shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate).

BACKGROUND/TECHNICAL INFORMATION

Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) or 1mM mersalylic acid for 60-120 min. at 37°C. Do not use trypsin for activation! Do not dilute the enzyme for activation!

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link: MMP-2 (human) (precursor) (P08253), TIMP-2 (human) (precursor) (P16035)

Further Categories Containing This Product:

Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products • Natural Proteins • Enzymes

ALX-200-425

Revised 04-Mar-05

MMP-9/Lipocalin/TIMP-1 Complex (human)
SYNONYMS	Matrix Metalloproteinase 9/Lipocalin/Tissue Inhibitor of Metalloproteinase 1 Complex (human)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	MMPs

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-425-C005		5 µg	166.00 USD

Product Specification

EC:	3.4.24.35 (MMP-9)
SOURCE/HOST:	Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
PURITY:	≥95% (SDS-PAGE, Western blot)
FORMULATION:	Liquid. 5µg protein in 200mM NaCl, 50mM Tris-HCl pH 7.0, 5mM CaCl₂, 1µM ZnCl₂, 0.05% BRIJ35 and 0.05% sodium azide.
SPECIFIC ACTIVITY:	≥400mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0 (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

This product represents the ternary complex of the MMP-9 monomer, lipocalin and TIMP-1 and behaves like the progelatinase B/TIMP-1 complex, that inhibits active MMP's and, after activation acts as a gelatinase with reduced activity.

Product Specific Literature References

Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract

General Information

MMP-9 hydrolyzes the extracellular matrix, e.g. collagen types IV, V and IX and gelatin.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link: MMP-9 (human) (precursor) (P14780), Lipocalin (human) (precursor) (P80188), TIMP-1 (human) (precursor) (P01033)

Further Categories Containing This Product:

Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products • Natural Proteins • Enzymes

ALX-385-026

Revised 07-Oct-08

Nobiletin
SYNONYMS	3’,4’,5,6,7,8-Hexamethoxyflavone 2-(3,4-Dimethoxyphenyl)-5,6,7,8-tetramethoxy-4H-1-benzopyran-4-one CCRIS 9012 NSC 76751
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Flavones

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-385-026-M010		10 mg	390.00 USD

Product Specification

FORMULA:	C₂₁H₂₂O₈
MW:	402.4
CAS NUMBER:	478-01-3
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in methanol; sparingly soluble in water or ether.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Antioxidant and anti-inflammatory. Inhibits the phosphorylation of mitogen-activated protein kinase MEK. Suppresses the expression of matrix metalloproteinases (MMP) 1, 3 and 9, that are involved in the breakdown of the extracellular matrix during tumor metastasis. Suppresses NF-κB transcriptional activation, nitric oxide (NO) and PGE₂ production, inducible nitric oxide synthase (iNOS; NOS II) and cyclooxygenase-2 (COX-2) expression.

Product Specific Literature References

The citrus flavonoid, nobiletin, inhibits peritoneal dissemination of human gastric carcinoma in SCID mice: A. Minagawa, et al.; Jpn. J. Cancer Res. 92, 1322 (2001) Abstract
Novel anti-inflammatory actions of nobiletin, a citrus polymethoxy flavonoid, on human synovial fibroblasts and mouse macrophages: N. Lin, et al.; Biochem. Pharmacol. 65, 2065 (2003) Abstract
Inhibition of cell proliferation by nobiletin, a dietary phytochemical, associated with apoptosis and characteristic gene expression, but lack of effect on early rat hepatocarcinogenesis in vivo: H. Ohnishi, et al.; Cancer Sci. 95, 936 (2004) Abstract; Full Text
Nobiletin from citrus fruit peel inhibits the DNA-binding activity of NF-kappaB and ROS production in LPS-activated RAW 264.7 cells: S.Y. Choi, et al.; J. Ethnopharmacol. 113, 149 (2007) Abstract
Tangeretin and nobiletin induce G1 cell cycle arrest but not apoptosis in human breast and colon cancer cells: K.L. Morley, et al.; Cancer Lett. 251, 168 (2007) Abstract
Nobiletin, a citrus flavonoid that improves memory impairment, rescues bulbectomy-induced cholinergic neurodegeneration in mice: A. Nakajima, et al.; J. Pharmacol. Sci. 105, 122 (2007) Abstract; Full Text
Nobiletin enhances differentiation and lipolysis of 3T3-L1 adipocytes: T. Saito, et al.; BBRC 357, 371 (2007) Abstract
Protective effects of citrus nobiletin and auraptene in transgenic rats developing adenocarcinoma of the prostate (TRAP) and human prostate carcinoma cells: M. Tang, et al.; Cancer Sci. 98, 471 (2007) Abstract
Nobiletin, a citrus flavonoid with neurotrophic action, augments protein kinase A-mediated phosphorylation of the AMPA receptor subunit, GluR1, and the postsynaptic receptor response to glutamate in murine hippocampus: K. Matsuzaki, et al.; Eur. J. Pharmacol. 578, 194 (2008) Abstract
A citrus polymethoxyflavonoid, nobiletin, is a novel MEK inhibitor that exhibits antitumor metastasis in human fibrosarcoma HT-1080 cells: Y. Miyata, et al.; BBRC 366, 168 (2008) Abstract

Further Categories Containing This Product:

Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products • Natural Products - Nitric Oxide Pathway Modulators • Natural Products - Anti-inflammatory Agents • Natural Products - NF-kB Pathway Inhibitors • COX Inhibitors

ALX-200-426

Revised 26-Jun-08

TIMP-1 (human)
SYNONYMS	Tissue Inhibitor of Metalloproteinase 1 (human)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-426-C005		5 µg	197.00 USD

Product Specification

MW:	~28kDa.
SOURCE/HOST:	Isolated from stimulated human neutrophils. The secreted protein consists of 184 amino acids, six disulfide bonds and two glycosylation sites containing N-linked oligosaccharides.
CONCENTRATION:	80µg/ml
PURITY:	≥92% (SDS-PAGE, Western blot)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7.0, 200mM NaCl, 5mM CaCl₂, 1µM ZnCl₂, 0.05% BRIJ 35 and 0.05% sodium azide.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Description

Specific inhibitor of matrix metalloproteinases (MMPs). It has no activity on mammalian membrane metalloproteinases, procollagen peptidase or on bacterial metalloproteinases such as thermolysin.

Product Specific Literature References

The interaction of purified rabbit bone collagenase with purified rabbit bone metalloproteinase inhibitor: T.E. Cawston, et al.; Biochem. J. 211, 313 (1983) Abstract
Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes: G. Murphy, et al.; Biochem. J. 248, 265 (1987) Abstract
Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family: W.G. Stettler-Stevenson, et al.; J. Biol. Chem. 264, 17374 (1989) Abstract; Full Text
Generation and activity of the ternary gelatinase B/TIMP-1/LMW-stromelysin-1 complex: H. Kolkenbrock, et al.; Biol. Chem. 376, 495 (1995) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract
Shedding of the Transferrin Receptor Is Mediated Constitutively by an Integral Membrane Metalloprotease Sensitive to Tumor Necrosis Factor alpha Protease Inhibitor-2: M. Kaup, et al.; J. Biol. Chem. 277, 38494 (2002) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: It is recommended to perform a preincubation of ~20 min. at 37°C to allow formation of the enzyme-inhibitor complex.

Swiss-Prot link P01033: TIMP-1 (human) (precursor)
AfCS Signalling Gateway link A002285: TIMP-1 (mouse)

Further Categories Containing This Product:

Natural Proteins

ALX-200-427

Revised 25-Apr-08

TIMP-2 (human)
SYNONYMS	Tissue Inhibitor of Metalloproteinase 2 (human)
PRODUCT LINE	Cytoskeleton
PRODUCT CATEGORY	Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-427-C005		5 µg	212.00 USD

Product Specification

MW:	~21kDa (non-reduced); ~24kDa (reduced) (SDS-PAGE).
SOURCE/HOST:	Isolated from human fibroblasts.
CONCENTRATION:	40μg/ml
PURITY:	≥90% (SDS-PAGE, Western blot)
FORMULATION:	Liquid. In 50mM TRIS-HCl, pH 7, containing 200mM NaCl, 5mM CaCl₂, 1µM ZnCl₂, 0.05% BRIJ 35 and 0.05% sodium azide.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

Product Images

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Product Description

Glycoprotein that belongs to a family which takes part in the activation and regulation of the MMP-activity. Forms a non-covalent stoichiometric complex with latent and active MMP's. Inhibits the activities of MMP-1, MMP-2, MMP-12 and transin.

Product Specific Literature References

Purification and characterization of a two-chain form of tissue inhibitor of metalloproteinases (TIMP) type 2 and a low molecular weight TIMP-like protein: K. Miyazaki, et al.; J. Biol. Chem. 268, 14387 (1993) Abstract; Full Text
Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4: J. Greene, et al.; J. Biol. Chem. 271, 30375 (1996) Abstract; Full Text
Shedding of the Transferrin Receptor Is Mediated Constitutively by an Integral Membrane Metalloprotease Sensitive to Tumor Necrosis Factor alpha Protease Inhibitor-2: M. Kaup, et al.; J. Biol. Chem. 277, 38494 (2002) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: It is recommended to perform a preincubation of about 20min at 37°C which allows the formation of the enzyme-inhibitor complex.

Swiss-Prot link P16035: TIMP-2 (human) (precursor)
AfCS Signalling Gateway A002286: TIMP-2 (mouse)

Further Categories Containing This Product:

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