ALEXIS Biochemicals: PPM-18

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ALX-350-014

Revised 03-Apr-08

Calyculin A
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Other Signal Transduction Pathway Modulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-014-C010		10 µg	65.00 USD
ALX-350-014-C025		25 µg	110.00 USD
ALX-350-014-C050		50 µg	190.00 USD
ALX-350-014-C100		100 µg	320.00 USD

Product Specification

FORMULA:	C₅₀H₈₁N₄O₁₅P
MW:	1009.2
CAS NUMBER:	101932-71-2
SOURCE/HOST:	Isolated from Discodermia calyx.
PURITY:	≥95%
APPEARANCE:	Lyophilized.
SOLUBILITY:	Soluble in DMSO, 100% ethanol, methanol, acetonitrile, chloroform, methylene chloride or benzene. Insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	May by subject to isomerization when in solution. Biological activity is not affected by isomerization.
HANDLING:	Protect from light and moisture.
HAZARD:	TOXIC. MAY BE CARCINOGENIC.

Product Description

Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis.

Product Specific Literature References

Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS 85, 1768 (1988) Abstract
Protein phosphatases come of age: P.Cohen & P.T. Cohen; J. Biol. Chem. 264, 21435 (1989) Abstract; Full Text
Calyculin A and okadaic acid: inhibitors of protein phosphatase activity: H. Ishihara, et al.; BBRC 159, 871 (1989) Abstract
The structure and regulation of protein phosphatases: P. Cohen; Ann. Rev. Biochem. 58, 453 (1989) Abstract
Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin: M. Suganuma, et al.; Cancer Res. 50, 3521 (1990) Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C: R. Gopalakrishna; BBRC 189, 950 (1992) Abstract
Comparative effects of protein phosphatase inhibitors (okadaic acid and calyculin A) on human leukemia HL60, HL60/ADR and K562 cells: K. Sakurada, et al.; BBRC 187, 488 (1992) Abstract
Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis: Q. Song & M.F. Lavin; BBRC 190, 47 (1993) Abstract
Enhancement of AMPA-mediated synaptic transmission by the protein phosphatase inhibitor calyculin A in rat hippocampal slices: A. Figurov, et al.; Eur. J. Neurosci. 5, 1035 (1993) Abstract
Calyculin A, a non-phorbol ester type tumor promotor, induced oxidative DNA damage in stimulated human neutrophil-like cells: T. Takeuchi, et al.; BBRC 205, 1803 (1994) Abstract
Potassium and calcium channel involvement in induction of long-lasting synaptic enhancement by calyculin A, a protein phosphatase inhibitor, in rat hippocampal CA1 region: N. Murakami, et al.; Neurosci. Lett. 176, 181 (1994) Abstract
Differential toxicity of the protein phosphatase inhibitors microcystin and calyculin A: M.T. Runnegar, et al.; J. Pharmacol. Exp. Ther. 273, 545 (1995) Abstract
Inhibition of p130cas tyrosine phosphorylation by calyculin A: W. Qiu, et al.; J. Leukoc. Biol. 63, 631 (1998) Abstract
Unique features of the okadaic acid activity class of tumor promoters: H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol. 125, 150 (1999), Review Abstract
Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo: G. Tzivion, et al.; J. Biol. Chem. 275, 29772 (2000) Abstract
Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells: H. Tanaka, et al.; Int. J. Oncol. 31, 389 (2007) Abstract

Further Categories Containing This Product:

ALX-210-462

Revised 10-Apr-08

Polyclonal Antibody to TRB-3 (human)
SYNONYMS	anti-Tribbles Homolog 3 (human) PAb anti-Neuronal Cell Death-inducible Putative Kinase (human) PAb anti-p65-interacting Inhibitor of NF-κB (human) PAb anti-SINK (human) PAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	NF-kB Pathway Modulators

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-462-C100		100 µg	310.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SOURCE/HOST:	From rabbit.
CONCENTRATION:	1mg/ml.
PURITY DETAIL:	Purified IgG.
FORMULATION:	Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:	Synthetic peptide corresponding to aa 112-125 (A¹¹²RLPPHKHVARPTE¹²⁵) of human TRB-3 (tribbles homolog 3).
SPECIFICITY:	Recognizes human TRB-3.
APPLICATION:	ELISA (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry (paraffin sections(1:500)) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for 1-2 weeks when stored at +4°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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General Information

Tribbles homolog 3 (TRB-3) is a pseudokinase, shown to act as a potent negative regulator of PPARγ, a master regulator of adipocyte differentiation, and to control adipogenesis.
MANUFACTURER	Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

PI(3)K-Akt-mTOR Pathway Other Products • MAPK Pathway Other Products • Polyclonal Antibodies • Transcription Regulators Other Products

ALX-270-458

Revised 15-Jan-08

PPM-18
SYNONYMS	2-Benzoylamino-1,4-naphthoquinone
PRODUCT LINE	Nitric Oxide Pathway
PRODUCT CATEGORY	NOS Inhibitors (NOS Induction & Enzyme Activity)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-458-M010		10 mg	140.00 USD

Product Specification

FORMULA:	C₁₇H₁₁NO₃
MW:	277.3
CAS NUMBER:	65240-86-0
PURITY:	≥97% (HPLC)
APPEARANCE:	Yellow to orange solid.
SOLUBILITY:	Soluble in DMSO or acetonitrile.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 3 years after receipt when stored at -20°C.
HANDLING:	Packaged under inert gas. Protect from light. After reconstitution, prepare aliquots and store at -20°C.

Product Description

Cell permeable, anti-inflammatory agent that inhibits expression of iNOS (NOS II) (IC₅₀~5mM). Blocks activation of NF-κB in vitro and in vivo. Does not directly affect enzymatic activities of iNOS (NOS II) or eNOS (NOS III).

Product Specific Literature References

Synthesis and antiplatelet, antiinflammatory and antiallergic activities of 2,3-disubstituted 1,4-naphthoquinones: J.C. Lien, et al.; Chem. Pharm. Bull. (Tokyo) 44, 1181 (1996) Abstract
Inhibition of nitric oxide synthase expression by PPM-18, a novel anti-inflammatory agent, in vitro and in vivo: S.M. Yu, et al.; Biochem. J. 328, 363 (1997) Abstract; Full Text
Shear stress regulates endothelial nitric-oxide synthase promoter activity through nuclear factor kappaB binding: M.E. Davis, et al.; J. Biol. Chem. 279, 163 (2004) Abstract; Full Text

Further Categories Containing This Product:

NF-kB Pathway Modulators • Anti-inflammatory Agents Other Products

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