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Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products
You are here: Product Lines > Cancer > Matrix Metalloproteinases [MMPs] / Related Products > Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products
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ALX-200-420 Revised 18-Mar-08
MMP-2/TIMP-2 Proenzyme Complex (human)
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SYNONYMS Matrix Metalloproteinase 2/Tissue Inhibitor of Metalloproteinase 2 Complex (human)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-420-C005   5 µg 166.00 USD Add To Cart
Product Specification
EC: 3.4.24.24 (MMP-2)
SOURCE/HOST: Isolated from human rheumatoid synovial fibroblasts. Requires activation.
CONCENTRATION: ~100µg/ml
PURITY: ≥95% (SDS-PAGE, Western blot)
PURITY DETAIL: No other MMP contaminants are detectable.
FORMULATION: Liquid. In 50mM TRIS-HCl pH 7.0, 200mM sodium chloride, 5mM CaCl2, 1µM ZnCl2, 0.05% NaN3 and 0.05% BRIJ 35.
SPECIFIC ACTIVITY: ≥30mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Do always keep the enzyme on ice.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
The complex between a tissue inhibitor of metalloproteinases (TIMP-2) and 72-kDa progelatinase is a metalloproteinase inhibitor: H. Kolkenbrock, et al.; Eur. J. Biochem. 198, 775 (1991) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Activation of progelatinase A and progelatinase A/TIMP-2 complex by membrane type 2-matrix metalloproteinase: H. Kolkenbrock, et al.; Biol. Chem. 378, 71 (1997) Abstract
General Information
MMP-2 and TIMP-2 form a stable, but non-covalent 1:1 stoichiometric complex. The complex inhibits active matrix MMP's like collagenases and gelatinases and shows proteolytic activity after activation with APMA (4-aminophenylmercury acetate).
BACKGROUND/TECHNICAL INFORMATION Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) or 1mM mersalylic acid for 60-120 min. at 37°C. Do not use trypsin for activation! Do not dilute the enzyme for activation!

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link: MMP-2 (human) (precursor) (P08253), TIMP-2 (human) (precursor) (P16035)
Further Categories Containing This Product:
Natural Proteins • Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products • Enzymes
 
 
ALX-200-425 Revised 04-Mar-05
MMP-9/Lipocalin/TIMP-1 Complex (human)
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SYNONYMS Matrix Metalloproteinase 9/Lipocalin/Tissue Inhibitor of Metalloproteinase 1 Complex (human)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-425-C005   5 µg 166.00 USD Add To Cart
Product Specification
EC: 3.4.24.35 (MMP-9)
SOURCE/HOST: Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
PURITY: ≥95% (SDS-PAGE, Western blot)
FORMULATION: Liquid. 5µg protein in 200mM NaCl, 50mM Tris-HCl pH 7.0, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ35 and 0.05% sodium azide.
SPECIFIC ACTIVITY: ≥400mU/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0 (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
This product represents the ternary complex of the MMP-9 monomer, lipocalin and TIMP-1 and behaves like the progelatinase B/TIMP-1 complex, that inhibits active MMP's and, after activation acts as a gelatinase with reduced activity.
Product Specific Literature References
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract
General Information
MMP-9 hydrolyzes the extracellular matrix, e.g. collagen types IV, V and IX and gelatin.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link: MMP-9 (human) (precursor) (P14780), Lipocalin (human) (precursor) (P80188), TIMP-1 (human) (precursor) (P01033)
Further Categories Containing This Product:
Natural Proteins • Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products • Enzymes
 
 
ALX-200-426 Revised 26-Jun-08
TIMP-1 (human)
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SYNONYMS Tissue Inhibitor of Metalloproteinase 1 (human)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-426-C005   5 µg 197.00 USD Add To Cart
Product Specification
MW: ~28kDa.
SOURCE/HOST: Isolated from stimulated human neutrophils. The secreted protein consists of 184 amino acids, six disulfide bonds and two glycosylation sites containing N-linked oligosaccharides.
CONCENTRATION: 80µg/ml
PURITY: ≥92% (SDS-PAGE, Western blot)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.0, 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ 35 and 0.05% sodium azide.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Description
Specific inhibitor of matrix metalloproteinases (MMPs). It has no activity on mammalian membrane metalloproteinases, procollagen peptidase or on bacterial metalloproteinases such as thermolysin.
Product Specific Literature References
The interaction of purified rabbit bone collagenase with purified rabbit bone metalloproteinase inhibitor: T.E. Cawston, et al.; Biochem. J. 211, 313 (1983) Abstract
Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes: G. Murphy, et al.; Biochem. J. 248, 265 (1987) Abstract
Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family: W.G. Stettler-Stevenson, et al.; J. Biol. Chem. 264, 17374 (1989) Abstract; Full Text
Generation and activity of the ternary gelatinase B/TIMP-1/LMW-stromelysin-1 complex: H. Kolkenbrock, et al.; Biol. Chem. 376, 495 (1995) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract
Shedding of the Transferrin Receptor Is Mediated Constitutively by an Integral Membrane Metalloprotease Sensitive to Tumor Necrosis Factor alpha Protease Inhibitor-2: M. Kaup, et al.; J. Biol. Chem. 277, 38494 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Note: It is recommended to perform a preincubation of ~20 min. at 37°C to allow formation of the enzyme-inhibitor complex.

Swiss-Prot link P01033: TIMP-1 (human) (precursor)
AfCS Signalling Gateway link A002285: TIMP-1 (mouse)
Further Categories Containing This Product:
Natural Proteins
 
 
ALX-200-427 Revised 25-Apr-08
TIMP-2 (human)
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SYNONYMS Tissue Inhibitor of Metalloproteinase 2 (human)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Tissue Inhibitors of Matrix Metalloproteinases [TIMPs] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-200-427-C005   5 µg 212.00 USD Add To Cart
Product Specification
MW: ~21kDa (non-reduced); ~24kDa (reduced) (SDS-PAGE).
SOURCE/HOST: Isolated from human fibroblasts.
CONCENTRATION: 40μg/ml
PURITY: ≥90% (SDS-PAGE, Western blot)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7, containing 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ 35 and 0.05% sodium azide.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
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Product Description
Glycoprotein that belongs to a family which takes part in the activation and regulation of the MMP-activity. Forms a non-covalent stoichiometric complex with latent and active MMP's. Inhibits the activities of MMP-1, MMP-2, MMP-12 and transin.
Product Specific Literature References
Purification and characterization of a two-chain form of tissue inhibitor of metalloproteinases (TIMP) type 2 and a low molecular weight TIMP-like protein: K. Miyazaki, et al.; J. Biol. Chem. 268, 14387 (1993) Abstract; Full Text
Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4: J. Greene, et al.; J. Biol. Chem. 271, 30375 (1996) Abstract; Full Text
Shedding of the Transferrin Receptor Is Mediated Constitutively by an Integral Membrane Metalloprotease Sensitive to Tumor Necrosis Factor alpha Protease Inhibitor-2: M. Kaup, et al.; J. Biol. Chem. 277, 38494 (2002) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Note: It is recommended to perform a preincubation of about 20min at 37°C which allows the formation of the enzyme-inhibitor complex.

Swiss-Prot link P16035: TIMP-2 (human) (precursor)
AfCS Signalling Gateway A002286: TIMP-2 (mouse)
Further Categories Containing This Product:
Natural Proteins
 
 
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