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ALX-201-240
Revised 25-Jan-07
Elafin (human) (recombinant)
SYNONYMS
ESI (human) (recombinant)
Elastase-specific Inhibitor (human) (recombinant)
SKALP (human) (recombinant)
Skin-derived Antileukoproteinase (human) (recombinant)
Protease Inhibitor WAP3 (human) (recombinant)
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Antimicrobial Peptides & Proteins/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-240-C100
100 µg
275.00 USD
Product Specification
MW:
~6kDa
SOURCE/HOST:
Produced in yeast.
PURITY:
≥90% (HPLC, SDS-PAGE)
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute in sterile distilled water. Further dilutions should be made in aqueous buffers, pH 4.0-8.0.
BIOLOGICAL ACTIVITY:
Inhibits human leukocyte elastase (K
i
=0.17nM) and human proteinase 3 (K
i
=0.42nM) .
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
HAZARD:
HARMFUL.
Product Description
Belongs to the chelonianin family of protease inhibitors and is involved in the physiological control of neutrophil serine proteinases, where it targets NE and proteinase 3. Shows antiprotease, antibacterial and antiinflammatory activity and primes innate immunity.
Product Specific Literature References
[1]
Elafin: an elastase-specific inhibitor of human skin. Purification, characterization, and complete amino acid sequence:
O. Wiedow, et al.; J. Biol. Chem.
265
, 14791 (1990)
Abstract
;
Full Text
[2]
Skin-derived antileucoproteases (SKALPs): characterization of two new elastase inhibitors from psoriatic epidermis:
J. Schalkwijk, et al.; Br. J. Dermatol.
122
, 631 (1990)
Abstract
[3]
Purification and characterization of elastase-specific inhibitor. Sequence homology with mucus proteinase inhibitor:
J.M. Sallenave and A.P. Ryle; Biol. Chem. Hoppe Seyler
372
, 13 (1991)
Abstract
[4]
Primary structure of the human elafin precursor preproelafin deduced from the nucleotide sequence of its gene and the presence of unique repetitive sequences in the prosegment:
Saheki, et al.; Biochem. Biophys. Res. Commun.
185
, 240 (1992)
Abstract
[5]
Kinetics of the inhibition of human leukocyte elastase by elafin, a 6-kilodalton elastase-specific inhibitor from human skin:
Q.L. Ying and S.R. Simon; Biochemistry
32
, 1866 (1993)
Abstract
[6]
SKALP/elafin: an elastase inhibitor from cultured human keratinocytes. Purification, cDNA sequence, and evidence for transglutaminase cross-linking:
H.O. Molhuizen, et al.; J. Biol. Chem.
268
, 12028 (1993)
Abstract
[7]
Characterization and gene sequence of the precursor of elafin, an elastase-specific inhibitor in bronchial secretions:
J.M. Sallenave and A. Silva; Am. J. Respir. Cell. Mol. Biol.
8
, 439 (1993)
Abstract
[8]
Kinetics of the inhibition of human leukocyte elastase by elafin, a 6-kilodalton elastase-specific inhibitor from human skin:
Q.L. Ying and S.R. Simon; Biochemistry
32
, 1866 (1993)
Abstract
[9]
Elastase inhibitor elafin is a new type of proteinase inhibitor which has a transglutaminase-mediated anchoring sequence termed "cementoin":
K. Nara, et al.; J. Biochem. (Tokyo)
115
, 441 (1994)
Abstract
[10]
Accelerated evolution in inhibitor domains of porcine elafin family members:
I. Tamechika, et al.; J. Biol. Chem.
271
, 7012 (1996)
Abstract
;
Full Text
[11]
Identification and sequence analysis of two new members of the SKALP/elafin and SPAI-2 gene family. Biochemical properties of the transglutaminase substrate motif and suggestions for a new nomenclature:
P.L. Zeeuwen, et al.; J. Biol. Chem.
272
, 20471 (1997)
Abstract
;
Full Text
[12]
The trappin gene family: proteins defined by an N-terminal transglutaminase substrate domain and a C-terminal four-disulphide core:
J. Schalkwijk, et al.; Biochem. J.
340
, 569 (1999)
Abstract
;
Full Text
[13]
The role of secretory leukocyte proteinase inhibitor and elafin (elastase-specific inhibitor/skin-derived antileukoprotease) as alarm antiproteinases in inflammatory lung disease:
J.M. Sallenave; Respir. Res.
1
, 87 (2000)
Abstract
[14]
Kinetics of the inhibition of proteinase 3 by elafin:
Q.L. Ying and S.R. Simon; Am. J. Respir. Cell Mol. Biol.
24
, 83 (2001)
Abstract
[15]
Trappin ovine molecule (TOM), the ovine ortholog of elafin, is an acute phase reactant in the lung:
T.I. Brown, et al.; Physiol. Genomics
19
, 11 (2004)
Abstract
;
Full Text
[16]
Elafin and Its Precursor Trappin-2 Still Inhibit Neutrophil Serine Proteinases when They Are Covalently Bound to Extracellular Matrix Proteins by Tissue Transglutaminase:
N. Guyot, et al.; Biochemistry
44
, 15610 (2005)
Abstract
[17]
SLPI and elafin: one glove, many fingers:
S.E. Williams, et al.; Clin. Sci. (Lond)
110
, 21 (2006)
Abstract
Further Categories Containing This Product:
Inflammation Other Products
•
Recombinant Proteins/Fusion Proteins
•
Proteases Other Products
ALX-380-072
Revised 05-Apr-08
Gallidermin
SYNONYMS
6L-Epidermin
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Antimicrobial Peptides & Proteins/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-380-072-M005
5 mg
120.00 USD
Product Specification
FORMULA:
C
98
H
141
N
25
O
23
S
4
MW:
2069.4
CAS NUMBER:
117978-77-5
SOURCE/HOST:
Isolated from
Staphylococcus gallinarum
Tü3928.
PURITY:
≥90% (HPLC)
APPEARANCE:
Lyophilized.
SOLUBILITY:
Soluble in water, 100% ethanol or methanol (1mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HAZARD:
TOXIC.
Product Description
Antibiotic peptide. Interferes with bacterial cell wall biosynthesis.
Product Specific Literature References
Gallidermin: a new lanthionine-containing polypeptide antibiotic:
R. Kellner, et al.; Eur. J. Biochem.
177
, 53 (1988)
Abstract
Isolation and characterization of genetically engineered gallidermin and epidermin analogs:
B. Ottenwalder, et al.; Appl. Environ. Microbiol.
61
, 3894 (1995)
Abstract
;
Full Text
Secretion of the lantibiotics epidermin and gallidermin: sequence analysis of the genes gdmT and gdmH, their influence on epidermin production and their regulation by EpiQ:
A. Peschel, et al.; Mol. Gen. Genet.
254
, 312 (1997)
Abstract
Identification of unusual amino acids in peptides using automated sequential Edman degradation coupled to direct detection by electrospray-ionization mass spectrometry:
T.B. Walk, et al.; Biopolymers
49
, 329 (1999)
Abstract
Dual role of GdmH in producer immunity and secretion of the Staphylococcal lantibiotics gallidermin and epidermin:
M. Hille, et al.; Appl. Environ. Microbiol.
67
, 1380 (2001)
Abstract
;
Full Text
Circumventing the effect of product toxicity: development of a novel two-stage production process for the lantibiotic gallidermin:
G. Valsesia, et al.; Appl. Environ. Microbiol.
73
, 1635 (2007)
Abstract
;
Full Text
ALX-210-376
Revised 19-Jun-07
Polyclonal Antibody to Secretory Leukocyte Proteinase Inhibitor (human)
SYNONYMS
anti-SLPI (human) PAb
anti-Antileukoproteinase 1 (human) PAb
anti-ALP (human) PAb
PRODUCT LINE
Immunology
PRODUCT CATEGORY
Antimicrobial Peptides & Proteins/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-376-C100
100 µg
220.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.1mg/ml.
PURITY DETAIL:
Protein G-affinity purified. 0.2µm-membrane filtered.
FORMULATION:
Liquid. In PBS containing 15mM sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa75-89 (P
75
VDTPNPTRRKPGKC
89
) of human SLPI (secretory leukocyte proteinase inhibitor).
SPECIFICITY:
Recognizes human SLPI.
APPLICATION:
ELISA
Immunohistochemistry (frozen sections (1:100-1:1'000), paraffin sections (1:100-1:1'000))
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Do not freeze.
Product Specific Literature References
Protease-protease inhibitor balance in the gastric mucosa. Influence of Helicobacter pylori infection:
M. Nilius, et al.; Adv. Exp. Med. Biol.
477
, 445 (2000)
Abstract
General Information
Secretory leukoprotease inhibitor (SLPI) is a low molecular weight serine proteinase inhibitor found on various mucosal surfaces and has been ascribed an important role in maintaining the protease-anti-protease balance of the airways. SLPI is produced by various epithelia and also possibly by neutrophils, mast cells and macrophages. Various studies have demonstrated the importance of serine proteinase inhibitors in regulating the activity of serine proteinases that are released by leukocytes during inflammation. Lipopolysaccharides (LPS) are able to up-regulate SPLI production in macrophages and addition of recombinant SLPI to human monocytes can down-regulate pro-inflammatory mediators such as tumor necrosis factor (TNF), presumably to limit self-damaging excessive inflammation. SLPI may also have a broad spectrum antibiotic activity that includes antiretroviral (e.g. HIV), bactericidal and antifungal activity.
SLPI is a cationic, non-glycosylated 11.7kDa protein (107aa)comprising two domains (trypsin inhibitory domain, aa26-83 and elastase inhibitory domain, aa84-132). Its major target is thought to be the human neutrophil elastase, but SLPI also inhibits the neutrophil proteinase cathepsin G, pancreatic trypsin and chymotrypsin, as well as mast cell chymase and tryptase.
General Literature References
Secretory leukoprotease inhibitor: a native antimicrobial protein presenting a new therapeutic option?
:
J.F. Tomee, et al.; Thorax
53
, 114 (1998), (Review)
Abstract
The role of secretory leukocyte proteinase inhibitor and elafin (elastase-specific inhibitor/skin-derived antileukoprotease) as alarm antiproteinases in inflammatory lung disease
:
J.M. Sallenave; Respir. Res.
1
, 87 (2000), (Review)
Abstract
;
Full Text
Antimicrobial activity of antiproteinases
:
J.M. Sallenave; Biochem. Soc. Trans.
30
, 111 (2002), (Review)
Abstract
;
Full Text
Novel roles of protease inhibitors in infection and inflammation
:
P.S. Hiemstra; Biochem. Soc. Trans.
30
, 116 (2002), (Review)
Abstract
;
Full Text
Further Categories Containing This Product:
Inflammation Other Products
•
Polyclonal Antibodies
•
Proteases Other Products
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