ALEXIS Biochemicals: Caspase-10/a (active) (human) (recombinant)

ALX-201-091

Revised 20-Oct-05

Caspase-10/a (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-091-U025		25 U	203.00 USD
ALX-201-091-U100		100 U	371.00 USD

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:	~8'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Product Specific Literature References

Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling: C. Vincenz & V.M. Dixit; J. Biol. Chem. 272, 6578 (1997) Abstract; Full Text
In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains: T. Fernandes-Alnemri, et al.; PNAS 93, 7464 (1996) Abstract

General Information

Caspase-10 (also known as Mch4 and Flice2) is a member of the interleukin-1b converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-10 exists in cells as an inactive proenzyme. During apoptosis, procaspase-10 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-10 consists of large (18kDa) and small (11kDa) subunits which associate to form the active enzyme.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q92851: Caspase-10 (human) (precursor)
AfCS Signalling Gateway link A000493: Caspase-10 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins/Fusion Proteins

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