ALEXIS Biochemicals: Caspase-1 (active) (human) (recombinant)

ALX-201-056

Revised 21-Jul-08

Caspase-1 (active) (human) (recombinant)
SYNONYMS	Interleukin 1β Converting Enzyme (active) (human) (recombinant) IL-1β Converting Enzyme (active) (human) (recombinant) ICE (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-056-U025		25 U	231.00 USD
ALX-201-056-U100		100 U	385.00 USD

Product Specification

EC:	3.4.22.36
SOURCE/HOST:	Produced in E. coli.
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:	~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:	Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL:	Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-pNA (Prod. No. ALX-260-026).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

General Information

Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P29466: Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492: Caspase-1 (mouse)

Further Categories Containing This Product:

Recombinant Proteins/Fusion Proteins • Inflammasome • Enzymes

another CS2 website

Online Product Ordering

Clipboard [0]

Your items will be kept only for the duration of your visit.