ALEXIS Biochemicals: Caspase-2 (active) (human) (recombinant)

ALX-201-057

Revised 30-Jun-08

Caspase-2 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-057-U025		25 U	203.00 USD
ALX-201-057-U100		100 U	371.00 USD

Product Specification

EC:	3.4.22.36
SOURCE/HOST:	Produced in E. coli.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:	10'000-13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VDVAD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:	Useful in screening capase inhibitors, studying enzyme regulation and kinetics, determining specificity of capase substrates, or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase-2 assay protocol, please refer to Caspase-2 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-214 or ALX-850-213).
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

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General Information

Caspase-2 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kDa) and small (12kDa) subunits which associate to form the active enzyme. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA (Prod. No. ALX-260-059). The signal can be detected by spectrophotometry.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P42575: Caspase-2 (human) (precursor)
AfCS Siganlling Gateway link A000497: Caspase-2 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins/Fusion Proteins

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