ALEXIS Biochemicals: Caspase-3 Fluorometric Assay Kit

ALX-850-216

Revised 24-Jun-08

Caspase-3 Fluorometric Assay Kit
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Kits & Sets

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-216-KI01		25 tests	175.00 USD

Product Specification

KIT/SET CONTAINS:	25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl DEVD-AFC (1mM), 100µl DTT (1M).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Store Lysis Buffer and 2X Reaction Buffer at 4°C after opening.

Product Description

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate DEVD-AFC.

Product Specific Literature References

Apoptosis and Id2 expression in diaphragm and soleus muscle from the emphysematous hamster: H. Degens, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R135 (2007) Abstract

General Information

DEVD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-3 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity.

MANUFACTURER

Manufactured by BioVision.

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