ALEXIS Biochemicals: Caspase-3 (active) (human) (recombinant) (high purity)

ALX-522-069

Revised 30-Jun-05

Caspase-3 (active) (human) (recombinant) (high purity)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-069-C005		5 µg	180.00 USD

Product Specification

MW:	~29kDa; α-subunit: ~17kDa; β-subunit: ~12kDa.
EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	0.1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM PIPES, pH 7.4, containing 10mM DTT, 100mM sodium chloride, 10% (w/v) sucrose, 0.1% (w/v) CHAPS and 1mM EDTA.
SPECIFIC ACTIVITY:	≥50'000U/mg. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate Ac-DEVD-AMC (Prod. No. ALX-260-031) per min. at 37°C in a buffered solution.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

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General Information

BACKGROUND/TECHNICAL INFORMATION	The rate of recombinant human caspase-3 enzymatic activity can be measured by the release of AMC from the Ac-DEVD-AMC (Prod. No. ALX-260-031) caspase substrate as emission at 440nm upon excitation at 380nm using a spectrofluorometer [1]. Swiss-Prot link: Caspase-3 (human) (precursor) AfCS Signalling Gateway link: Caspase-3 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Biochemical characteristics of caspases-3, -6, -7, and -8: H.R. Stennicke & G.S. Salvesen; J. Biol. Chem. 272, 25719 (1997) Abstract; Full Text

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Enzymes

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