ALEXIS Biochemicals: Caspase-3 (rat) (recombinant)

ALX-201-078

Revised 24-Jan-05

Caspase-3 (rat) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-078-C005		5 µg	320.00 USD

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	0.165mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. 5µg of caspase-3 in 50mM HEPES, pH 7.4, 100mM NaCI, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 50% glycerol.
SPECIFIC ACTIVITY:	≥500U/µg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase-substrate Z-DEVD-AMC per hour at 20°C in a reaction solution containing 20µM substrate, 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 10% glycerol.
APPLICATION:	In combination with caspase-3 activity assays, this product is useful in biological screening of caspase substrates and inhibitors. It can also be used as a positive control in caspase-3 assays as well as a positive control in Western blot.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stability at -20°C: 1-2 weeks
HANDLING:	Avoid freeze/thaw cycles.

Product Description

Contains both the proenzyme and the processed (active) form.

Product Specific Literature References

Presence of DNA fragmentation and lack of neuroprotective effect in DFF45 knockout mice subjected to traumatic brain injury: A.G. Yakovlev, et al.; Mol. Med. 7, 205 (2001) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Enzyme Evaluation Protocol (general)
This protocol can be used to measure caspase enzyme activity. A synthetic fluorogenic peptide, e. g. Ac-DEVD-AMC (Prod. No. ALX-260-031) is used as the caspase enzyme substrate. The enzyme cleaves the substrate releasing the fluorescent AMC. AMC release can be measured by spectrofluorometry using UV.
1. Add 20µM of Ac-DEVD-AMC to 1 ml of assay buffer (20mM PIPES, pH 7.2, 100mM sodium chloride, 10mM DTT, 1mM EDTA, 0.1% (w/v) CHAPS, 10% sucrose). Add the appropriate amount of active caspase to the mixture (e.g. 50ng/ml).
2. Incubate for 1 hour at 37°C.
3. Measure the AMC liberated from the Ac-DEVD-AMC using a spectrofluorometer with an excitation wavelength of 380nm and an emission wavelength of 440nm.

Swiss-Prot link P55213: Caspase-3 (rat)
AfCS Signalling Gateway link A000498: Caspase-3 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins/Fusion Proteins

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