ALEXIS Biochemicals: Caspase-4 (active) (human) (recombinant)

ALX-201-093

Revised 15-Apr-08

Caspase-4 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-093-U025		25 U	203.00 USD
ALX-201-093-U100		100 U	371.00 USD

Product Specification

EC:	3.4.22.57
SOURCE/HOST:	Produced in E. coli.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute with PBS containing 15% glycerol to 1U/µl.
SPECIFIC ACTIVITY:	~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:	Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

General Information

Caspase-4 is a member of the caspase family of cysteine proteases. It exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits characteristic of the native enzyme. The active caspase-4 is routinely tested for its ability to enzymatically cleave the substrate WEHD-AFC or WEHD-pNA.

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P49662: Caspase-4 (human) (precursor)
AfCS Signalling Gateway link A000499: Caspase-4 (mouse)

Further Categories Containing This Product:

Enzymes • Endoplasmatic Reticulum Stress • Recombinant Proteins/Fusion Proteins

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